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Recasting the Local and the Global: The Three Lives of Protein Sequencing in Spanish Biomedical Research (1967–1995)

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The Local Configuration of New Research Fields

Part of the book series: Sociology of the Sciences Yearbook ((SOSC,volume 29))

Abstract

This chapter explores how protein sequencing – a technique enabling the determination of the fine structure of protein chains – circulated across different biomedical fields in Spain during the last third of the twentieth century. By focusing on three individual scientists, I argue that protein sequencing had three distinct Spanish lives and was inextricably linked to the biographies of its users. Between 1967 and 1995, protein sequencing shifted from an aid to prevent agrarian plagues in Franco’s dictatorship to a promising diagnostic tool in the transition towards democracy and, finally, an out-of-fashion technique overshadowed by the emergence of recombinant DNA methods. My proposed three lives challenge over-simplistic comparative frameworks and suggest that the local configuration of a new scientific technique should not be sought in its similarities or idiosyncratic differences with a given ‘global’. Protein sequencing in Spain was rather shaped by the researchers’ will of constructing a professional space of their own and achieving a complex equilibrium between the fulfilment of local demands and the engagement with what was considered international and cutting-edge at each historical time.

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Notes

  1. 1.

    As two out of my three subject scientists are deceased, I reconstructed each life by combining uncatalogued personal and institutional archives, published scientific literature and oral histories with relatives and colleagues.

  2. 2.

    On the scientific component of the Development Plans, see reports of the Comisión Asesora de Investigación Científica y Técnica (Madrid, President’s Office, 1964–1986). On the possibility to create a “protected space” for scientific research and the national variations of generic governance, see Gläser, Laudel and Lettkemann (Chap. 2).

  3. 3.

    For Martín-Municio’s correspondence with EMBO and syllabi of the first editions of the course see Personal Archive of A. Martín-Municio, Faculty of Chemistry, Universidad Complutense de Madrid (Spain), uncatalogued file on EMBO.

  4. 4.

    For a discussion of a related case (how Chagas disease and the associated parasite ‘Trypanosoma cruzi’ have been addressed as a scientific problem), see Kreimer (Chap. 10).

  5. 5.

    Personal Archive of A. Martín-Municio, Faculty of Chemistry, Universidad Complutense de Madrid (Spain), uncatalogued file on NSF. From the late-1950s onwards, the US Administration had seen in Franco’s regime a potential ally against Communism, which had resulted in institutions devoted to cultural, educational and scientific exchange. This collaboration boosted the international diplomacy of the dictatorship and was complemented with other initiatives focused on the European front, such as the Spanish membership of EMBO or its previous integration in CERN.

  6. 6.

    R. Manso, J. Ávila, M. Salas and A. Sánchez, interviews with author, Universidad Autónoma de Madrid and Central Headquarters of CSIC, 2009, 2010 and 2011.

  7. 7.

    B. Frangione, phone interview with author, 2009, and personal communication, 2012. Annual reports of the Department of Pathology, NYU Medical Center, Archives of New York University, 1963–1973.

  8. 8.

    On the importance of place in emerging scientific spaces see Meyer and Molyneux-Hodgson (Chap. 4).

  9. 9.

    Personal Archive of A. Martín-Municio, Faculty of Chemistry, Universidad Complutense de Madrid (Spain), uncatalogued folder on NSF. J. Gavilanes and R. Rodríguez, interview with author, Faculty of Chemistry, Universidad Complutense de Madrid, 2009 and 2012.

  10. 10.

    CBM, Memoria, 1975–1978. F. Soriano, interview with author, Centro de Ciencias Humanas y Sociales (CSIC), 2011.

  11. 11.

    Newspaper Archive of the Spanish Biblioteca Nacional, June 2011. Search of keywords ‘Hospital Ramón y Cajal’ with chronological restriction 1965–1985.

  12. 12.

    E. Rodríguez Ocaña and T. Ortiz: “Medical research in Franco’s Spain: an overview”, paper delivered at the conference Science, Scientists and Totalitarian Systems: Spanish Science during Francoism, Universidad Pompeu Fabra, Barcelona, 2008. J.M. Sancho Rof, interview with author, Hospital Ramón y Cajal, 2010. Hospital Ramón y Cajal, Memoria Anual, years 1977–1979.

