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Whether in the field of medicine, mathematics, botany or architecture: a systematic investigation into the interconnections between knowledge and gardens has yet to be undertaken. Despite numerous case studies, basic analyses are not even available for individual knowledge constellations, for generating and systematising, transferring and applying specific forms of knowledge to horticulture and garden art. The interdisciplinary symposium “Gardening and Knowledge. Landscape Design and the Sciences in the Early Modern Period”, funded by the Volkswagen Foundation, was intended to fill a sensitive gap spanning the sixteenth to eighteenth centuries. It was jointly organised by the Centre of Garden Art and Landscape Architecture (CGL) of the Leibniz Universität Hannover and the Interdisciplinary Centre for Science and Technology Studies (IZTW) of the Bergische Universität Wuppertal. This volume is the result of the symposium held at the Leibniz Universität in September 2012.