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Stem Cells and Cardiac Regeneration pp 31–43Cite as

Induced Pluripotent Stem Cells for Cardiac Regeneration

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Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Induced pluripotent stem (iPS) cells are a type of pluripotent stem cell artificially derived from a non-pluripotent cell—typically an adult somatic cell—by inducing a “forced” expression of specific genes. Typically, iPS cells are generated by retroviral induction of transcription factors, OCT (octamer-binding transcription factor)-4, SRY (sex determining region Y)-box 2 also known as SOX2, kruppel-like factor (KLF)-4, and c-MYC, in fibroblasts. The development of iPS cells could be a strategy to overcome the limitations of human embryonic stem cells. iPS cells can replace animal and ES experiments in drug development and toxicity tests and for testing mechanicistic hypotheses of diseases. Although the creation of multiple lineages with iPS cells can seem limitless, a number of challenges need to be addressed in order to effectively use these cell lines for disease modeling. These include the low efficiency of iPS cell generation without genetic alterations, the possibility of tumor formation in vivo, the random integration of retroviral-based delivery vectors into the genome, and unregulated growth of the remaining cells that are partially reprogrammed and refractory to differentiation. The establishment of protein or RNA-based reprogramming strategies will help generate human iPS cells without permanent genetic alterations for future development of personalized medicine.

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Authors and Affiliations

  1. Institute of Cardiology, Department of Neurosciences and Imaging Laboratory of Experimental Cardiology, Center of Excellence on Aging—CESI, “G. d’Annunzio” University, Chieti Scalo, Italy

    Rosalinda Madonna

  2. Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, Hungary

    Peter Ferdinandy

  3. Pharmahungary Group, Szeged, Hungary

    Peter Ferdinandy

  4. Institute of Physiology, Justus-Liebig Giessen University of Giessen, Giessen, Germany

    Rainer Schulz

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  1. Rosalinda Madonna
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  2. Peter Ferdinandy
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  3. Rainer Schulz
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Correspondence to Rosalinda Madonna .

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Editors and Affiliations

  1. Institute of Cardiology, Department of Neurosciences and Imaging Laboratory of Experimental Cardiology, "G. d'Annunzio" University, Chieti Scalo, Chieti, Italy

    Rosalinda Madonna

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Madonna, R., Ferdinandy, P., Schulz, R. (2016). Induced Pluripotent Stem Cells for Cardiac Regeneration. In: Madonna, R. (eds) Stem Cells and Cardiac Regeneration. Stem Cell Biology and Regenerative Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-25427-2_3

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