Skip to main content
  • 753 Accesses

Abstract

Flow cytometric analysis is a powerful tool to determine the lineage, phenotype, and other characteristics of hematopoietic cells. It uses the principles of light scattering and light excitation and the emission of fluorochrome molecules to generate specific multiparameter data from particles and cells. It is a highly sensitive technique that can quantify the expression of eight or more colors/markers on a single cell and therefore enables the identification of aberrant cells within a complex cellular background.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Suggested Readings

  1. Caraway NP. Strategies to diagnose lymphoproliferative disorders by fine-needle aspiration by using ancillary studies. Cancer. 2005;105:432–42.

    Article  PubMed  Google Scholar 

  2. Chen HI, Akpolat I, Mody DR, Lopez-Terrada D, De Leon AP, Luo Y, Jorgensen J, Schwartz MR, Chang CC. Restricted kappa/lambda light chain ratio by flow cytometry in germinal center B cells in Hashimoto thyroiditis. Am J Clin Pathol. 2006;125:42–8.

    Article  PubMed  Google Scholar 

  3. Chen YH, Gong Y. Cytopathology in the diagnosis of lymphoma. In: Nayar R, editor. Cytology in oncology. New York: Springer; 2013. p. 211–40.

    Google Scholar 

  4. Gong Y. Ancillary studies on neoplastic cytologic specimens. In: Nayar R, editor. Cytology in oncology. New York: Springer; 2013. p. 13–29.

    Google Scholar 

  5. Gong Y, Caraway N, Gu J, Zaidi T, Fernandez R, Sun X, Huh YO, Katz RL. Evaluation of interphase fluorescence in situ hybridization for the t(14;18)(q32;q21) translocation in the diagnosis of follicular lymphoma on fine-needle aspirates: a comparison with flow cytometry immunophenotyping. Cancer. 2003;99:385–93.

    Article  PubMed  Google Scholar 

  6. Jorgensen JL. State of the art symposium: flow cytometry in the diagnosis of lymphoproliferative disorders by fine-needle aspiration. Cancer. 2005;105:443–51.

    Article  PubMed  Google Scholar 

  7. Katz RL RL. Cytologic diagnosis of leukemia and lymphoma. Values and limitations. Clin Lab Med. 1991;11:469–99.

    PubMed  Google Scholar 

  8. Katz RL, Hirsch-Ginsberg C, Childs C, Dekmezian R, Fanning T, Ordonez N, Cabanillis F, Sneige N. The role of gene rearrangements for antigen receptors in the diagnosis of lymphoma obtained by fine-needle aspiration. A study of 63 cases with concomitant immunophenotyping. Am J Clin Pathol. 1991;96:479–90.

    CAS  PubMed  Google Scholar 

  9. Kussick SJ, Kalnoski M, Braziel RM, Wood BL. Prominent clonal B-cell populations identified by flow cytometry in histologically reactive lymphoid proliferations. Am J Clin Pathol. 2004;121:464–72.

    Article  PubMed  Google Scholar 

  10. Verstovsek G, Chakraborty S, Ramzy I, Jorgensen JL. Large B-cell lymphomas: fine-needle aspiration plays an important role in initial diagnosis of cases which are falsely negative by flow cytometry. Diagn Cytopathol. 2002;27:282–5.

    Article  PubMed  Google Scholar 

  11. Wang J, Katz RL, Stewart J, Landon G, Guo M, Gong Y. Fine-needle aspiration diagnosis of lymphomas with signet ring cell features: potential pitfalls and solutions. Cancer Cytopathol. 2013;121:525–32.

    Article  PubMed  Google Scholar 

  12. Zhao X, Gong Y. Fine needle aspiration diagnosis of an early-onset post-transplant lymphoproliferative disorder. Diagn Cytopathol. 2011;39:788–90.

    Article  PubMed  Google Scholar 

  13. Zhao XF, Cherian S, Sargent R, Seethala R, Bonner H, Greenberg B, Bagg A. Expanded populations of surface membrane immunoglobulin light chain-negative B cells in lymph nodes are not always indicative of B-cell lymphoma. Am J Clin Pathol. 2005;124:143–50.

    Article  PubMed  Google Scholar 

  14. Zhang S, Gong Y. From cytomorphology to molecular pathology: maximizing the value of cytology of lymphoproliferative disorders and soft tissue tumors. Am J Clin Pathol. 2013;140:454–67.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yun Gong .

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Gong, Y. (2016). Flow Cytometric Immunophenotyping. In: Metastatic Neoplasms in Fine-Needle Aspiration Cytology. Springer, Cham. https://doi.org/10.1007/978-3-319-23621-6_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-23621-6_5

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-23620-9

  • Online ISBN: 978-3-319-23621-6

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics