Abstract
This chapter presents in detail how digital microfluidic biochips work, and introduces the architecture model we use in the book. Digital microfluidic biochips are organized as an array of electrodes, each of which can hold one droplet, and move the droplets of fluid using electrokinetics. We present the key ideas behind electrowetting-on-dielectric, the fluid propulsion method used in these biochips. We discuss the basic microfluidic operations, such as transport, splitting, dispensing, mixing, and detection, focusing on the reconfigurable operations, which are characteristic to droplet-based biochips. The reconfigurable operations are typically performed inside “virtual modules”, which are created by grouping adjacent cells. During module-based operation execution, all cells inside the module are considered occupied, although the droplet uses only one cell at a time, which is inefficient. Therefore, we introduce a new, “routing-based”, model of operation execution and propose an analytical method for determining the completion time of an operation on any given route. The chapter also presents the typical faults affecting digital microfluidic biochips and the fault models considered in this book, as well as a detailed discussion of how these faults can affect the operation execution.
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Notes
- 1.
Electrode pitch size = 1.5 mm, gap spacing = 0.3 mm, average linear velocity = 20 cm/s.
References
Chakrabarty, K., Zeng, J.: Design Automation Methods and Tools for Microfluidics-Based Biochips. Springer, Dordrecht (2006)
Chakrabarty, K., Fair, R.B., Zeng, J.: Design tools for digital microfluidic biochips: towards functional diversification and more than Moore. Trans. Comput. Aided Des. Integr. Circuits Syst. 29(7), 1001–1017 (2010). doi:10.1109/TCAD.2010.2049153
Dhar, S., Drezdon, S., Maftei, E.: Digital microfluidic biochip for malaria detection. Technical report, Duke University (2008)
Fan, S.-K., Hashi. C., Kim, C.-J.: Manipulation of multiple droplets on N × M grid by cross-reference EWOD driving scheme and pressure-contact packaging. In: Proceedings of the International Conference on MEMS, pp. 694–697 (2003)
Fair, R.B., Srinivasan, V., Paik, P., Pamula, V.K., Pollack, M.G.: Electrowetting-based on-chip sample processing for integrated microfluidics. In: IEEE International Electron Devices Meeting, pp. 779–782 (2003)
Fair, R.B., Khlystov, A., Tailor, T.D., Ivanov, V., Evans, R.D., Srinivasan, V., Pamula, V.K., Pollack, M.G., Griffin, P.B., Zhou, J.: Chemical and biological applications of digital-microfluidic devices. IEEE Des. Test Comput. 24(1), 10–24 (2007). doi:http://dx.doi.org/10.1109/MDT.2007.8
Gong, M., Kim, C.J.: Two-dimensional digital microfluidic system by multilayer printed circuit board. In: Proceedings of the Conference on Micro Electro Mechanical Systems, pp. 726–729 (2005)
Gong, J., Fan, S.K., Kim, C.J., et al.: Portable digital microfluidics platform with active but disposable lab-on-chip. In: Proceedings of the 17th IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 355–358 (2004)
Ho, T.Y., Zeng, J., Chakrabarty, K.: Digital microfluidic biochips: a vision for functional diversity and more than Moore. In: Proceedings of the International Conference on Computer-Aided Design, pp. 578–585 (2010)
Hu, K., Hsu, B.N., Madison, A., Chakrabarty, K., Fair, R.B.: Fault detection, real-time error recovery, and experimental demonstration for digital microfluidic biochips. In: Proceedings of the Conference on Design, Automation and Test in Europe, pp. 559–564 (2013)
Hwang, W., Su, F., Chakrabarty, K.: Automated design of pin-constrained digital microfluidic arrays for lab-on-a-chip applications. In: Proceedings of the Design Automation Conference, pp. 925–930 (2006)
Luo, Y., Chakrabarty, K., Ho, T.Y.: Error recovery in cyberphysical digital microfluidic biochips. IEEE Trans. Comput. Aided Des. Integr. Circuits Syst. 32(1), 59–72 (2013)
