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Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis

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Book cover The Physiology of Microalgae

Part of the book series: Developments in Applied Phycology ((DAPH,volume 6))

Abstract

Extracellular polysaccharides (EPSs) produced by microalgae and cyanobacteria are molecules with a great ecological significance for the producing organisms, serving in a wide array of biological processes and increasing the organism tolerance to environmental stresses. In addition, due to their distinctive chemical, rheological properties and the biological activity of some of them, these macromolecules could find application in industrial, pharmaceutical and medical fields. In this chapter, the current knowledge regarding the structure and composition of EPSs, the factors eliciting their synthesis, and the related codifying genes involved is reviewed. It is also underlined and discussed how, notwithstanding the huge number of studies available, different aspects still need to be clarified. For example, there are limited informations concerning EPS synthetic pathways, and how these processes are influenced by environmental factors. Expand the knowledge on these aspects could help elaborating biotechnological strategies to increase the yields and direct the synthesis to polymers with desired characteristics.

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Notes

  1. 1.

    Wherever possible the currently accepted names for species are used. The name used in the paper cited is also indicated. For details of names see chapter “Systematics, Taxonomy and Species Names: Do They Matter?” of this book (Borowitzka 2016).

References

  • Abdullahi AS, Underwood GCJ, Gretz MR (2006) Extracellular matrix assembly in diatoms (Bacillariophyceae). V. Environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum. J Phycol 42:363–378

    Article  CAS  Google Scholar 

  • Aboal M, Marco S, Chaves E, Mulero I, Garcia-Ayala A (2012) Ultrastructure and function of stalks of the diatom Didymosphenia geminata. Hydrobiologia 695:17–24

    Article  CAS  Google Scholar 

  • Abreu AP, Fernandes B, Vincente AA, Teixeira J, Dragone G (2012) Mixotrophic cultivation of Chlorella vulgaris using industrial dairy wast as organic carbon source. Bioresour Technol 118:61–66

    Article  CAS  PubMed  Google Scholar 

  • Adhikary SP, Weckesser J, Jürgens UJ, Golecki JR, Borowiak D (1986) Isolation and chemical characterization of the sheath from the cyanobacterium Chroococcus minutus SAG B.41.79. J Gen Microbiol 132:2595–2599

    CAS  Google Scholar 

  • Alcoverro T, Conte E, Mazzella L (2000) Production of mucilage by the Adriatic epipelic diatom Cylindrotheca closterium (Bacillariophyceae) under nutrient limitation. J Phycol 36:1087–1095

    Article  CAS  Google Scholar 

  • Allan GG, Lewin J, Johnson PG (1972) Marine polymers. IV. Diatom polysaccharides. Bot Mar 15:102–108

    Article  CAS  Google Scholar 

  • Allard B, Guillot JP, Casadeval E (1987) The production of extracellular exopolysaccharide by fresh water microalgae: investigation of the polysaccharide components. In: Grassi G, Delmon B, Molle JF, Zibetta H (eds) Biomass for energy and industry. Elsevier Applied Science, London, pp 599–603

    Google Scholar 

  • Angelis S, Novak AC, Sydney EB, Soccol VT, Carvalho JC, Pandey A, Noseda MD, Tholoz JL, Lorquin J, Soccol R (2012) Co-culture of microalgae, cyanobacteria, and macromycetes for exopolysaccharides production: process preliminary optimization and partial characterization. Appl Biochem Biotechnol 167:1092–1106

    Article  CAS  PubMed  Google Scholar 

  • Arad (Malis) S, Levy-Ontman O (2010) Red microalgal cell wall polysaccharides: biotechnological aspects. Curr Opin Biotechnol 21:358–364

    Article  CAS  Google Scholar 

  • Arad (Malis) S (1988) Production of sulfated polysaccharides from red unicellular algae. In: Stadler T, Mollion J, Verduset MC (eds) Algal biotechnology—an interdisciplinary perspective. Elsevier Applied Science, pp 65–87

    Google Scholar 

  • Arad (Malis) S, Levy-Ontman O (2013) Sulfated polysaccharides in the cell-wall of red microalgae. In: Thomas S, Durand D, Chassenieux D, Jyotishkumar P (eds) Handbook of biopolymer-based materials: from blends and composites to gels and complex networks, 1st edn. Wiley VGH Verlag, Weinheim, pp 351–370

    Chapter  Google Scholar 

  • Arad (Malis) S, Adda M, Cohen E (1985) The potential of production of sulphated polysaccharides from Porphyridium. Plant Soil 89:117–127

    Article  Google Scholar 

  • Arad (Malis) S, Friedman (Dahan) O, Rotem A (1988) Effect of nitrogen on polysaccharide production in a Porphyridium sp. Appl Environ Microbiol 54:2411–2414

    Google Scholar 

  • Arad (Malis) S, Lerental (Brown) Y, Dubinsky O (1992) Effect of nitrate and sulphate starvation on polysaccharide formation in Rhodella reticulata. Bioresour Technol 42:141–148

    Article  Google Scholar 

  • Arad (Malis) S, Simon-Berkovitch B, Dubinsky O (1993) A modified cell wall mutant of the red microalga Rhodella reticulata resistant to the herbicide 2,6-dichlorobenzonitrile. J Phycol 29:309–313

    Article  Google Scholar 

  • Arad (Malis) S, Kolani R, Simon-Berkovitch B, Sivan A (1994) Inhibition by DCB of cell wall polysaccharide formation in the red microalga Porphyridium sp. (Rhodophyta). Phycologia 33:158–162

    Article  Google Scholar 

  • Ascencio F, Gama NL, De Philippis R, Ho B (2004) Effectiveness of Cyanothece spp. and Cyanospira capsulata exocellular polysaccharides as antiadhesive agents for blocking attachment of Helicobacter pylori to human gastric cells. Folia Microbiol 49:64–70

    Article  CAS  Google Scholar 

  • Ball SG, Dirick L, Decq A, Martiat J-C, Matagne R (1990) Physiology of starch storage in the monocellular alga Chlamydomonas reinhardtii. Plant Sci 66(1):1–9

    Google Scholar 

  • Banerjee A, Sharma R, Chisti Y, Banerjee UC (2002) Botryococcus braunii: a renewable source of hydrocarbons and other chemicals. Crit Rev Biotechnol 22(3):245–279

    Google Scholar 

  • Banks JA, Nishiyama T, Hasebe M, Bowman JL, Gribskov M et al (2011) The Selaginella genome identifies genetic changes associated with the evolution of vascular plants. Science 332:960–963

