Abstract
A storable whole-cell biosensor has been a challenge in the whole-cell biosensor research. We developed a long-term storable whole-cell biosensor using the true slime mould, Physarum polycephalum, for toxicity detection. The cell is interfaced to a microfluidic device with impedance measurement system. The oscillatory activity of the cell when exposed to various concentrations of 2,4-dinitrophenol (DNP) is investigated. It has been demonstrated that the Physarum cell can be dry-stored in the device for months and used as bionsensor after revived with rehydration. This is the first implementation of storable whole-cell biosensor for toxicity detection use, and it suggests that the development of long-term storable, and potentially portable, whole-cell biosensor for general toxicity prescreening is possible.
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Acknowledgments
The authors wish to thank Jeff Jones and Nurul Yunus for their comments on an earlier version of this paper. This research is supported by the Life Sciences Interfaces Forum, University of Southampton, UK and the Leverhulme Trust funded project “Mould intelligence: biological amorphous robots”.
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Tsuda, S., Zauner, KP., Morgan, H. (2016). Long-Term Storable Microfluidic Whole-Cell Biosensor Using Physarum polycephalum for Toxicity Prescreening. In: Adamatzky, A. (eds) Advances in Physarum Machines. Emergence, Complexity and Computation, vol 21. Springer, Cham. https://doi.org/10.1007/978-3-319-26662-6_8
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DOI: https://doi.org/10.1007/978-3-319-26662-6_8
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