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Carbon Nanomaterials for Biomedical Applications pp 533–542Cite as

Fluorescent Nanosensor for Drug Discovery

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Part of the book series: Springer Series in Biomaterials Science and Engineering ((SSBSE,volume 5))

Abstract

There is a pressing need for developing new anti-HIV agents due to the emergence of drug-resistant HIV mutants, side effects of existing drugs, and the mutation of the virus. High-throughput screening (HTS) has been proven as a powerful technique for the discovery of new anti-HIV drugs. The utilization of high-content screening (HCS) requires development of nanosensor that is suitable for HCS. We developed fluorescence imaging-based nanosensor for screening of inhibitors against activity of HIV-1 protease. We explored using AcGFP1 (a fluorescent mutant of the wild-type green fluorescent protein) and mCherry (a mutant of red fluorescent protein), as two fluorophores for Förster resonance energy transfer (FRET) microscopy imaging measurement of HIV-1 protease activity within living cells. Both in vitro and in vivo studies revealed that the novel molecular probes exhibit significant enhancement of FRET signals. The probe developed in this study enables HCS of new anti-HIV agents.

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Author information

Authors and Affiliations

  1. Department of Biomedical Engineering, College of Engineering, University of Arkansas, 72701, Fayetteville, AR, USA

    Sha Jin, Huantong Yao & Erika Ellis

Authors
  1. Sha Jin
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  2. Huantong Yao
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  3. Erika Ellis
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Corresponding author

Correspondence to Sha Jin .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA

    Mei Zhang

  2. Soft Matter Materials Branch, Air Force Research Laboratory, Dayton, Ohio, USA

    Rajesh R. Naik

  3. Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio, USA

    Liming Dai

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© 2016 Springer International Publishing Switzerland

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Jin, S., Yao, H., Ellis, E. (2016). Fluorescent Nanosensor for Drug Discovery. In: Zhang, M., Naik, R., Dai, L. (eds) Carbon Nanomaterials for Biomedical Applications. Springer Series in Biomaterials Science and Engineering, vol 5. Springer, Cham. https://doi.org/10.1007/978-3-319-22861-7_17

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