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Barclay RW, Lewin RA (1985) Microalgal polysaccharide production for the conditioning of agricultural soils. Plant Soil 88:159–169

    Article  CAS  Google Scholar 

  • Bar-Or Y, Shilo M (1987) Characterization of macromolecular flocculants produced by Phormidium sp. strain J-1 and by Arabodopsis circularis PCC 6720. Appl Environ Microbiol 53:2226–2230

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bell WH (1983) Bacterial utilization of algal extracellular products. 3. The specificity of algal-bacterial interaction. Limnol Oceanogr 28:1131–1143

    Article  Google Scholar 

  • Bellezza S, Albertano P, De Philippis R, Paradossi G (2006) Exopolysaccharides of two cyanobacterial strains from Roman hypogea. Geomicrobiol J 23:301–310

    Article  CAS  Google Scholar 

  • Bellinger BJ, Abdullahi AS, Gretz MR, Underwood GJC (2005) Biofilm polymers: relationship between carbohydrate polymers from estuarine mudflats and unialgal cultures of benthic diatoms. Aquat Microb Ecol 38:169–180

    Article  Google Scholar 

  • Bender J, Rodriguez-Eaton, Ekanemesang UM, Philipps P (1994) Characterization of bioflocculants produced by the cyanobacterial component of different microbial mat. Appl Environ Microbiol 60:2311–2315

    CAS  PubMed  PubMed Central  Google Scholar 

  • Bermùdez J, Rosales N, Loreto C, Briceño B, Morales E (2004) Exopolysaccharide, pigment and protein production by the marine microalga Chroomonas sp. in semicontinuous cultures. World J Microbiol Biotechnol 20:179–183

    Article  Google Scholar 

  • Bertocchi C, Navarini L, Cesáro A (1990) Polysaccharides from cyanobacteria. Carbohydr Polym 12:127–153

    Article  CAS  Google Scholar 

  • Billi D, Potts M (2002) Life and death of dried prokaryotes. Res Microbiol 153:7–22

    Article  CAS  PubMed  Google Scholar 

  • Bixler HJ, Porse H (2010) A decade of change of the seaweed hydrocolloid industry. J Appl Phycol 23:321–335

    Article  Google Scholar 

  • Blanc G, Agarkova I, Grimwood J, Kuo A, Brueggeman A, Dunigan DD, Gurnon J, Ladunga I, Lindquist E, Lucas S, Pangilinan J, Pröschold T, Salamov A, Schmutz J, Weeks D, Yamada T, Lomsadze A, Borodovsky M, Claverie JM, Grigoriev IV, Van Etten JL (2012) The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation. Genome Biol 13(5):R39

    Google Scholar 

  • Blanc G, Duncan GA, Agarkova I, Borodovsky M, Gurnon J (2010) The Chlorella variabilis NC64A genome reveals adaptation to photosymbiosis, coevolution with viruses, and cryptic sex. Virology papers. Paper 234

    Google Scholar 

  • Borowitzka MA (2016) Systematics, taxonomy and species names: do they matter? In: Borowitzka MA, Beardall J, Raven JA (eds) The physiology of microalgae. Springer, Dordrecht, pp 655–681

    Google Scholar 

  • Brányiková I, Maršálková B, Doucha J, Brányik T, Bišová K, Zachleder V, Vítová M (2011) Microalgae—novel highly efficient starch producers. Biotechnol Bioeng 108:766–776

    Google Scholar 

  • Brosch-Solomon S, Höfterberger M, Holzinger A, Lütz-Meindl U (1998) Ultrastructural localization of polysaccharides and N-acetyl-D-galactosamine in the secretory pathway of green algae (Desmidiaceae). J Exp Bot 49:145–153

    Google Scholar 

  • Brown MR (1991) The amino acid and sugar composition of 16 species of microalgae used in mariculture. J Exp Mar Biol Ecol 145:79–99

    Google Scholar 

  • Bruckner CG, Bahulikar R, Rahalkar M, Schink B, Kroth P (2008) Bacteria associated with benthic diatoms from lake Constance: phylogeny and influences on diatom growth and secretion of extracellular polymeric substances. Appl Environ Microbiol 74:7740–7749

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cade-Menun BJ, Paytan A (2010) Nutrient temperature and light stress alter phosphorus and carbon forms in culture-grown algae. Mar Chem 121:27–36

    Article  CAS  Google Scholar 

  • Cadoret J-P, Garnier M, Saint-Jean B (2012) Microalgae, functional genomics and biotechnology. Adv Ecol Res 64:285–341

    Google Scholar 

  • Campbell JA, Davies GJ, Bulone V, Henrissat B (1997) A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem J 326:929–942

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, Henrissat B (2009) The carbohydrate-active enzymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res 37:D233–D238

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Carvalho A, Monteiro C, Malcata F (2009) Simultaneous effect of irradiance and temperature on biochemical composition of the microalga Pavlova lutheri. J Appl Phycol 21:543–552

    Article  Google Scholar 

  • Chi Z, Su CD, Lu WD (2007) A new exopolysaccharide produced by marine Cyanothece sp. 113. Bioresour Technol 98:1329–1332

    Article  CAS  PubMed  Google Scholar 

  • Chiovitti A, Higgins MJ, Harper RE, Wetherbee R, Bacic A (2003) The complex polysaccharides of the raphid diatom Pinnularia viridis (Bacillariophyceae). J Phycol 39(3):543–554

    Google Scholar 

  • Choi SP, Nguyen MT, Sim SJ (2010) Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. Bioresour Technol 101:5330–5336

    Google Scholar 

  • Choix FJ, de Bashan LE, Bashan Y (2012) Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions. Enzyme Microb Technol 51:300–309

    Article  CAS  PubMed  Google Scholar 

  • Coesel PFM, Wardenaar K (1994) Light-limited growth and photosynthetic characteristics of two planktonic desmid species. Freshw Biol 31:221–226

    Article  Google Scholar 

  • Cohen (Katz) A, Arad (Malis) S (1998) Biosynthesis of the cell-wall polysaccharide in the red microalga Rhodella reticulata. Isr J Plant Sci 46:144–153

    Google Scholar 

  • Colica G, De Philippis R (2013) Exopolysaccharides from cyanobacteria and their possible industrial applications. In: Sharma NK, Rai AK, Stal LJ (eds) Cyanobacteria an economic perspective. Wiley, Chichester, pp 197–208

    Chapter  Google Scholar 

  • Coutinho PM, Deleury E, Davies GJ, Henrissat B (2003) An evolving hierarchical family classification for glycosyltransferases. J Mol Biol 328:307–317

