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Cytopreparatory Techniques

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The Paris System for Reporting Urinary Cytology

Abstract

Nomenclature reporting systems for exfoliative cytopathology must include a description of specimen collection and processing for these are the first steps in the diagnostic process and influence all the steps downstream. Regardless of their technical details, collection and preparation methods must allow for the adequate presentation of cellular findings for diagnostic interpretation. First, efforts must be made to harvest fresh viable cells with minimal degeneration. Second, the cells must be concentrated and transferred from a fluid suspension onto a clean glass surface in a way that allows the cells to adhere and flatten in a predictable way. Third, the preparation must be fixed to retain cellular features. Finally, the slide with the cells on it must be stained and mounted. Each of these steps can be done in a variety of ways, but without an explicit acknowledgement and validation of each step, quality control of the diagnostic process is sure to be elusive.

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Author information

Authors and Affiliations

  1. Cyto-Logics, Inc., Indianapolis, IN, USA

    Gary W. Gill C.T. (A.S.C.P.)

  2. Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indiana University Health Pathology Laboratory, 350 West 11th Street, Room 6002, Indianapolis, IN, USA

    William N. Crabtree Ph.D., S.C.T. (A.S.C.P.)

  3. Department of Pathology, Johns Hopkins Hospital, Baltimore, MD, USA

    Deidra P. Kelly C.T. (A.S.C.P.)

Authors
  1. Gary W. Gill C.T. (A.S.C.P.)
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  2. William N. Crabtree Ph.D., S.C.T. (A.S.C.P.)
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  3. Deidra P. Kelly C.T. (A.S.C.P.)
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Corresponding author

Correspondence to William N. Crabtree Ph.D., S.C.T. (A.S.C.P.) .

Editor information

Editors and Affiliations

  1. The Johns Hopkins Hospital, The Johns Hopkins University, Baltimore, Maryland, USA

    Dorothy L. Rosenthal

  2. Department of Pathology, Loyola University Medical Center Department of Pathology, MAYWOOD, Illinois, USA

    Eva M. Wojcik

  3. Department of Pathology and Laboratory M, University of Wisconsin School of Medine Department of Pathology and Laboratory M, MADISON, Wisconsin, USA

    Daniel F.I. Kurtycz

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© 2016 Springer International Publishing Switzerland

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Gill, G.W., Crabtree, W.N., Kelly, D.P. (2016). Cytopreparatory Techniques. In: Rosenthal, D., Wojcik, E., Kurtycz, D. (eds) The Paris System for Reporting Urinary Cytology. Springer, Cham. https://doi.org/10.1007/978-3-319-22864-8_10

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  • DOI: https://doi.org/10.1007/978-3-319-22864-8_10

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-22863-1

  • Online ISBN: 978-3-319-22864-8

  • eBook Packages: MedicineMedicine (R0)

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