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Bacterial Diversity in Clay and Actinide Interactions with Bacterial Isolates in Relation to Nuclear Waste Disposal

  • Chapter
Radionuclides in the Environment

Abstract

One potential source of actinides (An) in the environment could be the accidental release from nuclear waste disposal sites. Hence, the long-term safety of nuclear waste in a deep geologic repository is an important issue in our society. Microorganisms indigenous to potential host rocks are able to influence the speciation and therefore the mobility of An and their retardation both by direct and indirect pathways. They can as well affect the conditions in a geologic repository (e.g., by gas generation or canister corrosion). The focus of this chapter lies on the influence of indigenous microbes on the speciation of An. Therefore, for the safety assessment of such a repository, it is necessary to know which microorganisms are present in the potential host rocks (clay and salt) and if these microorganisms can influence the speciation of released An. Hence, dominant bacterial strains from potential host rocks for future nuclear waste depositions have to be investigated regarding their interaction mechanisms with soluble An ions. This chapter will cover the following research areas. Gained knowledge concerning the bacterial diversity in, e.g., Mont Terri Opalinus clay by applying direct molecular culture-independent retrievals and cultivation experiments will be presented. Their influence on the geochemical behavior of selected An (uranium and curium) will be highlighted. These investigations contribute to a better understanding of microbial interactions of An on a molecular level for an improved prediction of the safety of a planned nuclear waste repository.

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Acknowledgments

The authors thank the BMWi for financial support (contract nos.: 02E10618 and 02E10971), Velina Bachvarova and Sonja Selenska-Pobell for microbial diversity analysis and isolation of the bacteria, Monika Dudek for cultivation of the bacteria, as well as the BGR for providing the clay samples. The authors are indebted to the US Department of Energy, Office of Basic Energy Sciences, for the use of 248Cm via the transplutonium element production facilities at Oak Ridge National Laboratory; 248Cm was made available as part of collaboration between HZDR and the Lawrence Berkeley National Laboratory (LBNL).

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  1. Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstraße 400, 01328, Dresden, Germany

    Henry Moll & Andrea Cherkouk

  2. Institut für Radioökologie und Strahlenschutz (IRS), Leibniz Universität Hannover, 30419, Hannover, Germany

    Laura Lütke

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  1. Henry Moll
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Correspondence to Henry Moll .

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  1. Institut für Radioökologie und Strahlens, Gottfried Wilhelm Leibniz Universität Ha, Hannover, Germany

    Clemens Walther

  2. Institut für Radioökologie und Strahlens, Gottfried Wilhelm Leibniz Universität Ha, Hannover, Germany

    Dharmendra K. Gupta

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Moll, H., Lütke, L., Cherkouk, A. (2015). Bacterial Diversity in Clay and Actinide Interactions with Bacterial Isolates in Relation to Nuclear Waste Disposal. In: Walther, C., Gupta, D. (eds) Radionuclides in the Environment. Springer, Cham. https://doi.org/10.1007/978-3-319-22171-7_12

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