Luo, Y., Chakrabarty, K., Ho, T.Y.: Real-time error recovery in cyberphysical digital-microfluidic biochips using a compact dictionary. IEEE Trans. Comput. Aided Des. Integr. Circuits Syst. 32(12), 1839–1852 (2013)
Maftei, E., Pop, P., Madsen, J.: Routing-based synthesis of digital microfluidic biochips. Des. Autom. Embed. Syst. 16(1), 19–44 (2012)
Maftei, E., Pop, P., Madsen, J.: Module-based synthesis of digital microfluidic biochips with droplet-aware operation execution. J. Emerg. Technol. Comput. Syst. 9(1), 2 (2013)
Mark, D., Haeberle, S., Roth, G., von Stetten, F., Zengerle, R.: Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem. Soc. Rev. 39(3), 1153–1182 (2010)
Miller, E., Wheeler, A.R.: Digital bioanalysis. Anal. Bioanal. Chem. 393(2), 419–426 (2009)
Moon, H., Wheeler, A.R., Garrell, R.L., Loo, J.A., Kim, C.J.: An integrated digital microfluidic chip for multiplexed proteomic sample preparation and analysis by MALDI-MS. Lab Chip 6(9), 1213–1219 (2006)
Mukhopadhyay, R.: Microfluidics: on the slope of enlightenment. Anal. Chem. 81(11), 4169–4173 (2009)
Paik, P., Pamula, V.K., Fair, R.B.: Rapid droplet mixers for digital microfluidic systems. Lab Chip 3, 253–259 (2003)
Pollack, M.G., Shenderov, A.D., Fair, R.B.: Electrowetting-based actuation of droplets for integrated microfluidics. Lab Chip 2, 96–101 (2002)
Ren, H., Fair, R.B.: Micro/nano liter droplet formation and dispensing by capacitance metering and electrowetting actuation. In: Proceedings of the IEEE-NANO, pp. 369–372 (2002)
Ren, H., Srinivasan, V., Fair, R.B.: Design and testing of an interpolating mixing architecture for electrowetting-based droplet-on-chip chemical dilution. In: Proceedings of the International Conference on Transducers, Solid-State Sensors, Actuators and Microsystems, pp. 619–622 (2003)
Srinivasan, V., Pamula, V.K., Pollack, M., Fair, R.B.: A digital microfluidic biosensor for multianalyte detection. In: Proceedings of the Micro Electro Mechanical Systems Conference, pp. 327–330 (2003)
Srinivasan, V., Pamula, V.K., Fair, R.B.: Droplet-based microfluidic lab-on-a-chip for glucose detection. Anal. Chim. Acta 507, 145–150 (2004)
Srinivasan, V., Pamula, V.K., Fair, R.B.: An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. Lab Chip 4, 310–315 (2004)
Su, F., Ozev, S., Chakrabarty, K.: Concurrent testing of droplet-based microfluidic systems for multiplexed biomedical systems. In: Proceedings of the International Test Conference, pp. 883–892 (2004)
Su, F., Ozev, S., Chakrabarty, K.: Ensuring the operational health of droplet-based microelectrofluidic biosensor systems. IEEE J. Sens. 5, 763–773 (2005)
Su, F., Hwang, W., Mukherjee, A., Chakrabarty, K.: Testing and diagnosis of realistic defects in digital microfluidic biochips. J. Electron. Test. 23(2–3), 219–233 (2007)
Tabeling, P.: Introduction to Microfluidics. Oxford University Press, Oxford (2006)
Xu, T., Chakrabarty, K.: Functional testing of digital microfluidic biochips. In: Proceedings of the International Test Conference, pp. 1–10 (2007)
Xu, T., Chakrabarty, K.: Parallel scan-like test and multiple-defect diagnosis for digital microfluidic biochips. Trans. Biomed. Circuits Syst. 1(2), 148–158 (2007)
Xu, T., Chakrabarty, K.: Broadcast electrode-addressing for pin-constrained multi-functional digital microfluidic biochips. In: Proceedings of the Design Automation Conference, pp. 173–178 (2008)
Xu, T., Chakrabarty, K.: Fault modeling and functional test methods for digital microfluidic biochips. Trans. Biomed. Circuits Syst. 3(4), 241–253 (2009)
Zhao, Y., Chakrabarty, K.: Cross-contamination avoidance for droplet routing. In: Design and Testing of Digital Microfluidic Biochips, pp. 27–55. Springer, New York (2013)
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Pop, P., Alistar, M., Stuart, E., Madsen, J. (2016). Biochip Architecture Model. In: Fault-Tolerant Digital Microfluidic Biochips. Springer, Cham. https://doi.org/10.1007/978-3-319-23072-6_3
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DOI: https://doi.org/10.1007/978-3-319-23072-6_3
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