Abstract
Various chemical in vitro assays have been developed to measure antioxidant capacities of plant products. Despite the recent popularity in the antioxidant research, the lack of standardized assays to compare research results from different research groups has been a major challenge. The examination of various antioxidant assays is required for the development of standard methods that are broadly applicable by researchers and industry. However, due to the complex nature of biological systems, there is no single universal method for measuring antioxidant capacity. Antioxidant methods such as DPPH ·, ABTS·+, nitric oxide, super oxide, metal chelating confirming the free radical scavenging property of the plants with widely used methods are simplified in this chapter.
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References
Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: Improved assay and an assay applicable to polyacrylamide gels. Analytical Biochemistry, 44(1), 276–287.
Blois, M. S. (1958a). Antioxidants determination by the use of a stable free radical. Nature, 4617, 1199–1200.
Blois, M. S. (1958b). Antioxidants determination by the use of a stable free radical. Nature, 181(4617), 1199–1200.
Buettner, G. R. (1993). The pecking order of free radicals and antioxidants: lipid peroxidation, α-tocopherol, and ascorbate. Archives of Biochemistry and Biophysics, 300, 535–543.
Dinis, T. C. P., Madeira, V. M. C., & Almeida, L. M. (1994). Action of phenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161–169.
Ruch, R. J., Cheng, S. J., & Klaunig, J. E. (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis, 10, 1003–1008.
Ruberto, G., Baratta, M. T. (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167–174.
Huang, D., Ou, B., & Prior, R. L. (2005). The chemistry behind antioxidant capacity assays. Journal of Agriculture and Food Chemistry, 53(6), 1841–1856.
Klein, S. M., Cohen, G., & Cederbaum, A. I. (1991). Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry, 20, 6006–6012.
Oyaizu, M. (1986). Studies on products of browning reaction: Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44(6), 307–315.
Phanikumar, G., Navya, K., Ramya, E. M., Venkataramana, M., Anand, T., Anand, T., & Anilakumar, K. R. (2013). DNA damage protecting and free radical scavenging properties of Terminalia arjuna bark in PC-12 cells and plasmid DNA. Free Radicals and Antioxidants, 3, 35–39.
Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectophotometric quantitative of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337–341.
Prior, R. L., Wu, X., & Schaich, K. (2005). Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agriculture and Food Chemistry, 53, 4290–4303.
Pulido, R., Bravo, L., & Sauro-Calixto, F. (2000). Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48(8), 3396–3402.
Rajan, I., Rabindran, R., Jayasree, P. R., & Kumar, P. R. M. (2014). Antioxidant potential and oxidative DNA damage preventive activity of unexplored endemic species of Curcuma. Indian Journal of Experimental Biology, 52, 133–138.
Re, R., Pelligrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. A. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9), 1231–1237.
Sreejayan, N., & Rao, M. N. A. (1997). Nitric oxide scavenging by curcuminoids. Journal of Pharmacy and Pharmacology, 49(1), 105–107.
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Thangaraj, P. (2016). In Vitro Antioxidant Assays. In: Pharmacological Assays of Plant-Based Natural Products. Progress in Drug Research, vol 71. Springer, Cham. https://doi.org/10.1007/978-3-319-26811-8_9
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DOI: https://doi.org/10.1007/978-3-319-26811-8_9
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