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Substrate Interaction Networks of the Escherichia coli Chaperones: Trigger Factor, DnaK and GroEL

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Prokaryotic Systems Biology

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 883))

Abstract

In the dense cellular environment, protein misfolding and inter-molecular protein aggregation compete with protein folding. Chaperones associate with proteins to prevent misfolding and to assist in folding to the native state. In Escherichia coli, the chaperones trigger factor, DnaK/DnaJ/GrpE, and GroEL/ES are the major chaperones responsible for insuring proper de novo protein folding. With multitudes of proteins produced by the bacterium, the chaperones have to be selective for their substrates. Yet, chaperone selectivity cannot be too specific. Recent biochemical and high-throughput studies have provided important insights highlighting the strategies used by chaperones in maintaining proteostasis in the cell. Here, we discuss the substrate networks and cooperation among these protein folding chaperones.

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Acknowledgements

Vaibhav Bhandari was the recipient of a Jaro Sodek Award—Ontario Student Opportunity Trust Fund (OSOTF) fellowship from the Department of Biochemistry at the University of Toronto. This work was supported by a grant from the Canadian Institutes of Health Research (MOP-130374) to WAH.

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Authors and Affiliations

  1. Department of Biochemistry, University of Toronto, 1 King’s College Circle, Medical Sciences Building, Room 5308, Toronto, ON, M5S 1A8, Canada

    Vaibhav Bhandari & Walid A. Houry

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  1. Vaibhav Bhandari
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  2. Walid A. Houry
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Correspondence to Walid A. Houry .

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  1. Cellular and Molecular Pharmacology, Univ of California, San Francisco, SAN FRANCISCO, California, USA

    Nevan J. Krogan, PhD

  2. Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada

    Mohan Babu, PhD

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Bhandari, V., Houry, W.A. (2015). Substrate Interaction Networks of the Escherichia coli Chaperones: Trigger Factor, DnaK and GroEL. In: Krogan, PhD, N., Babu, PhD, M. (eds) Prokaryotic Systems Biology. Advances in Experimental Medicine and Biology, vol 883. Springer, Cham. https://doi.org/10.1007/978-3-319-23603-2_15

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