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Regulation of Membrane Na+-K+ ATPase pp 63–75Cite as

Calcium Controls the P2-ATPase Mediated Homeostasis: Essential Role of NaAF

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Part of the book series: Advances in Biochemistry in Health and Disease ((ABHD,volume 15))

Abstract

This chapter reveals a new unique story of Ca-signaling in upholding cellular homeostasis. The cytosolic activator (regulatory) protein, NaAF (of 170 kDa mass), for the ubiquitous P2-ATPase (and 80 kDa HAF solely for the gastric H/K-ATPase) is essential for P2-ATPase function. The NaAF and HAF function as the allosteric operator-cum gate-keeper of the dual channel P2-ATPase system (where mirror-image orientation of the two α-subunits serves as the membrane-embedded in-and-out gates) for simultaneous transport of two ions. The entire cyclic operation is in turn fine-tuned by local Ca (μM) as top (allosteric) controller of the P2-ATPase to maintain homeostasis. Thus at lower range Ca (<2) stimulates, but at higher range (>2) Ca abruptly inhibits the HAF-stimulated H/K-ATPase abolishing it at 4 μM Ca. At this point the (K ± HAF)-independent basal (Mg-dependent) activity of the H/K-ATPase acts as a provisional Ca-ATPase pump in an altered state to remove excess Ca, thus resuming the initial Ca-activated HAF-regulated state of a new cycle. Identical Ca-signaling operations also control the universal NaAF-regulated Na/K-ATPase system.

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Acknowledgements

The work was supported in part by the USPHS grant, AM19531, and RCDA (Research Career Development Award), AM 00623, from the NIH and Surgical Research Fund of SUNY-Upstate Medical Center, NY 13210. The author is currently involved in mind-body Research with Vivekananda Mind-Body Research Institute (VMBRI) associated with the Ramakrishna-Sarada Vedanta Center of Phoenix, Tempe, AZ 85281

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Authors and Affiliations

  1. SUNY-Upstate Medical Center, Syracuse, NY, 13210, USA

    Tushar Ray

  2. Ramakrishna-Sarada Vedanta Center of Phoenix, Phoenix, AZ, 85281, USA

    Tushar Ray

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  1. Tushar Ray
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Correspondence to Tushar Ray .

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Editors and Affiliations

  1. Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, India

    Sajal Chakraborti

  2. University of Manitoba, Institute of Cardiovascular Science St. Boniface Hospital Research, Winnipeg, Manitoba, Canada

    Naranjan S Dhalla

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Ray, T. (2016). Calcium Controls the P2-ATPase Mediated Homeostasis: Essential Role of NaAF. In: Chakraborti, S., Dhalla, N. (eds) Regulation of Membrane Na+-K+ ATPase. Advances in Biochemistry in Health and Disease, vol 15. Springer, Cham. https://doi.org/10.1007/978-3-319-24750-2_4

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