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Vegetative Incompatibility in Botrytis

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Botrytis – the Fungus, the Pathogen and its Management in Agricultural Systems

Abstract

In ascomycetes, vegetative compatibility is determined by a series of het or vic genes that exist in two or more allelic states. Strains that carry identical alleles at all loci are compatible; those that differ at one or more loci are incompatible. The genetic basis of vegetative incompatibility in Botrytis cinerea is not known, but is presumed conform to the system found in other ascomycetes. Using nitrate non-utilizing mutants, 66 distinct vegetative compatibility groups (VCGs) were identified among field and single ascospore isolates of B. cinerea, an observation that is consistent with the presence of at least six het genes. Sulphate non-utilizing mutants have also been used to identify multiple VCGs in this species. The large number of VCGs and the limited occurrence of isolates displaying the same VCG suggest that sexual recombination plays an important role in field populations of B. cinerea. Mycelial compatibility groups (MCGs) recognized by the formation of interaction lines between isolates paired on agar media (barrages), indicate incompatibility in B. cinerea, however, there is no direct correlation with VCGs recognized by complementation. Sexual crosses have been shown to generate new B. cinerea VCGs. Unlike the situation in Neurospora crassa, in B. cinerea, the MAT1 locus does not act as a het gene. The homologue of the N. crassa het-c gene has been cloned and sequenced in B. cinerea, but it does not act as a vic gene in B. cinerea.

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Authors and Affiliations

  1. Department of Plant Pathology and Weed Research, Agricultural Research Organization, The Volcani Center, Bet Dagan, 50250, Israel

    Nadia Korolev & Yigal Elad

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  1. Nadia Korolev
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  2. Yigal Elad
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Correspondence to Nadia Korolev .

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Editors and Affiliations

  1. Centre de Versailles-Grignon, INRA, Thiverval-Grignon, France

    Sabine Fillinger

  2. Dept. Plant Path. and Weed Res., ARO, Bet Dagan, Israel

    Yigal Elad

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Korolev, N., Elad, Y. (2016). Vegetative Incompatibility in Botrytis . In: Fillinger, S., Elad, Y. (eds) Botrytis – the Fungus, the Pathogen and its Management in Agricultural Systems. Springer, Cham. https://doi.org/10.1007/978-3-319-23371-0_4

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