Advanced Protein Expression Using Bombyx mori Nucleopolyhedrovirus (BmNPV) Bacmid in Silkworm

  • Tatsuya Kato
  • Enoch Y. ParkEmail author
Part of the Entomology in Focus book series (ENFO, volume 4)


An expression system using a Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid has been developed and used for efficient expression of protein using silkworm. Silkworm can sustain large-scale production of recombinant proteins due to its ease of rearing and scaling-up. Our chapter focuses on the modification of a BmNPV bacmid for a more efficient protein expression system. For example, we discuss how to achieve construction of a stronger promoter, less proteolytic degradation of expressed proteins, and a chaperone-coexpressed expression system. We describe the application of functional BmNPV particles purified from silkworm hemolymph to vaccines, antibody production, and transmembrane protein analysis. For human use, the major problem of proteins produced in silkworm is contamination by adventitious agents and protein quality. Of special concern is that N-glycosylation in silkworms is of a high-mannose type in most cases, which is different from the complex type found in mammals. We end by looking to future prospects for integration/applications of protein expression systems with silkworm biotechnology.


Recombinant Protein Rabies Virus Classical Swine Fever Virus Recombinant Protein Production Recombinant Protein Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Human α1,4-N-acetylglucosaminyltransferase


Autographa californica multiple nucleopolyhedrovirus


Antibody-dependent cell-mediated cytotoxicity


Human β1,3-N-acetylglucosaminyltransferase


Human heavy chain-binding protein


Bombyx mori


Bombyx mori nucleopolyhedrovirus


Cysteine protease-deficient BmNPV

BmNPV CP Chi (CPChi)

Cysteine protease- and chitinase-deficient BmNPV


Human calnexin


Human calreticulin


Classical swine fever virus


Drug delivery system


Enzyme-linked immunosorbent assay


Human ERp57


Foot-and-mouth disease virus


Green fluorescent protein




Heat shock protein 70


Immunoglobulin G




Large unilamellar vesicle


Middle silk gland

PIT, 7d

Post-injection time, 7 days


Posterior silk gland


Rous sarcoma virus


Rabies virus


Surface antigen 1


Sulforhodamine B


Surface antigen 1-related sequence 2


α2,6-sialyltransferase 1


Virus-like particle


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Green Chemistry Research Division, Research Institute of Science and TechnologyShizuoka UniversityShizuokaJapan

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