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Detection and Distribution of Sphingolipids in Tissue by FTICR MALDI-Imaging Mass Spectrometry

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Book cover Bioactive Sphingolipids in Cancer Biology and Therapy

Abstract

MALDI imaging mass spectrometry is an evolving technology capable of simultaneously profiling multiple analytes of interest across a tissue section and aligning their distribution to tissue histopathology. This chapter summarizes a MALDI imaging workflow for on-tissue identification of sphingolipids and glycosphingolipids using tissues derived from mouse models known to accumulate ceramides (Farber disease) and globotriaosylceramides (Fabry disease). A combination of CID and on-tissue enzyme digestions was utilized for structural confirmation prior to being added to the comprehensive sphingolipid and glycosphingolipid library. Two example case studies related to the modulation of sphingolipid metabolism are provided to illustrate the potential applications of MALDI imaging. In the first case study, distinct ceramides were visualized in relation to Lewis lung carcinoma tumors. In the second case study, tissues were derived from a tumor xenograft model treated with a drug targeting the sphingosine-1-phosphate/ceramide nexus. Representative images of ceramide and hexose ceramides in relation to cancer alone or drug distribution correlating to an increase or decrease in sphingosine-1-phosphate or ceramide species are provided. These MALDI imaging workflows can be readily adapted to assess the distribution of sphingolipids and glycosphingolipids in any tissue system of interest.

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Author information

Authors and Affiliations

  1. Department of Cell and Molecular Pharmacology, MUSC Proteomics Center and Hollings Cancer Center, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC, 29425, USA

    E. Ellen Jones & Richard R. Drake

  2. Institute of Medical Science, University of Toronto, 67 College Street, Toronto, ON, M5G 2M1, Canada

    Shaalee Dworski & Jeffrey A. Medin

  3. University Health Network, 67 College Street, Toronto, ON, M5G 2M1, Canada

    Mustafa Kamani & Jeffrey A. Medin

  4. Departments of Medicine and Biochemistry and the Stony Brook Cancer Center, Stony Brook University Medical Center, 101 Nicholls Road, Stony Brook, NY, 11794, USA

    Daniel Canals & Masayuki Wada

  5. Department of Microbiology and Immunology and Hollings Cancer Center, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC, 29425, USA

    Xiang Liu & James Norris

  6. Stony Brook Cancer Center, Stony Brook University, Stony Brook, NY, USA

    Yusuf A. Hannun

  7. Department of Medical Biophysics, University of Toronto, 67 College Street, Toronto, ON, M5G 2M1, Canada

    Jeffrey A. Medin

Authors
  1. E. Ellen Jones
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  2. Shaalee Dworski
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  3. Mustafa Kamani
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  4. Daniel Canals
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  5. Masayuki Wada
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  6. Xiang Liu
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  7. Yusuf A. Hannun
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  8. James Norris
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  9. Jeffrey A. Medin
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  10. Richard R. Drake
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Corresponding author

Correspondence to Richard R. Drake .

Editor information

Editors and Affiliations

  1. Stony Brook University, Stony Brook Cancer Center, Stony Brook, New York, USA

    Yusuf A. Hannun

  2. Dept. of Physiology & Biophysics, Stony Brook University, Stony Brook, New York, USA

    Chiara Luberto

  3. Dept. of Medicine, Stony Brook University, Stony Brook, New York, USA

    Cungui Mao

  4. Dept. of Medicine, Stony Brook University, Stony Brook, New York, USA

    Lina Marie Obeid

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© 2015 Springer International Publishing Switzerland

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Jones, E.E. et al. (2015). Detection and Distribution of Sphingolipids in Tissue by FTICR MALDI-Imaging Mass Spectrometry. In: Hannun, Y., Luberto, C., Mao, C., Obeid, L. (eds) Bioactive Sphingolipids in Cancer Biology and Therapy. Springer, Cham. https://doi.org/10.1007/978-3-319-20750-6_15

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