Abstract
Recent incorporation of new molecular technologies, for example genomics and molecular markers, to the conventional plant breeding methods has resulted in a paradigm shift in crop improvement strategies. Proteomics is a comparatively new tool which is gradually becoming essential for the new generation of plant breeders because it is capable of providing new insights at cellular levels. Proteome, a translational version of a genome, is vital to expose molecular mechanisms essential for plant growth, development, and their interactions with physical and biological environment. A specific advantage of proteomics over all other -omics methods in crop breeding advancement is its ability to consider post-translational modifications that reflect the functional impressions of protein modifications on crop plant productivity. In this chapter we discuss various proteomic techniques, with examples of their applications for the advancement of conventional crop breeding programs. Proteomic methods can be used to measure subtle changes in protein expression levels in response to selective breeding, and for biotic and abiotic stress tolerance studies among different germplasm or cultivars. We demonstrate that the prospects of possible inclusion of proteomic techniques will lead to stronger crop breeding programs and a solid food security in the coming years.
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The authors acknowledge financial support from USDA/SunGrant, SD Agriculture Experiment Station, SD Wheat Commission and SD Soybean Research and Promotion Council.
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Das, A., Paudel, B., Rohila, J.S. (2015). Potentials of Proteomics in Crop Breeding. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools. Springer, Cham. https://doi.org/10.1007/978-3-319-22521-0_18
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