Abstract
The first eukaryotic replication origin was isolated from S. cerevisiae in the late 1970s using a plasmid maintenance assay. Combined with Sanger sequencing, this assay gave valuable insights into origin structure in S. cerevisiae and a few other yeast species. Fast-forward to this millennium, and the same simple assay in conjunction with modern “next-generation” sequencing and other high-throughput techniques testing origin structure and activity has led to an explosion of powerful approaches for dissecting origin sequence and function. Although such studies are still in their infancy, they have already uncovered a surprising diversification of origin sequences over a relatively short evolutionary time span. In this chapter we focus on how these approaches are being applied to understand origin structure and evolution in diverse species of budding yeasts. These approaches hold out the hope that through a comprehensive analysis of origin function across the budding yeast lineage, we can begin to understand the evolutionary forces that shape the replication landscape.
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Acknowledgments
We thank Bonny Brewer for critical reading of this manuscript. I.L. was supported by a grant from the NSF (1243710). M.K.R. was supported by grants from the NIH (GM018926) and the NSF (1243710).
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Raghuraman, M.K., Liachko, I. (2016). Sequence Determinants of Yeast Replication Origins. In: Kaplan, D. (eds) The Initiation of DNA Replication in Eukaryotes. Springer, Cham. https://doi.org/10.1007/978-3-319-24696-3_7
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