Abstract
Angiogenesis, with a prominent role in tumor growth, is a physiological process through which new blood vessels are generated from pre-existing vasculature. The effect of angiogenesis inhibition test agents to cell differentiation can be evaluated using the matrigel differentiation assay, an environment simulating in vitro the natural angiogenic process under study. Using this assay, angiogenic activity can be evaluated by the quantification of tubules, junctions and other structures of interest in the matrigel images, i.e. microphotographs of the formed cellular networks under different fields of view. In this work, we present an image analysis workflow that uses separate channels in the RGB matrigel images, each contributing different information related to angiogenic activity (tubules, junctions) and other structures (cells, lumens). The workflow is successfully showcased on the basis of a series of matrigel images generated for the evaluation of angiogenesis inhibition compounds, and results are cross-validated with a manual expert scoring.
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Potente, M., Gerhardt, H., Carmeliet, P.: Basic and Therapeutic Aspects of Angiogenesis. Cell 146(6), 873–887 (2011)
Folkman, J.: Isolation of a Tumor Factor Responsible for Angiogenesis. J. Exp. Med. 133, 275–288 (1971)
Bridges, E.M., Harris, A.L.: The Angiogenic Process as a Therapeutic Target in Cancer. Biochem. Pharmacol. 81(10), 1183–1191 (2011)
Schmidt, T., Carmeliet, P.: Angiogenesis: a target in solid tumors, also in leukemia? Hematology Am. Soc. Hematol. Educ. Program. 2011, 1–8 (2011). doi:10.1182/asheducation-2011.1.1
El-Kenawi, A.E., El-Remessy, A.B.: Angiogenesis inhibitors in cancer therapy: mechanistic perspective on classification and treatment rationales. Br. J. Pharmacol. 170(4), 712–729 (2013). doi:10.1111/bph.12344
Hughes, C.S., Postovit, L.M., Lajoie, G.A.: Matrigel: a complex protein mixture required for optimal growth of cell culture. Proteomics 10(9), 1886–1890 (2010). doi:10.1002/pmic.200900758
Ko, J.M., Lung, M.L.: In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay. Bio-protocol 2(18), e260 (2012). http://www.bio-protocol.org/e260
Doukas, C.N., Maglogiannis, I., Chatziioannou, A.A.: Computer-supported angiogenesis quantification using image analysis and statistical averaging. IEEE Trans. Inf. Technol. Biomed. 12(5), 650–657 (2008). doi:10.1109/TITB.2008.926463
Khoo, C.P., Micklem, K., Watt, S.M.: A comparison of methods for quantifying angiogenesis in the Matrigel assay in vitro. Tissue Eng. Part C Methods 17(9), 895–906 (2011). doi:10.1089/ten.TEC.2011.0150
Gallo-Oller, G., Rey, J.A., Dotor, J., Castresana, J.S.: Quantitative method for in vitro matrigel invasiveness measurement through image analysis software. Mol. Biol. Rep. 41(10), 6335–6341 (2014). doi:10.1007/s11033-014-3556-0
Donovan, D., Brown, N.J., Bishop, E.T., Lewis, C.E.: Comparison of three in vitro human ‘angiogenesis’ assays with capillaries formed in vivo. Angiogenesis 4(2), 113–121 (2001)
Michailidou, M., Brown, H.K., Lefley, D.V., Evans, A., Cross, S.S., Coleman, R.E., Brown, N.J., Holen, I.: Microvascular Endothelial Cell Responses in vitro and in vivo: Modulation by Zoledronic Acid and Paclitaxel? J. Vasc. Res. 47(6), 481–493 (2010)
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Valavanis, I., Goudas, T., Michailidou, M., Maglogiannis, I., Loutrari, H., Chatziioannou, A. (2015). A Novel Image Analysis Methodology for the Evaluation of Angiogenesis in Matrigel Assays and Screening of Angiogenesis-Modulating Compounds. In: Chbeir, R., Manolopoulos, Y., Maglogiannis, I., Alhajj, R. (eds) Artificial Intelligence Applications and Innovations. AIAI 2015. IFIP Advances in Information and Communication Technology, vol 458. Springer, Cham. https://doi.org/10.1007/978-3-319-23868-5_5
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