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Theoretical Aspects

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Book cover Introduction to Fluorescence Sensing

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Abstract

Quantitative measures are highly needed in fluorescence sensing. In this Chapter the reader finds discussion on parameters that have to be optimized in every sensor, such as selectivity, sensitivity and limit of detection. Concentrating on reversible binding, in which the mass action law is observed, the methods of determining the binding constants and modeling the ligand binding isotherms are presented. Kinetics of target binding and its influence on the results of analyte determination are discussed. Focusing on fluorescence sensing, linear and intensity-weighted formats are distinguished and analyzed. At the end of this Chapter the reader finds the Section “Sensing and thinking” with the list of questions and problems. It addresses the issue, how to provide the optimal quantitative measure of target binding.

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Authors and Affiliations

  1. Laboratory of Nanobiotechnology Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Alexander P. Demchenko

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  1. Alexander P. Demchenko
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© 2015 Springer International Publishing Switzerland

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Demchenko, A.P. (2015). Theoretical Aspects. In: Introduction to Fluorescence Sensing. Springer, Cham. https://doi.org/10.1007/978-3-319-20780-3_2

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