Skip to main content

In Vitro Antioxidant Assays

  • Chapter
  • First Online:
Pharmacological Assays of Plant-Based Natural Products

Part of the book series: Progress in Drug Research ((PDR,volume 71))

Abstract

Various chemical in vitro assays have been developed to measure antioxidant capacities of plant products. Despite the recent popularity in the antioxidant research, the lack of standardized assays to compare research results from different research groups has been a major challenge. The examination of various antioxidant assays is required for the development of standard methods that are broadly applicable by researchers and industry. However, due to the complex nature of biological systems, there is no single universal method for measuring antioxidant capacity. Antioxidant methods such as DPPH ·, ABTS·+, nitric oxide, super oxide, metal chelating confirming the free radical scavenging property of the plants with widely used methods are simplified in this chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assay and an assay applicable to polyacrylamide gels. Analytical Biochemistry, 44(1), 276–287.

    Article  CAS  PubMed  Google Scholar 

  • Blois, M. S. (1958a). Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199–1200.

    Article  Google Scholar 

  • Blois, M. S. (1958b). Antioxidants determination by the use of a stable free radical. Nature, 181(4617), 1199–1200.

    Article  CAS  Google Scholar 

  • Buettner, G. R. (1993). The pecking order of free radicals and antioxidants: lipid peroxidation, α-tocopherol, and ascorbate. Archives of Biochemistry and Biophysics, 300, 535–543.

    Article  CAS  PubMed  Google Scholar 

  • Dinis, T. C. P., Madeira, V. M. C., & Almeida, L. M. (1994). Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161–169.

    Article  CAS  PubMed  Google Scholar 

  • Ruch, R. J., Cheng, S. J., & Klaunig, J. E. (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, 1003–1008.

    Google Scholar 

  • Ruberto, G., Baratta, M. T. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167–174.

    Google Scholar 

  • Huang, D., Ou, B., & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry, 53(6), 1841–1856.

    Article  CAS  Google Scholar 

  • Klein, S. M., Cohen, G., & Cederbaum, A. I. (1991). Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry, 20, 6006–6012.

    Article  Google Scholar 

  • Oyaizu, M. (1986). Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44(6), 307–315.

    Article  CAS  Google Scholar 

  • Phanikumar, G., Navya, K., Ramya, E. M., Venkataramana, M., Anand, T., Anand, T., & Anilakumar, K. R. (2013). DNA damage protecting and free radical scavenging properties of Terminalia arjuna bark in PC-12 cells and plasmid DNA. Free Radicals and Antioxidants, 3, 35–39.

    Article  Google Scholar 

  • Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337–341.

    Article  CAS  PubMed  Google Scholar 

  • Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agriculture and Food Chemistry, 53, 4290–4303.

    Article  CAS  Google Scholar 

  • Pulido, R., Bravo, L., & Sauro-Calixto, F. (2000). Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48(8), 3396–3402.

    Article  CAS  PubMed  Google Scholar 

  • Rajan, I., Rabindran, R., Jayasree, P. R., & Kumar, P. R. M. (2014). Antioxidant potential and oxidative DNA damage preventive activity of unexplored endemic species of Curcuma. Indian Journal of Experimental Biology, 52, 133–138.

    PubMed  Google Scholar 

  • Re, R., Pelligrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. A. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9), 1231–1237.

    Google Scholar 

  • Sreejayan, N., & Rao, M. N. A. (1997). Nitric oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology, 49(1), 105–107.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Parimelazhagan Thangaraj .

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Thangaraj, P. (2016). In Vitro Antioxidant Assays. In: Pharmacological Assays of Plant-Based Natural Products. Progress in Drug Research, vol 71. Springer, Cham. https://doi.org/10.1007/978-3-319-26811-8_9

Download citation

Publish with us

Policies and ethics