  13. 13.

    On the importance of specialised techniques and training spaces see Sormani (Chap. 13).

  14. 14.

    Hospital Ramón y Cajal, Memoria Anual, years 1980–1994. In 1992, the format of the reports changed and Méndez’s laboratory features only under the section “Research Activity”.

  15. 15.

    Quote from Ley 13/1986, de 14 de abril, de Fomento y Coordinación General de la Investigación Científica y Técnica, section “Exposición de motivos”. Available at http://www.boe.es/buscar/doc.php?id=BOE-A-1986-9479 (last accessed March 2014).

  16. 16.

    CBM, Memoria, 1981–1985.

  17. 17.

    C. López-Otín, J. Ávila and M. Salas, interviews with author, phone and CBM, 2008 and 2009.

References

  • Abelson, J. 1980. A revolution in biology. Science 209: 1319–1321.

    Article  Google Scholar 

  • Abir-Am, P. 1987. The biotheoretical gathering, trans-disciplinary authority and the incipient legitimation of molecular biology in the 1930s: New perspective on the historical sociology of science. History of Science 25: 1–70.

    Article  Google Scholar 

  • Camprubí, L. 2010. One grain, one nation: Rice genetics and the corporate state in early Francoist Spain (1939–1952). Historical Studies in the Natural Sciences 40: 499–531.

    Article  Google Scholar 

  • De Chadarevian, S. 1996. Sequences, conformation, information: Biochemists and molecular biologists in the 1950s. Journal of the History of Biology 29: 361–386.

    Article  Google Scholar 

  • De Chadarevian, S., and J.-P. Gaudillière. 1996. The tools of the discipline: Biochemists and molecular biologists. Journal of the History of Biology 29: 327–330.

    Article  Google Scholar 

  • De Chadarevian, S., and B. Strasser. 2002. Molecular biology in postwar Europe: Towards a ‘glocal’ picture. Studies in History and Philosophy of Biological and Biomedical Sciences 33: 361–365.

    Article  Google Scholar 

  • Fernández-Sousa, J.M., J.G. Gavilanes, A.M. Municio, J.A. Paredes, A. Pérez-Aranda, and R. Rodriguez. 1975. Primary structure of cytochrome c from the insect Ceratitis capitata. Biochimica et Biophysica Acta (BBA) – Protein Structure 393: 358–367.

    Article  Google Scholar 

  • Freije, J., S. Laín, E. Viñuela, and C. López Otín. 1993. Nucleotide sequence of a nucleoside triphosphate phosphohydrolase gene from African swine fever virus. Virus Research 30: 63–72.

    Article  Google Scholar 

  • García-Sancho, M. 2010. A new insight into Sanger’s development of sequencing: From proteins to DNA, 1943-1977. Journal of the History of Biology 43: 265–323.

    Article  Google Scholar 

  • García-Sancho, M. 2012. Biology, computing and the history of molecular sequencing: From proteins to DNA (1945-2000). Basingstoke: Palgrave-Macmillan.

    Book  Google Scholar 

  • Gavilanes, J., M.A. Lizarbe, A.M. Municio, M. Olmo, and M. Onaderra. 1982. Biología molecular del colágeno. Colágeno del insecto ceratitis capitata. Revista de la Real Academia de Ciencias Exactas Físicas y Naturales 76: 719–750.

    Google Scholar 

  • Gavilanes, J., C. López Otín, F. Gavilanes, and E. Méndez. 1984. Conformational studies of the human complex-forming glycoprotein, heterogeneous in charge: Protein HC. Biochemistry 23: 1234–1238.

    Article  Google Scholar 

  • Gavroglu, K. 1999. The sciences in the European periphery during the enlightenment. Dordrecht: Kluwer Academic Publishers.

    Book  Google Scholar 

  • Gomez Rodriguez, A., and A. Canales Serrano. 2009. The rebels and the new Spanish scientific culture. Journal of War and Culture Studies 2: 321–333.

    Article  Google Scholar 

  • Harwood, J. 1993. Styles of scientific thought: The German genetics community. Chicago: University of Chicago Press.

