Abstract
Apoptosis is an imperative constituent of various processes including proper progression and functioning of the immune system, embryonic development as well as chemical-induced cell death. Improper apoptosis is a reason in numerous human/animal’s conditions involving ischemic damage, neurodegenerative diseases, autoimmune disorders and various types of cancer. An outstanding feature of neurodegenerative diseases is the loss of specific neuronal populations. Thus, the detection of the dead cells is a necessity. This paper proposes a novel algorithm to achieve the dead cells detection based on color intensity and contrast changes and aims for fully automatic apoptosis detection based on image analysis method. A stained cultures images using Caspase stain of albino rats hippocampus specimens using light microscope (total 21 images) were used to evaluate the system performance. The results proved that the proposed system is efficient as it achieved high accuracy (98.89 ± 0.76 %) and specificity (99.36 ± 0.63 %) and good mean sensitivity level of (72.34 ± 19.85 %).
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Acknowledgment
This paper has been elaborated in the framework of the project “New creative teams in priorities of scientific research”, reg. no. CZ.1.07/2.3.00/30.0055, supported by Operational Programme Education for Competitiveness and co-financed by the European Social Fund and the state budget of the Czech Republic and supported by the IT4Innovations Centre of Excellence project (CZ.1.05/1.1.00/02.0070), funded by the European Regional Development Fund and the national budget of the Czech Republic via the Research and Development for Innovations Operational Programme and by Project SP2015/146 “Parallel processing of Big data 2” of the Student Grant System, VSB—Technical University of Ostrava.
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Kotyk, T. et al. (2016). Detection of Dead Stained Microscopic Cells Based on Color Intensity and Contrast. In: Gaber, T., Hassanien, A., El-Bendary, N., Dey, N. (eds) The 1st International Conference on Advanced Intelligent System and Informatics (AISI2015), November 28-30, 2015, Beni Suef, Egypt. Advances in Intelligent Systems and Computing, vol 407. Springer, Cham. https://doi.org/10.1007/978-3-319-26690-9_6
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