    Article  CAS  PubMed  Google Scholar 

  • Cuthbertson L, Mainprize IL, Naismith JH, Whitfield C (2009) Pivotal roles of the outer membrane polysaccharide export and polysaccharide copolymerase protein families in export of extracellular polysaccharides in gram-negative bacteria. Mol Biol Rev 73:155–177

    Article  CAS  Google Scholar 

  • Cuthbertson L, Kos V, Whitfield C (2010) ABC transporters involved in export of cell surface glycoconjugates. Microb Mol Biol Rev 74:341–362

    Article  CAS  Google Scholar 

  • Daniel GF, Chamberlain AHL, Jones EBG (1987) Cytochemical and electron microscopical observations on the adhesive materials of marine fouling diatoms. Br Phycol J 22:101–118

    Article  Google Scholar 

  • Davis KD (2012) Combining polysaccharide biosynthesis and transport in a single enzyme: dual-function cell wall glycan synthases. Front Plant Sci 3:138

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Decho AW (1990) Exopolymer microenvironments of microbial flora: multiple and interactive effects on trophic relationships. Limnol Oceanogr 38(8):1633–1645

    Google Scholar 

  • De Jesus Raposo MF, de Morais RMSC, de Morais AMMB (2013) Bioactivity and applications of sulphated polysaccharides from marine microalgae. Mar Drugs 11:233–252

    Article  PubMed Central  Google Scholar 

  • De Philippis R, Vincenzini M (1998) Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiol Rev 22:151–175

    Article  Google Scholar 

  • De Philippis R, Sili C, Tassinato G, Vincenzini M, Materassi R (1991) Effects of growth conditions on exopolysaccharide production by Cyanospira capsulata. Bioresour Technol 38:101–104

    Article  Google Scholar 

  • De Philippis R, Sili C, Vincenzini M (1992) Glycogen and poly-β-hydroxybutyrate synthesis in Spirulina maxima. J Gen Microbiol 138:1623–1628

    Article  Google Scholar 

  • De Philippis R, Margheri MC, Pelosi E, Ventura S (1993) Exopolysaccharide production by an unicellular cyanobacterium isolated from a hypersaline habitat. J Appl Phycol 5:387–394

    Article  Google Scholar 

  • De Philippis R, Sili C, Vincenzini M (1996) Response of an exopolysaccharide-producing heterocystous cyanobacteria. J Appl Phycol 8:275–281

    Article  Google Scholar 

  • De Philippis R, Margheri MC, Materassi R, Vincenzini M (1998) Potential of unicellular cyanobacteria from saline environments as exopolysaccharide producers. Appl Environ Microbiol 64:1130–1132

    PubMed  PubMed Central  Google Scholar 

  • De Philippis R, Ena A, Paperi R, Sili C, Vincenzini M (2000) Assessment of the potential of Nostoc strains from the Pasteur Culture Collection for the production of polysaccharides of applied interest. J Appl Phycol 12:401–407

    Article  Google Scholar 

  • De Philippis R, Sili C, Paperi R, Vincenzini M (2001) Exopolysaccharide-producing cyanobacteria and their possible exploitation: a review. J Appl Phycol 13:293–299

    Article  Google Scholar 

  • De Philippis R, Colica G, Micheletti E (2011) Exopolysaccharide-producing cyanobacteria in heavy metal removal from water: molecular basis and practical applicability of the biosorption process. Appl Microbiol Biotechnol 92:697–708

    Article  CAS  PubMed  Google Scholar 

  • Demura M, Ioki M, Kawachi M, Nakajima N, Watanabe M (2014) Desiccation tolerance of Botryococcus braunii (Trebouxiophyceae, Chlorophyta) and extreme temperature tolerance of dehydrated cells. J Appl Phycol 26:49–53

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Di Pippo F, Ellwood NTW, Gismondi A, Bruno L, Rossi F, Magni P, De Philippis R (2013) Characterization of exopolysaccharides produced by seven biofilm-forming cyanobacterial strains for biotechnological applications. J Appl Phycol 25:1697–1708

    Article  CAS  Google Scholar 

  • Domozych DS (1999) Disruption of Golgi apparatus and secretory mechanism of the desmid, Closterium acerosum, by brefeldin-A. J Exp Bot 50:1323–1330

    CAS  Google Scholar 

  • Domozych DS, Kort S, Benton S, Yu T (2005) The extracellular polymeric substance of the green alga Penium margaritaceum and its role in biofilm formation. Biofilms 2:129–144

    Article  Google Scholar 

  • El-Sheekh MM, Khairy HM, El-Shenody R (2012) Algal production of extra and intra-cellular polysaccharides as an adaptative response to the toxin crude extract of Microcystis aeruginosa. Iran J Chem Eng 9:10–17

    Google Scholar 

  • Fattom A, Shilo M (1984) Phormidium J-1 bioflocculant: production and activity. Arch Microbiol 139:421–426

    Google Scholar 

  • Fernandes HL, Tomé MM, Lupi FM, Fialho AM, Sá-Correia I, Novais JM (1989) Biosynthesis of high concentrations of an exopolysaccharide during the cultivation of the microalga Botryococcus braunii. Biotechnol Lett 11:433–436

    Article  CAS  Google Scholar 

  • Filali-Mouhim R, Cornet JF, Fontaine T, Fournet B, Dubertret G (1993) Production, isolation and preliminary characterization of the exopolysaccharides from the cyanobacterium Spirulina platensis. Biotechnol Lett 15:567–572

    Article  CAS  Google Scholar 

  • Fischer D, Schlösser UG, Pohl P (1997) Exopolysaccharide production by cyanobacteria grown in closed photobioreactors and immobilized using white cotton towelling. J Appl Phycol 9:205–213

    Article  CAS  Google Scholar 

  • Flaibani A, Olsen Y, Painter TJ (1989) Polysaccharides in desert reclamation: composition of exocellular proteoglycan complexes produced by filamentous blue-green and unicellular green edaphic algae. Carbohydr Res 190:235–248

    Article  CAS  Google Scholar 

  • Forni C, Telo FR, Caiola MG (1997) Comparative analysis of the polysaccharides produced by different species of Microcystis (Chroococcales, Cyanophyta). Phycologia 36:181–185

    Article  Google Scholar 

  • Fouchard S, Hemschemeier A, Caruana A, Pruvost J, Legrand J, Happe T, Peltier G, Cournac L (2005) Autotrophic and mixotrophic hydrogen photoproduction in sulfur-deprived Chlamydomonas cells. Appl Environ Microbiol 71:6199–6205

