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Sperm Preparation for Intrauterine Insemination Using Density Gradient Separation

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Andrological Evaluation of Male Infertility

Abstract

Sperm washing is performed to remove seminal plasma and increase sperm density prior to intrauterine insemination (IUI). Gradient sperm washing is highly effective at isolating motile sperm from the ejaculate of most men. IUI may be indicated in cases of poor postcoital testing due to hostile or absent cervical mucus, oligospermia, or the presence of sperm antibodies. It may be used in conjunction with artificial insemination or IVF procedures.

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References

  1. Beydola T, Sharma RK, Lee W, Agarwal A. Sperm preparation and selection techniques. In: Rizk B, Aziz N, Agarwal A, Sabanegh E, editors. Male infertility practice. New Delhi: Jaypee Brothers Medical Publishers; 2012.

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  2. Agarwal A, Allamaneni S. Artificial insemination. Section 6: infertility and recurrent pregnancy loss. Clinical reproductive medicine and surgery: Tommaso Falcone and William Hurd. Elsevier publishers.

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Author information

Authors and Affiliations

  1. Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA

    Ashok Agarwal PhD, HCLD (ABB), ELD (ACE), Sajal Gupta MD, MSc, TS (ABB) & Rakesh Sharma PhD

Authors
  1. Ashok Agarwal PhD, HCLD (ABB), ELD (ACE)
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  2. Sajal Gupta MD, MSc, TS (ABB)
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  3. Rakesh Sharma PhD
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Corresponding author

Correspondence to Ashok Agarwal PhD, HCLD (ABB), ELD (ACE) .

Editor information

Editors and Affiliations

  1. Cleveland Clinic, American Center for Reproductive Medicine, Cleveland, Ohio, USA

    Ashok Agarwal

  2. Cleveland Clinic, American Center for Reproductive Medicine, CLEVELAND, Ohio, USA

    Sajal Gupta

  3. Cleveland Clinic, American Center for Reproductive Medicine, Cleveland, Ohio, USA

    Rakesh Sharma

Cleveland Clinic

Cleveland Clinic

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© 2016 Springer International Publishing Switzerland

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Agarwal, A., Gupta, S., Sharma, R. (2016). Sperm Preparation for Intrauterine Insemination Using Density Gradient Separation. In: Agarwal, A., Gupta, S., Sharma, R. (eds) Andrological Evaluation of Male Infertility. Springer, Cham. https://doi.org/10.1007/978-3-319-26797-5_14

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  • DOI: https://doi.org/10.1007/978-3-319-26797-5_14

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-26795-1

  • Online ISBN: 978-3-319-26797-5

  • eBook Packages: MedicineMedicine (R0)

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