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Applications of Mass Spectrometry in Microbiology pp 305–316Cite as

Detection of β-Lactamases and Their Activity Using MALDI-TOF MS

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Abstract

β-Lactamases, especially carbapenemases, represent a current threat to human medicine. They are also spread in animals, farms, and wildlife. Therefore, their fast and reliable detection is important for prevention of their uncontrolled spread. Here, we describe two methods based on matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry allowing functional detection of hydrolysis products of β-lactams as well as an assay for direct visualization of the molecular mass of lactamase. Using antibiotic molecular signal monitoring, fast identification of β-lactamases important for treatment and setting up barrier precautions preventing their spread is possible. On the contrary, direct enzyme molecular signal monitoring is reliable for epidemiological studies, typing of the isolates, and precise identification of β-lactamase type.

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Author information

Authors and Affiliations

  1. Biomedical Center and Department of Microbiology, Charles University in Prague, Alej Svobody 76, 323 00, Plzen, Czech Republic

    Jaroslav Hrabak & Costas C. Papagiannitsis

  2. RCPTM, Department of Analytical Chemistry, Palacky University, 17.listopadu 12, 771 46, Olomouc, Czech Republic

    Vladimír Havlicek

Authors
  1. Jaroslav Hrabak
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  2. Vladimír Havlicek
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  3. Costas C. Papagiannitsis
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Corresponding author

Correspondence to Jaroslav Hrabak .

Editor information

Editors and Affiliations

  1. Johns Hopkins University, Applied Physics Laboratory, Laurel, Maryland, USA

    Plamen Demirev

  2. Arizona State University, School of Math and Natural Sciences, Phoenix, Arizona, USA

    Todd R. Sandrin

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Hrabak, J., Havlicek, V., Papagiannitsis, C. (2016). Detection of β-Lactamases and Their Activity Using MALDI-TOF MS. In: Demirev, P., Sandrin, T. (eds) Applications of Mass Spectrometry in Microbiology. Springer, Cham. https://doi.org/10.1007/978-3-319-26070-9_12

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