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Freire-Nordi CS, Vieira AAH, Nakaie CR, Nascimento OR (2006) Effect of polysaccharide capsule of the microalgae Staurastrum iversenii var. americanum on diffusion of charged and uncharged molecules, using EPS technique. Braz J Phys 36(1A):75–82

    Article  CAS  Google Scholar 

  • Friedman O, Dubinsky Z, Arad S (1991) Effect of light intensity on growth and polysaccharide production in red and blue-green rhodophyta unicells. Bioresour Technol 38:105–110

    Article  CAS  Google Scholar 

  • Gantar M, Rowell P, Kerby NW, Sutherland IW (1995) Role of extracellular polysaccharide in the colonization of wheat (Triticum vulgare L.) roots by N2-fixing cyanobacteria. Biol Fertil Soils 19:41–44

    Article  CAS  Google Scholar 

  • Garcia-Pichel F, Castenholz RW (1991) Characterization and biological implications of scytonemin, a cyanobacterial sheath pigment. J Phycol 27:395–409

    Article  CAS  Google Scholar 

  • Garcia-Pichel F, Wingard CE, Castenholz RW (1993) Evidence regarding the UV sunscreen role of a mycosporine-like compound in the cyanobacterium Gloeocapsa sp. Appl Environ Microbiol 59:170–176

    Google Scholar 

  • Garozzo D, Impallomeni G, Spina E, Sturiale L, Cesaro A, Cescutti P (1995) Identification of N-acetylglucosamine and 4-O-[1-carboxyethyl]mannose in the exopolysaccharide from Cyanospira capsulata. Carbohydr Res 270:97–106

    Article  CAS  Google Scholar 

  • Geresh S, Arad (Malis) S, Levy-Ontman O, Zhang W, Tekoah Y, Glaser R (2009) Isolation and characterization of poly- and oligosaccharides from the red microalga Porphyridium sp. Carbohydr Res 344:343–349

    Google Scholar 

  • Gloaguen V, Morvan H, Hoffmann L (1995) Released and capsular polysaccharides of Oscillatoriaceae (Cyanophyceae, Cyanobacteria). Algol Stud 78:53–69

    Google Scholar 

  • Gloaguen V, Ruiz G, Morvan H, Mouradi – Givernaud A, Maes E, Krausz P, Strecker G (2004) The extracellular polysaccharide of Porphyridium sp. an NMR study of lithium resistant oligosaccharidic fragments. Carbohydr Res 339:97–103

    Article  CAS  PubMed  Google Scholar 

  • Gouvea SP, Vieira AAH, Lombardi AT (2005) Copper and cadmium complexation by high molecular weight materials of dominant microalgae and of water from eutrophic reservoir. Chemosphere 60:1332–1339

    Article  CAS  PubMed  Google Scholar 

  • Guerrini F, Cangini M, Boni L, Trost P, Pistocchi R (2000) Metabolic responces of the diatom Achnanthes brevipes (Bacillariophycae) to nutrient limitation. J Phycol 36:882–890

    Article  CAS  Google Scholar 

  • Guzman-Murillo MA, Ascencio F (2000) Antiadhesive activity of sulfated polysaccharides of microalgae on the attachment of red sore disease-associated bacteria and Helicobacter pylori to tissue-culture cells. Lett Appl Microbiol 30:473–478

    Article  CAS  PubMed  Google Scholar 

  • Harun R, Danquah MK (2011) Influence of acid pre-treatment on microalgal biomass for bioethanol production. Process Biochem 46:304–309

    Google Scholar 

  • Haug A, Myklestad S (1976) Polysaccharides of marine diatoms with special reference to Chaetoceros species. Mar Biol 34:217–222

    Article  CAS  Google Scholar 

  • Hayashi K, Hayashi T, Kojima I (1996) A natural sulfated polysaccharide, calcium spirulan, isolated from Spirulina platensis: in vitro and ex vivo evaluation of anti-herpes simplex virus and anti-human immunodeficiency virus activities. AIDS Res Hum Retroviruses 12(15):1463–1471

    Article  CAS  PubMed  Google Scholar 

  • Helm RF, Huang Z, Edwards D, Leeson H, Peery W, Potts M (2000) Structural characterization of the released polysaccharide of desiccation-tolerant Nostoc commune DRH-1. J Bacteriol 182:974–982

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hernández-Corona A, Nieves I, Barron BL (2002) Antiviral activity of Spirulina maxima against herpes simplex virus type 2. Antiviral Res 56:279–285

    Article  PubMed  Google Scholar 

  • Hill DR, Peat A, Potts M (1994) Biochemistry and structure of the glycan secreted by desiccation-tolerant Nostoc commune (Cyanobacteria). Protoplasma 182:126–148

    Google Scholar 

  • Hill DR, Keenan TW, Helm RF, Potts M, Crowe LM, Crowe JH (1997) Extracellular polysaccharide of Nostoc commune (Cyanobacteria) inhibits fusion of membrane vesicles during desiccation. J Appl Phycol 9:237–248

    Google Scholar 

  • Hoagland KD, Rosowski JR, Gretz MR, Roemer SC (1993) Diatom extracellular polymeric substances: function, fine structure, chemistry, and physiology. J Phycol 29:537–566

    Article  CAS  Google Scholar 

  • Hoiczyck E, Hansel A (2000) Cyanobacterial cell walls: news from an unusual prokaryotic envelope. J Bacteriol 182:1191–1199

    Article  Google Scholar 

  • Hoiczyk E, Baumeister W (1998) The junctional pore complex, a prokaryotic secretion organelle, is the molecular motor underlying gliding motility in cyanobacteria. Curr Biol 8:1161–1168

    Article  CAS  PubMed  Google Scholar 

  • Hu Q (2004) Environmental effects on cell composition. In: Richmond A (ed) Handbook of microalgal culture: biotechnology and applied phycology. Blackwell Publishing, Oxford, pp 83–93

    Google Scholar 

  • Hu C, Liu Y, Paulsen BS, Petersen D, Klaveness D (2003) Extracellular carbohydrate polymers from five desert soil algae with different cohesion in the stabilization of fine sand grain. Carbohydr Polym 54:33–42

    Article  CAS  Google Scholar 

  • Huang G, Fang Q, Lechno-Yossef S, Wojciuch E, Wolk CP, Kaneko T, Tabata S (2005) Clustered genes required for the synthesis of the eterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Biotech 187:1114–1123

