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Highly Accurate 3D Shape and Deformation Measurements Using Fluorescent Stereo Microscopy

  • Conference paper
Advancement of Optical Methods in Experimental Mechanics, Volume 3

Abstract

Biomechanics has been developing at rapid pace in recent decades. For investigation of the biotissues, biomaterials or biofilms under the microscale or the nanoscale, it urgently demands an accurate measurement technique for three-dimensional (3D) surface profilometry and deformation in real-time. Traditional stereo microscope with stereo-based digital image correlation (DIC) works well on common materials, but it is hard to apply to moisture sample due to the specular reflections which could cause large decorrelation among those stereo images. In this paper, we described a fluorescent stereo microscopy (FSM) measurement method for surface profilometry and deformation based on stereo-based DIC. Due to the complex lens combination of a microscope, the distortion of the optical system is hard to formulate accurately using ordinary distortion models. Thus, it could cause large reconstruction errors, particularly in Z-direction in height. In order to improve the accuracy, a new distortion correction scheme is introduced along with a new calibration board. This distortion correction method is intended for use prior to stereo-vision calibration by mapping sensor coordinates of generic image coordinates to a virtual ideal plane. In order to demonstrate this technique, a sequence pair of images of a biofilm is captured during growth, and the 3D surface profilometry and deformation was measured with high accuracy accordingly. A detailed description of this technique is presented in this paper.

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Acknowledgements

HL acknowledges the support of ONR MURI 0014-11-1-0691, AFOSR FA9550-14-1-0227, US Army W91CBR-13-C-0037, DOE NEUP 09-416, and NSF ECCS-1459044 and CMMI-1031829. HL also thanks the Louis A. Beecherl Chair for additional support.

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Authors and Affiliations

  1. TWI Ltd. Granta Park, Great Abington, Cambridge, CB21 6AL, UK

    Zhenxing Hu

  2. Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX, 75080, USA

    Zhenxing Hu, Huiyang Luo & Hongbing Lu

Authors
  1. Zhenxing Hu
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  2. Huiyang Luo
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  3. Hongbing Lu
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Corresponding author

Correspondence to Hongbing Lu .

Editor information

Editors and Affiliations

  1. Sandia National Laboratories, Livermore, USA

    Helena Jin

  2. Department of Chemistry and Physics, Southeastern Louisiana University, Hammond, Louisiana, USA

    Sanichiro Yoshida

  3. Dipartimento Meccanica Matematica e Management, Politecnico Di Bari, Bari, Italy

    Luciano Lamberti

  4. National Chung Hsing University, Graduate Institute of Precision Eng., Taiwan, China

    Ming-Tzer Lin

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Hu, Z., Luo, H., Lu, H. (2016). Highly Accurate 3D Shape and Deformation Measurements Using Fluorescent Stereo Microscopy. In: Jin, H., Yoshida, S., Lamberti, L., Lin, MT. (eds) Advancement of Optical Methods in Experimental Mechanics, Volume 3. Conference Proceedings of the Society for Experimental Mechanics Series. Springer, Cham. https://doi.org/10.1007/978-3-319-22446-6_11

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  • DOI: https://doi.org/10.1007/978-3-319-22446-6_11

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-22445-9

  • Online ISBN: 978-3-319-22446-6

  • eBook Packages: EngineeringEngineering (R0)

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