Abstract
Genomic characterization is a complex science that started several decades before the setup of microarrays. Nevertheless, due to the increasing need to characterize all genetic changes, and thanks to technical development such as improvement of throughput, miniaturization, computational biology, etc., a huge variety of DNA microarrays were set up in the 2000s. The use of microarray for translational studies has demonstrated their benefit to highlight genomic abnormalities for multiple diseases such as cancers. Whole genome analysis has demonstrated the genome complexity based on the type of alteration, their size, and their frequencies of tumor cells among normal cells. Genomic instability and subclonal identification of tumors were also other properties that can characterize for broad tumors (sarcomas, breast cancers, etc.). The development of high-content microarrays and the use of polymorphic probe (SNP) helped biologist and bioinformaticians to design pipelines to identify and characterize genomic alterations for several sources of specimen.
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Acknowledgments
Thanks to Cécile Reyes, Emilie Henry, and Audrey Rapinat for providing illustrations of samples they analyzed for the genomic core facility. Thanks to Dr. Gudrun Schleiermacher, MD PhD, and Chicard Mathieu for their collaboration on ctDNA.
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Gentien, D., Reyes, C. (2015). Microarrays-Based Molecular Profiling to Identify Genomic Alterations. In: Le Tourneau, C., Kamal, M. (eds) Pan-cancer Integrative Molecular Portrait Towards a New Paradigm in Precision Medicine. Springer, Cham. https://doi.org/10.1007/978-3-319-22189-2_4
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DOI: https://doi.org/10.1007/978-3-319-22189-2_4
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