    CAS  Google Scholar 

  • Huang WJ, Lai CH, Cheng YL (2007) Evaluation of extracellular products and mutagenicity in cyanobacteria cultures separated from a eutrophic reservoir. Sci Total Environ 377:214–223

    Article  CAS  PubMed  Google Scholar 

  • Izumo A, Fujiwara S, Oyama Y, Satoh A, Fujita N, Nakamura Y, Tsuzuki M (2007) Physicochemical properties of starch in Chlorella change depending on the CO2 concentration during growth: comparison of structure and properties of pyrenoid and stroma starch. Plant Sci 172:1138–1147

    Article  CAS  Google Scholar 

  • Jensen S, Knutsen G (1993) Influence of light and temperature on photoinhibition of photosynthesis Spirulina platensis. J Appl Phycol 5:495–504

    Article  Google Scholar 

  • Ji C-F, Yu X-J, Chen Z-A, Xue S, Legrand J, Zhang W (2011) Effects of nutrient deprivation on biochemical compositions and photohydrogen production of Tetraselmis subcordiformis. Int J Hydrog Energy 36:5817–5821

    Article  CAS  Google Scholar 

  • Kataray SE, Dönmez G (2011) Microbial oil production from thermophile cyanobacteria for biodiesel production. Appl Energy 88:3632–3635

    Article  CAS  Google Scholar 

  • Kawaguchi T, Decho AW (2002) Isolation and biochemical characterization of extracellular polymeric secretions (eps) from modern soft marine stromatolites (Bahamas) and its inhibitory effect on CaCO3 precipitation. Prep Biochem Biotechnol 32:51–63

    Article  CAS  PubMed  Google Scholar 

  • Keidan M, Broshy H, van Moppes D, Arad Malis S (2006) Sulphur assimilation into the cell-wall polysaccharide of the red microalga Porphyridium sp. (Rhodophyta). Phycologia 45:505–511

    Google Scholar 

  • Keidan M, Friedlander M, Arad (Malis) S (2009) Effect of Brefeldin A on cell-wall polysaccharide production in the red microalga Phorphyridium sp. (Rhodophyta) through its effect on the Golgi apparatus. J Appl Phycol 21:707–717

    Article  CAS  Google Scholar 

  • Kiemle SN, Domozych DS, Gretz MR (2007) The extracellular polymeric substances of desmids (Conjugatophyceae, Streptophyta): chemistry, structural analyses and implications in wetland biofilms. Phycologia 46:617–627

    Article  Google Scholar 

  • Kumar AS, Mody K, Jha B (2007) Bacterial exopolysaccharides – a perception. J Basic Microbiol 47:103–117

    Article  CAS  PubMed  Google Scholar 

  • Lacombe-Harvey ME, Fukamizo T, Gragnon J, Ghinet MG, Denhart N, Letzel T, Brzezinski R (2009) Accessory active site residues of Streptomyces sp. N174 chitosanase: variations on an common theme in the lysozyme superfamily. FEBS J 276:857–869

    Article  CAS  PubMed  Google Scholar 

  • Lama L, Nicolaus B, Calandrelli V, Manca NC, Romano I, Gambacorta A (1996) Effect of growth conditiondions on endo- and exopolymer biosynthesis in Anabaena cylindrica 10C. Phytochem 42:655–659

    Article  CAS  Google Scholar 

  • Laurienzo P (2010) Marine polysaccharides in pharmaceutical applications: an overview. Mar Drugs 8:2435–2465

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li P, Harding SE, Liu Z (2001a) Cyanobacterial exopolysaccharides: their nature and potential biotechnological applications. Gen Eng Rev 18:375–404

    Article  CAS  Google Scholar 

  • Li S-Y, Lellouche J-P, Shabtai Y, Arad (Malis) S (2001b) Fixed carbon partitioning in the red microalga Phorphyridium sp. (Rhodophyta). J Phycol 37:289–297

    Article  CAS  Google Scholar 

  • Li H, Xu J, Liu Y, Ai S, Qin F, Li Z, Zhang H, Huang Z (2011) Antioxidant and moisture-retention activities of the polysaccharide from Nostoc commune. Carbohydr Polym 83:1821–1827

    Article  CAS  Google Scholar 

  • Lim CY, Yoo YH, Sidhartan M, Ma CW, Bang IC, Kim JM, Lee KS, Park NS, Shin HW (2006) Effects of copper(I) oxide on growth and biochemical compositions of two marine microalgae. J Environ Biol 27:461–466

    CAS  PubMed  Google Scholar 

  • Lopes DC, Maurer JBB, Stevan-Hancke FR, de Oliveira Proença LA, Zawadzki-Baggio SF (2012) Chemical analysis of exopolysaccharide fractions and lipid compounds of the microalga Heterosigma akashiwo grown in vitro. Bot Mar 55:565–580

    Google Scholar 

  • Lupescu N, Geresh S, Arad (Malis) S, Bernstein M, Glaser R (1991) Structure of the sulphated sugars isolated after acid hydrolysis of the extracellular polysaccharide of Phorphyridium sp. unicellular red alga. Carbohydr Res 210:349–352

    Article  CAS  Google Scholar 

  • Lupi FM, Fernandes HML, Sá -Correia I, Novais JM (1991) Temperature profiles of cellular growth and exopolysaccharide synthesis by Botryococus braunii Kütz. UC 58. J Appl Phycol 3:35–42

    Article  CAS  Google Scholar 

  • Magaletti E, Urbani R, Sist P, Ferrari CR, Cicero AM (2014) Abundance and chemical characterization of extracellular carbohydrates released by the marine diatom Cylindrotheca fusiformis under N- and P- limitation. Eur J Phycol 39:133–142

    Article  CAS  Google Scholar 

  • Maksimova IV, Bratkovskaya LB, Plekhanov SE (2004) Extracellular carbohydrates and polysaccharides of the alga Chlorella pyrenoidosa Chick S-39. Biol Bull 31:175–181

    Google Scholar 

  • Mann EE, Wozniak DJ (2012) Pseudomonas biofilm matrix composition and niche biology. FEMS Microbiol Rev 36:893–916

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Markou G, Angelidaki I, Georgakakis D (2012) Microalgal carbohydrates: an overview of the factors influencing carbohydrates production, and of main bioconversion technologies for production of biofuels. Appl Microbiol Biotechnol 96:631–645

    Article  CAS  PubMed  Google Scholar 

  • Marra M, Palmeri A, Ballio A, Segre A, Slodki ME (1990) Structural characterization of the exocellular polysaccharide from Cyanospira capsulata. Carbohydr Res 197:338–344