    Google Scholar 

  • Howlett, P., and M. Morgan (eds.). 2010. How well do facts travel? Cambridge: Cambridge University Press.

    Google Scholar 

  • Jordan, K., and M. Lynch. 1998. The dissemination, standardization and routinization of a molecular biological technique. Social Studies of Science 28: 773–800.

    Article  Google Scholar 

  • López Otín, C., J. Freije, F. Parra, E. Méndez, and E. Viñuela. 1990. Mapping and sequence of the gene coding for protein p72, the major capsid protein of African swine fever virus. Virology 175: 477–484.

    Article  Google Scholar 

  • Martín-Municio, A. 1969. Proyección biológica de los lípidos. Madrid: Real Academia de Ciencias Exactas, Físicas y Naturales.

    Google Scholar 

  • Mellado, R., E. Méndez, E. Viñuela, and M. Salas. 1977. Order of the two major head protein genes of bacteriophage phi 29 of Bacillus subtilis. Journal of Virology 24: 378–382.

    Google Scholar 

  • Méndez, E., and J. Gavilanes. 1975. Fluorometric detection of peptides after column chromatography or on paper: o-phthalaldehyde and fluorescamine. Analytical Biochemistry 72: 473–479.

    Article  Google Scholar 

  • Méndez, E., B. Frangione, and S. Kochwa. 1973. Chemical typing of human immunoglobulins E and D. FEBS Letters 33: 4–6.

    Article  Google Scholar 

  • Muñoz, E., and J. Sebastián. 2008. Exploración de la política científica en España: de la espeleología a la cartografía. In Cien años de política científica en España, ed. M.J. Santesmases and A. Romero de Pablos, 357–384. Madrid: Fundación BBVA.

    Google Scholar 

  • Nieto-Galán, A. 1998. The images of science in modern Spain. Rethinking the “polemica”. In The sciences in the European periphery during the enlightenment, ed. K. Gavroglu, 65–86. Dordrecht: Kluwer Academic Publishers.

    Google Scholar 

  • Ophir, A., and S. Shapin. 1991. The place of knowledge a methodological survey. Science in Context 4: 3–22.

    Google Scholar 

  • Ortuño, J. 2003. Notas contra la amnesia en su primer cuarto de siglo. In XXV Aniversario Hospital Ramón y Cajal, ed. V. Authors, 13–33. Madrid: Editores Médicos.

    Google Scholar 

  • Pairolí, M. 1996. Joan Oró. Barcelona: Fundació Catalana per a la Recerca.

    Google Scholar 

  • Papanelopoulou, F., A. Nieto-Galán, and E. Perdiguero (eds.). 2008. Popularizing science and technology in the European periphery, 1800-2000. Surrey: Ashgate Publishers.

    Google Scholar 

  • Rodríguez, R., M.A. Lizarbe, and J. Gavilanes. 1990. El árbol de las proteínas y otros relatos científicos. In Departamento de Bioquímica: Profesor Ángel Martín-Municio (1966-1989), ed. J. Gavilanes, 83–95. Madrid: Universidad Complutense de Madrid.

    Google Scholar 

  • Ryle, A.P., F. Sanger, L.F. Smith, and R. Kitai. 1955. The disulphide bonds of insulin. Biochemical Journal 60: 541–556.

    Article  Google Scholar 

  • Salas, M. 2007. 40 years with bacteriophage Phi29. Annual Review of Microbiology 61: 1–22.

    Article  Google Scholar 

  • Santesmases, M.J. 2000. Severo Ochoa and the biomedical sciences in Spain under Franco, 1959-1975. Isis 91: 706–734.

    Article  Google Scholar 

  • Santesmases, M.J. 2002. National politics and international trends: EMBO and the making of molecular biology in Spain (1960-1975). Studies in History and Philosophy of Biological and Biomedical Sciences 33: 473–487.

    Article  Google Scholar 

  • Santesmases, M. 2006. Peace propaganda and biomedical experimentation: Influential uses of radioisotopes in endocrinology and molecular genetics in Spain (1947–1971). Journal of the History of Biology 39: 765–794.

