Bioanalytical SERS Applications

  • Marek ProchazkaEmail author
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)


SERS spectroscopy can be applied for the detection of biologically relevant molecules in real complex matrix (e.g. human body fluids, drinks, food). The quantitative SERS measurements are, in practice, the result of considerable effort in optimizing an enhancing substrate and experimental conditions. This chapter will explain the basic principles of quantitative bioanalytical SERS measurements from the point of view of SERS-active substrates, internal intensity standards, sensitivity (limit of detection) and specificity. The successful SERS sensing of analytes with very low affinity for SERS-enhancing surface is possible due to suitable chemical modification of a metal surface to promote the capture of particular analytes. The results of some SERS bioanalytical applications on pharmaceuticals, drugs (including nicotine and cocaine), pollutants and pesticides, food contaminants and food additives (melamine, food colourants) and biowarfare agents (anthrax) will be summarized. The limits of detection as well as accuracy of SERS analysis are comparable and in some cases even better than these provided by standard analytic techniques (such as HPLC).


SERS Spectrum SERS Signal SERS Substrate SERS Intensity SERS Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Institute of PhysicsCharles University in PraguePragueCzech Republic

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