    Article  CAS  Google Scholar 

  • Matsunaga T, Sudo H, Takamesa H, Wachi Y, Nakamura N (1996) Sulfated extracellular polysaccharide production by the halophilic cyanobacterium Aphanocapsa halophytica immobilized on light-diffusing optical fibers. Appl Microbiol Biotechnol 45:24–27

    Article  CAS  Google Scholar 

  • Mazor G, Kidron GJ, Vonshak A, Abeliovich A (1996) The role of cyanobacterial exopolysaccharides in structuring desert microbial crusts. FEMS Microbiol Ecol 21:121–130

    Article  CAS  Google Scholar 

  • Megaletti E, Urbani R, Sist P, Ferrari CR, Cicero AM (2004) Abundance and chemical characterization of extracellular carbohydrates released by the marine diatom Cylindrotheca fusiformis under N-and P-limitation. Eur J Phycol 39(2):133–142

    Google Scholar 

  • Melis A (2007) Photosynthetic H2 metabolism in Chlamydomonas reinhardtii (unicellular green algae). Planta 226:1075–1086

    Article  CAS  PubMed  Google Scholar 

  • Micheletti E, Pereira S, Mannelli F, Moradas-Ferreira P, Tamagnini P, De Philippis R (2008) Sheathless mutant of cyanobacterium Gloeothece sp. Strain PCC 6909 with increased capacity to remove copper ions from aqueous solutions. Appl Environ Microbiol 74:2797–2804

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miklestad J, Haug A (1972) Production of carbohydrates by the marine diatom Chaetochos affinis and the effect of the concentration of nutrients in the culture medium. J Exp Mar Biol Ecol 9:125–136

    Article  Google Scholar 

  • Mishra A, Jha B (2009) Isolation and characterization of extracellular polymeric substances from micro-algae Dunaliella salina under salt stress. Bioresour Technol 100:3382–3386

    Article  CAS  PubMed  Google Scholar 

  • Mohammadi T, van Dam V, Sijbrandi R, Vernet T, Zapun A, Bouhss A, Diepeveen-de Bruin M, Nguyen-Distèche M, de Kruijff B, Breukink E (2011) Identification of FtsW as a transporter of lipid linked cell wall precursors across the membrane. EMBO J 30:1425–1432

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Moore BG, Tischer RG (1964) Extracellular polysaccharides of algae: effects on life-support systems. Science 145:586–587

    Article  CAS  PubMed  Google Scholar 

  • Moore BG, Tischer RG (1965) Biosynthesis of extracellular polysaccharides by the bleu-green alga Anabaena flos-aquae. Can J Microbiol 11:877–885

    Article  CAS  PubMed  Google Scholar 

  • Moreno J, Vargas MA, Madiedo JM, Muñoz J, Rivas J, Guerrero MG (2000) Chemical and rheological properties of an extracellular polysaccharide produced by the cyanobacterium Anabaena sp. ATCC 33047. Biotechnol Bioeng 67:283–290

    Article  CAS  PubMed  Google Scholar 

  • Morsi Abd-El-Monem H, Corradi MG, Gorbi G (1998) Toxicity of copper and zinc to two strains of Scenedesmus acutus having different sensitivity to chromium. Environ Exp Bot 40:59–66

    Article  Google Scholar 

  • Nakagawa M, Takamura Y, Yagi O (1987) Isolation and characterization of the slime from a cyanobacterium Microcystis aeruginosa K-3A. Agric Biol Chem 51:329–337

    Article  CAS  Google Scholar 

  • Nicolaus B, Panico A, Lama L, Ramano I, Manca MC, De Giulio A, Gambacorta A (1999) Chemical composition and production of exopolysaccharides from representative members of heterocystous and non-heterocystous cyanobacteria. Phytochemistry 52:639–647

    Article  CAS  Google Scholar 

  • Nie ZY, Xia JL, Levert JM (2002) Fractionation and characterization of polysaccharides from cyanobacterium Spirulina (Arthrospira) maxima in nitrogen-limited batch culture. J Cent South Univ Technol 9:81–86

    Article  CAS  Google Scholar 

  • Nielsen PH, Jahn A, Palmgren R (1997) Conceptual model for production and composition of exopolymers in biofilms. Water Sci Technol 36(1): 11–19

    Google Scholar 

  • Olivier B (2011) Hurdles and challenges for modelling and control of microalgae for CO2 mitigation and biofuel production. J Process Control 21(10):1378–1389

    Google Scholar 

  • Panoff JM, Priem B, Morvan H, Joset F (1988) Sulphated exopolysaccharides produced by two unicellular strains of cyanobacteria, Synechocystis PCC 6803 and 6714. Arch Microbiol 150:558–563

    Article  CAS  Google Scholar 

  • Parikh A, Madamwar D (2006) Partial characterization of extracellular polysaccharides from cyanobacteria. Bioresour Technol 97:1822–1827

    Article  CAS  PubMed  Google Scholar 

  • Patil JS, Anil AC (2005) Influence of diatom exopolymers and biofilms on metamorphosis in the barnacle Balanus amphitrite. Mar Ecol Prog Ser 301:231–245

    Article  CAS  Google Scholar 

  • Pereira S, Zille A, Micheletti E, Moradas-Ferreira P, De Philippis R, Tamagnini P (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33:917–941

    Article  CAS  PubMed  Google Scholar 

  • Pereira S, Mota R, Santos CL, De Philippis R, Tamagnini P (2013) Assembly and export of extracellular polymeric substances (EPS) in cyanobacteria: a phylogenomic approach. In: Chauvat F, Cassier – Chauvat C (eds) Genomics of cyanobacteria. Elsevier, Amsterdam, pp 235–279

    Chapter  Google Scholar 

  • Piorreck M, Baasch K-H, Pohl P (1984) Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and blue-green algae under different nitrogen regimes. Phytochemistry 23:207–216

    Article  CAS  Google Scholar 

  • Piro G, Congedo C, Leucci MR, Lenucci M, Dalessandro G (2005) The biosynthesis of exo- and cell wall-polysaccharides is sensitive to brefeldin A in the cyanobacterium Leptolyngbya VRUC 135. Plant Biosyst 139:107–112

    Article  Google Scholar 

  • Plesser L, Lapidot M, Weistein Y, Arad (Malis) S (2007) Sulfotransferase of red microalgae: a molecular and biochemical study. In: 8th Marine Biotechnology Conference. Eilat, p 31