    Article  Google Scholar 

  • Santesmases, M.J. 2013. Cereals, chromosomes and colchicine: Crop varieties at the Estación Experimental Aula Dei and human cytogenetics, 1948-1958. In Human heredity in the twentieth century, ed. B. Gausemeier, S. Müller-Wille, and E. Ramsden, 127–140. London: Pickering and Chatto.

    Google Scholar 

  • Santesmases, M.J., and C. Gradmann. 2011. Circulation of antibiotics: An introduction. Dynamis 31: 293–303.

    Article  Google Scholar 

  • Saraiva, T., and N. Wise. 2010. Autarky/Autarchy: Genetics, food production, and the building of Fascism. Historical Studies in the Natural Sciences 40: 419–428.

    Article  Google Scholar 

  • Schaffer, S., and S. Shapin. 1985. Leviathan and the air pump: Hobbes, boyle and the experimental life. New Jersey: Princeton University Press.

    Google Scholar 

  • Secord, J.A. 2004. Knowledge in transit. Isis 95: 654–672.

    Article  Google Scholar 

  • Shapin, S., and A. Thackray. 1974. Prosopography as a research tool in history of science: The British scientific community 1700-1900. History of Science 12: 1–28.

    Article  Google Scholar 

  • Strasser, B. 2010. Collecting, comparing, and computing sequences: the making of Margaret O. Dayhoff’s “Atlas of protein sequence and structure”, 1954-1965. Journal of the History of Biology 43: 623–660.

    Article  Google Scholar 

  • Suárez-Díaz, E., and A. Barahona. 2013. Post-war and post-revolution: Medical genetics and social anthropology in Mexico, 1945-70. In Human heredity in the twentieth century, ed. B. Gausemeier, S. Müller-Wille, and E. Ramsden, 101–112. London: Pickering and Chatto.

    Google Scholar 

  • Subrahmanyam, S. 1997. Connected histories: Notes towards a reconfiguration of early modern Eurasia. Modern Asian Studies 31: 735–762.

    Article  Google Scholar 

  • Werner, M., and B. Zimmermann. 2006. Beyond comparison: histoire croisée and the challenge of reflexivity. History and Theory 45: 30–50.

    Article  Google Scholar 

  • Wright, S. 1994. Molecular politics: Developing American and British regulatory policy for genetic engineering, 1972-1982. Chicago: University of Chicago Press.

    Google Scholar 

  • Yáñez, R., J. Rodríguez, M. Nogal, L. Yuste, C. Enríquez, J. Rodríguez, and E. Viñuela. 1995. Analysis of complete nucleotide sequence of African swine fever virus. Virology 208: 249–278.

    Article  Google Scholar 

  • Yi, D. 2008. Cancer, viruses and mass migration: Paul Berg’s venture into eukaryotic biology and the advent of recombinant DNA research and technology, 1967-1980. Journal of the History of Biology 41: 589–636.

    Article  Google Scholar 

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Acknowledgements

Special thanks are given to M.J. Santesmases, A. Romero, E. Muñoz and other researchers at the Instituto de Filosofía (CSIC), as well as to the editors of this volume. J. Baines thoroughly polished my English prior to publication. The scientists I interviewed – and their PAs – kindly accommodated me in their busy schedules. One of them, A. Sánchez Álvarez-Insúa, died shortly after the interview and this chapter is a tribute to his memory. E. Méndez’s widow, Pilar, provided both valuable memories and records. Other people who helped me at different stages of my work are: X. Calvó, A. Nieto-Galán, A. Presas, J. Guillem, J.R. Bertomeu, D. Teira, J. Bunde, A. Lee, R. Gutiérrez, V. Olmedo and P. Martínez. The research for this chapter was funded by the projects HUM2006-04939/FISO, FFI2009-07522 and FFI2012-34076, awarded by the Spanish National R&D Programme, and by two contracts within the schemes JAE-Doc and Juan de la Cierva, awarded by CSIC and the Spanish Government.

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García-Sancho, M. (2016). Recasting the Local and the Global: The Three Lives of Protein Sequencing in Spanish Biomedical Research (1967–1995). In: Merz, M., Sormani, P. (eds) The Local Configuration of New Research Fields. Sociology of the Sciences Yearbook, vol 29. Springer, Cham. https://doi.org/10.1007/978-3-319-22683-5_12

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