Abstract
Apoplastic iron (Ai) represents all iron (Fe) fractions located within the space delimited by the plasma membranes and the plant surface; Fe precipitations attached to the root surface (Di) are not included. Total extractable Fe (TEi) includes Ai and Di. Seven-day-old maize plants were grown hydroponically for 19 days under sulfate deprivation against a control. TEi and Ai concentrations were determined and their kinetics studied in each root type of maize plants grown under sulfate deprivation in samples taken at various hours (0.5, 1, 2, 3, 6) and days (7, 8, 9, 10, 17, 26) during each treatment. In order to remove any iron precipitations from the root surface, a treatment with dithionite-citrate-bicarbonate (DCB) was performed. After 10 days under sulfate deprivation, the total root iron (ROOTi) was lower than the control by 28 %, allocated as internal iron (INTi) by 96 %, Ai by 2 % and Di by 2 %. The Ai within the root system was allocated by 58 % in the embryonic roots (unchanged), 16 % in the mesocotyl roots (increased) and 26 % in the crown roots (decreased). After 19 days of sulfate deprivation, the ROOTi was lower than the control by 97 %, allocated as INTi by 57 %, Ai by 20.6 % and Di by 22.4 %. The Ai within the root system was allocated by 42 % in the embryonic roots (decreased), 34 % in the mesocotyl roots (increased) and 24 % in the crown roots (decreased). Crown roots suffered the largest decrease compared to the embryonic system, whilst the mesocotyl roots were richer in Ai.
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Maniou, F., Chorianopoulou, S.N., Bouranis, D.L. (2015). Apoplastic Iron Concentration in Maize Roots Grown Under Sulfate Deprivation. In: De Kok, L., Hawkesford, M., Rennenberg, H., Saito, K., Schnug, E. (eds) Molecular Physiology and Ecophysiology of Sulfur. Proceedings of the International Plant Sulfur Workshop. Springer, Cham. https://doi.org/10.1007/978-3-319-20137-5_11
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DOI: https://doi.org/10.1007/978-3-319-20137-5_11
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