    Google Scholar 

  • Plude JL, Parker DL, Schemmer OJ, Timmerman RJ, Hangstrom SA, Joers JM, Hnasko R (1991) Chemical characterization of the polysaccharide from the slime layer of the cyanobacterium Microcystis flos-aquae C3-40. Appl Environ Microbiol 57:1696–1700

    CAS  PubMed  PubMed Central  Google Scholar 

  • Potts M (1994) Desiccation tolerance of prokaryotes. Microbiol Rev 58:755–805

    CAS  PubMed  PubMed Central  Google Scholar 

  • Potts M (2001) Desiccation tolerance: a simple process? Trends Microbiol 9:553–559

    Article  CAS  PubMed  Google Scholar 

  • Pugh N, Ross SA, ElSohly HN, ElSohly MA, Pasco DS (2001) Isolation of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aquae and Chlorella pyrenoidosa. Planta Med 67:737–742

    Article  CAS  PubMed  Google Scholar 

  • Ramus J (1972) The production of extracellular polysaccharide by the unicellular red alga Phorphyridium aerugineum. J Phycol 8:97–111

    CAS  Google Scholar 

  • Raungsomboon S, Chidthaisong A, Bunnag B, Inthorn D, Harvey NW (2006) Production, composition and Pb2+ adsorption characteristics of capsular polysaccharides extracted from a cyanobacterium Gloeocapsa gelatinosa. Water Res 40:3759–3766

    Article  CAS  PubMed  Google Scholar 

  • Reddy KJ, Soper BW, Tang J, Bradley RL (1996) Phenotypic variation in exopolysaccharides production in the marine, aerobic nitrogen fixing unicellular cyanobacterium Cyanothece sp. World J Microbiol Biotechnol 12:311–318

    Article  CAS  PubMed  Google Scholar 

  • Reeves PR, Hobbs M, Valvano MA, Skurnik M, Whitfield C, Coplin D, Kido N, Klena J, Maskell D, Raetz CR, Rick PD (1996) Bacterial polysaccharide synthesis and gene nomenclature. Trends Microbiol 4:495–503

    Article  CAS  PubMed  Google Scholar 

  • Richert L, Golubic S, Le Guédès R, Ratiskol J, Payri C, Guezennec J (2005) Characterization of exopolysaccharides produced by cyanobacteria isolated from Polynesian microbial mats. Curr Microbiol 51:379–384

    Article  CAS  PubMed  Google Scholar 

  • Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112

    Article  CAS  PubMed  Google Scholar 

  • Rossi F, Micheletti E, Bruno L, Adhikary SP, Albertano P, De Philippis R (2012) Characteristics and role of the exocellular polysaccharides produced by five cyanobacteria isolated from phototrophic biofilms growing on Indian stone monuments. Biofouling 28:215–224

    Article  CAS  PubMed  Google Scholar 

  • Sangar VK, Dugan PR (1972) Polysaccharide produced by Anacystis nidulans: its ecological implication. Appl Microbiol 24:732–734

    CAS  PubMed  PubMed Central  Google Scholar 

  • Sassaki GL, Gorin PA, Reis RA, Serrato RV, Elífio SL, Iacomini M (2005) Carbohydrate, glycolipid and lipid components from the photobiont (Scytonema sp.) of the lichen, Dictyomema glabratum. Carbohydr Res 340:1808–1817

    Article  CAS  PubMed  Google Scholar 

  • Sassano CEN, Gioielli LA, Ferreira LS, Rodrigues MS, Sato S, Converti A, Carvalho JCM (2010) Evaluation of the composition of continuously-cultivated Arthrospira (Spirulina) platensis using ammonium chloride as nitrogen source. Biomass Bioenerg 34:1732–1738

    Article  CAS  Google Scholar 

  • Schultz T, Schumacher U, Prehm P (2007) Hyaluronan export by the ABC transporter MRP5 and its modulation by intracellular cGMP. J Biol Chem 282:20999–21004

    Article  CAS  Google Scholar 

  • Simon-Bercovitch B (1997) Cell wall formation in the red microalga Phorphyridium sp. PhD thesis, Ben – Gurion University of Negev, Israel

    Google Scholar 

  • Simon-Bercovitch B, Bar-Zvi D, Arad (Malis) S (1999) Cell wall formation during the cell cycle of Phorphyridium sp. (Rhodophyta). J Phycol 35:78–83

    Article  Google Scholar 

  • Sinnott ML (1990) Catalytic mechanisms of enzymatic glycosyl transfer. Chem Rev 90:1171–1202

    Article  CAS  Google Scholar 

  • Skorupska A, Janczarek M, Marczak M, Mazur A, Król J (2006) Rhizobial exopolysaccharides: genetic control and symbiotic functions. Microb Cell Fact 5:1–7

    Article  CAS  Google Scholar 

  • Soulat D, Jault JM, Geourjon C, Gouet P, Cozzone AJ, Grangeasse C (2007) Tyrosine-kinase Wzc from Escherichia coli possesses an ATPase activity regulated by autophosphorylation. FEMS Microbiol Lett 274:252–259

    Article  CAS  PubMed  Google Scholar 

  • Staats N, De Winder B, Stal L, Mur L (1999) Isolation and characterization of extracellular polysaccharides from the epipelic diatoms Cylindrotheca closterium and Navicula salinarum. Eur J Phycol 34:161–169

    Article  Google Scholar 

  • Steenbergen SM, Vimr ER (2008) Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment. Mol Biol 68:1252–1267

    CAS  Google Scholar 

  • Su C, Zhenming C, Lu W (2007) Optimization of medium and cultivation conditions for enhanced exopolysaccharide yield by marine Cyanothece sp. 113. Chin J Oceanol Limnol 25:411–417

    Article  CAS  Google Scholar 

  • Sudo H, Burgess JC, Takemasa H, Nakamura N, Matsunaga T (1995) Sulfated exopolysaccharides production by the halophilic cyanobacterium Aphanocapsa halophytia. Curr Microbiol 30:219–222

    Article  CAS  Google Scholar 

  • Sutherland IW (1982) Biosynthesis of microbial exopolysaccharides. Adv Microb Physiol 23:79–150

    Google Scholar 

  • Talyshinsky MM, Souprun YY, Huleihel MM (2002) Anti-viral activity of red microalgal polysaccharides against retroviruses. Cancer Cell Int 2:1–8

    Article  Google Scholar 

  • Tamaru Y, Takani Y, Yoshida T, Sakamoto T (2005) Crucial role of extracellular polysaccharides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune. Appl Environ Microbiol 71:7327–7333

