Abstract
The carbon input for cysteine synthesis is provided by O-acetyl-L-serine (OAS). Upon sulfur limitation, OAS levels increase, leading to the hypothesis that OAS regulates the expression of genes responsive to sulfur deficiency. Recently this hypothesis received support from a global co-expression analysis under conditions of variable OAS levels and constant sulfur status. This study identified six OAS cluster genes (SDI-1, SDI-2, LSU1, APR3, ChaC and SHM7), but the function of these genes and the signaling cascades regulating their expression remain uncharacterized. In our study, we provide additional proofs for the OAS-responsiveness of these genes by performing in silico analysis of their promoter regions together with the analysis of promoter::GUS lines.
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Acknowledgements
We thank the Max Planck Society (MPG) for financial support. We are grateful to Eugenia Maximova for assistance with the microscopic analyses.
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Aarabi, F., Hubberten, HM., Heyneke, E., Watanabe, M., Hoefgen, R. (2015). OAS Cluster Genes: A Tightly Co-regulated Network. In: De Kok, L., Hawkesford, M., Rennenberg, H., Saito, K., Schnug, E. (eds) Molecular Physiology and Ecophysiology of Sulfur. Proceedings of the International Plant Sulfur Workshop. Springer, Cham. https://doi.org/10.1007/978-3-319-20137-5_13
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DOI: https://doi.org/10.1007/978-3-319-20137-5_13
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