    Google Scholar 

  • Tamiya H (1966) Synchronous cultures of algae. Annu Rev Plant Physiol 17:1–26

    Article  Google Scholar 

  • Tannin-Spitz T, Bergman M, van-Moppes D, Grossman S, Arad S (2005) Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J Appl Phycol 17:215–222

    Article  CAS  Google Scholar 

  • Tedesco M, Duerr E (1989) Light, temperature and nitrogen starvation effects on the total lipid and fatty acid content and composition of Spirulina platensis UTEX 1928. J Appl Phycol 1:201–209

    Article  Google Scholar 

  • Tesson B, Hildebrand M (2013) Characterization and location of insoluble organic matrices associated with diatom cell walls: insight into their roles during cell wall formation. PLoS One 8(4):e61675

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thornton DCO, Dong LF, Underwood GJC, Nedwell DB (2002) Factors affecting microphytobentic biomass, species composition and production in the Colne estuary (UK). Aquat Microb Ecol 27:285–300

    Article  Google Scholar 

  • Tischer RG, Davis EB (1971) The effect of various nitrogen sources upon the production of extracellular polysaccharide by the blue-green alga Anabaena flos-aquae A-37. J Exp Bot 22:546–551

    Article  CAS  Google Scholar 

  • Ucko M, Geresh S, Simon-Bercovitch S, Arad (Malis) S (1994) Predation by a dinoflagellate on a red microalga with cell wall modified by sulfate and nitrate starvation. Mar Ecol Prog Ser 104:293–298

    Article  Google Scholar 

  • Ulvskov P, Soares Paiva S, Domozych D, Harholt J (2013) Classification, naming and evolutionary history of glycosyltransferases from sequenced green and red algal genomes. PLoS One 8(10):e76511

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Urbani R, Sist P, Pletikapić G, Mišić Radić T, Svetlićić V, Žutić V (2012) Diatom polysaccharides: extracellular production, isolation and molecular characterization. In: Karunaratn DN (ed) Complex world of polysaccharide. Intech, Rijeka, pp 346–356

    Google Scholar 

  • Vincenzini M, De Philippis R, Sili C, Materassi R (1990) Studies on exopolysaccharide release by diazotrophic batch cultures of Cyanospira capsulata. Appl Microbiol Biotechnol 34:392–396

    Article  CAS  Google Scholar 

  • Vogel PV, Garvin DF, Mockler TC, Schmutz J, Rokhsar D, Bevan MV (2010) Genome sequencing and and analysis of the model grass Brachipodium distachyon. Nature 463:763–768

    Article  CAS  Google Scholar 

  • Volk RB, Venzke K, Blaschek W (2007) Structural investigation of a polysaccharide released by the cyanobacterium Nostoc insulare. J Appl Phycol 19:255–262

    Article  CAS  Google Scholar 

  • Vonshak A (2002) Spirulina platensis (Arthrospira): physiology, cell biology and biotechnology. Taylor & Francis, London

    Google Scholar 

  • Wang WS, Tischer RG (1973) Study of the extracellular polysaccharide produced by a blue-green alga Anabaena flos-aquae A-37. Arch Microbiol 91:77–81

    CAS  Google Scholar 

  • Wang Y, Lu J, Mollet J-C, Gretz MR, Hoagland KD (1997) Extracellular matrix assembly in diatoms (Bacillariophyceae). 11. 2,6-Dichlorobenzonitrile inhibition of motility and stalk production in the marine diatom Achnanthes longipes. Plant Physiol 113:1071–1080

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Whitfield C (2006) Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Ann Rev Biochem 75:39–68

    Article  CAS  PubMed  Google Scholar 

  • Whitfield C (2010) Glycan chain-length control. Nat Chem Biol 6:403–404

    Article  CAS  PubMed  Google Scholar 

  • Whitfield C, Larue K (2008) Stop and go: regulation of chain length in the biosynthesis of bacterial polysaccharides. Nat Struct Mol Biol 15:121–123

    Article  CAS  PubMed  Google Scholar 

  • Whitfield C, Paiment A (2003) Biosynthesis and assembly of group 1 capsular polysaccharides in Escherichia coli and related extracellular polysaccharides in other bacteria. Carbohydr Res 338:2491–2502

    Article  CAS  PubMed  Google Scholar 

  • Whitfield C, Roberts IS (1999) Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol Microbiol 31:1307–1319

    Article  CAS  PubMed  Google Scholar 

  • Witvrouw M, De Clercq E (1997) Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen Pharmacol 29:497–511

    Article  CAS  PubMed  Google Scholar 

  • Wotton RS (2004) The ubiquity and many roles of exopolymers (EPS) in aquatic systems. Sci Mar 68:13–21

    Article  CAS  Google Scholar 

  • Wustman BA, Gretz MR, Hoagland KD (1997) Extracellular matrix assembly in diatoms (Bacillariophyceae). 1. A model of adhesives based on chemical characterization and localization of polysaccharides from the marine datom Achnanthes longipes and other diatoms. Plant Physiol 113:1059–1069

    CAS  PubMed  PubMed Central  Google Scholar 

  • Xin L, Hu HY, Ke G, Sun YX (2010) Effects of different nitrogen and phosphorus concentrations on the growth, nutrient uptake, and lipid accumulation of a freshwater microalga Scenedesmus sp. Bioresour Technol 101:5494–5500

    Article  CAS  PubMed  Google Scholar 

  • Xu Y, Rossi F, Colica G, Deng S, De Philippis R, Chen L (2013) Use of cyanobacterial polysaccharides to promote shrub performances in desert soils: a potential approach for the restoration of desertified areas. Biol Fertil Soils 49:143–152

    Article  CAS  Google Scholar 

  • Yoshimura H, Okamoto S, Tsumuraya Y, Ohmori M (2007) Group 3 sigma factor gene, sigJ, a key regulator of desiccation tolerance, regulates the synthesis of extracellular polysaccharide in cyanobacterium Anabaena sp. strain PCC 7120. DNA Res 14:13–24

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yoshimura H, Kotake T, Aohara T, Tsumuraya Y, Ikeuchi M, Ohmori M (2012) The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp. strain HK-01 in NaCl tolerance. J Appl Phycol 24:237–243

    Article  CAS  Google Scholar 

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Rossi, F., De Philippis, R. (2016). Exocellular Polysaccharides in Microalgae and Cyanobacteria: Chemical Features, Role and Enzymes and Genes Involved in Their Biosynthesis. In: Borowitzka, M., Beardall, J., Raven, J. (eds) The Physiology of Microalgae. Developments in Applied Phycology, vol 6. Springer, Cham. https://doi.org/10.1007/978-3-319-24945-2_21

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