Skip to main content
SpringerLink
Log in
Book cover

Plant Microtechniques and Protocols pp 153–165Cite as

Fluorescent Staining of Living Plant Cells

  • Chapter
  • First Online:

Abstract

Fluorescent dyes provide a convenient and sensitive method for assessing cell viability and visualizing organellar distribution and dynamics. This chapter describes various fluorescent dyes that are widely used to stain and image living plant cells and tissues.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   299.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   379.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  1. Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668

    Article  CAS  PubMed  Google Scholar 

  2. Fernández S, Osorio S, Heredia A (1999) Monitoring and visualizing plant cuticles by confocal laser scanning microscopy. Plant Physiol 37:789–794

    Google Scholar 

  3. Singh J, Devi S (1992) Development of fern sporangia: a fluorescence microscopy study. Biotech Histochem 67:261–264

    Article  CAS  PubMed  Google Scholar 

  4. Donaldson LA, Radotic K (2013) Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. J Microsc 251:178–187

    Article  CAS  PubMed  Google Scholar 

  5. Biggs AR (1985) Detection of impervious tissue in tree bark with selective histochemistry and fluorescence microscopy. Stain Technol 60:299–304

    CAS  PubMed  Google Scholar 

  6. Poustka F, Irani NG, Feller A, Lu Y et al (2007) A trafficking pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein-sorting route in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol 145:1323–1335

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Palevitz BA, O’Kane DJ, Kobres RE, Raikhel NV (1981) Vacuole movements and changes in morphology in differentiating cells as revealed by epifluorescence, video and electron microscopy. Protoplasma 109:23–55

    Article  Google Scholar 

  8. Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T (2008) Quantum dots versus organic dyes as fluorescent labels. Nat Methods 5:763–775

    Article  CAS  PubMed  Google Scholar 

  9. Tepfer M, Taylor IE (1981) The permeability of plant cell walls as measured by gel filtration chromatography. Science 213:761–763

    Article  CAS  PubMed  Google Scholar 

  10. Baron-Epel O, Charyal PK, Schindler M (1988) Pectins as mediators of wall porosity in soybean cells. Planta 175:389–395

    Article  CAS  PubMed  Google Scholar 

  11. Brière C, Barthou H, Petitprez M (2004) A new tool for plant cell biology: In vivo antibody uptake in plant protoplasts. Plant Cell Rep 12:878–884

    Google Scholar 

  12. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802–805

    Article  CAS  PubMed  Google Scholar 

  13. Tsien RY (1998) The green fluorescent protein. Ann Rev Biochem 67:509–544

    Article  CAS  PubMed  Google Scholar 

  14. Brandizzi F, Fricker M, Hawes C (2002) A greener world: the revolution in plant bioimaging. Nature 3:520–530

    CAS  Google Scholar 

  15. Held MA, Boulaflous A, Brandizzi F (2008) Advances in fluorescent protein-based imaging for the analysis of plant endomembranes. Plant Physiol 147:1469–1481

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  16. Herth W, Schnepf E (1980) The fluorochrome, calcofluor white, binds oriented to structural polysaccharide fibrils. Protoplasma 105:129–133

    Article  Google Scholar 

  17. Matzke MA, Matzke AJM (1986) Visualization of mitochondria and nuclei in living plant cells by the use of a potential-sensitive fluorescent dye. Plant Cell Environ 9:73–77

    Google Scholar 

  18. Terasaki M, Song J, Wong JR, Weiss MJ, Che LB (1984) Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 38:101–108

    Article  CAS  PubMed  Google Scholar 

  19. Johnson LV, Walsh ML, Chen LB (1980) Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci USA 77:990–994

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  20. Wu FS (1987) Localization of mitochondria in plant cells by vital staining with rhodamine 123. Planta 171:346–357

    Article  CAS  PubMed  Google Scholar 

  21. Traganos F, Darzynkiewicz Z, Sharpless T, Melamed MR (1977) Simultaneous staining of ribonucleic acids in unfixed cells using acridine orange in flow cytofluorometric system. J Histochem Cytochem 25:45–56

    Google Scholar 

  22. Kapusicinski J (1995) DAPI: a DNA-specific fluorescent probe. Biotech Histochem 70:220–233

    Article  Google Scholar 

  23. Barcellona ML, Cardiel G, Gratton E (1990) Time-resolved fluorescence of DAPI in solution and bound to polydeoxynucleotides. Biochem Biophys Res Commun 170:270–280

    Article  CAS  PubMed  Google Scholar 

  24. Timmers ACJ, Tirlapur UK, Schel JHN (1995) Vacuolar accumulation of acridine orange and neutral red in zygotic and somatic embryos of carrot (Daucus carota L.). Protoplasma 188:236–244

    Article  Google Scholar 

  25. Moore A, Donahue CJ, Bauer KD, Mather JP (1998) Simultaneous measurement of cell cycle and apoptotic cell death. Methods Cell Biol 57:265–278

    Article  CAS  PubMed  Google Scholar 

  26. Verbelen JP, Tao W (1998) Mobile arrays of vacuole ripples are common in plant cells. Plant Cell Rep 17:917–920

    Article  CAS  Google Scholar 

  27. El-Maarouf-Bouteau H, Moreau E, Errakhi R, Sallé G (2008) A diffusible signal from germinating Orobanche ramose elicits early defense responses in suspension-cultured Arabidopsis thaliana. Plant Signal Behav 3:189–193

    Article  PubMed Central  PubMed  Google Scholar 

  28. Lee EK, Jin YW, Park JH, Yoo YM et al (2010) Cultured cambial meristematic cells as a source of plant natural products. Nature Biotech 28:1213–1217

    Article  CAS  Google Scholar 

  29. Chen S, Halkier BA (2000) Characterization of glucosinolate uptake by leaf protoplasts of Brassica napus. J Biol Chem 275:22955–22960

    Article  CAS  PubMed  Google Scholar 

  30. Dubrovsky JG, Guttenberger M, Saralegui A, Napsucialy-Mendivil S, Voigt B, Baluska F, Menzel D (2006) Neutral red as a probe for confocal laser scanning microscopy studies of plant roots. Ann Bot 97:1127–1138

    Article  PubMed Central  PubMed  Google Scholar 

  31. Gonzalez ME, Jernstedt JA, Slaughter DC, Barrett DM (2010) Microscopic quantification of cell integrity in raw and processed onion parenchyma cells. J Food Sci 75:E402–E408

    Article  CAS  PubMed  Google Scholar 

  32. Inselman AL, Gathman AC, Lilly WW (1999) Two fluorescent markers identify the vacuolar system of Schizophyllum commune. Curr Microbiol 38:295–299

    Article  CAS  Google Scholar 

  33. Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining the viability of cultured cells. Stain Technol 47:189–194

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by Discovery Grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) and the University of Waterloo Start-Up Fund to SDXC.

Author information

Authors and Affiliations

  1. Department of Biology, University of Waterloo, 200 University Avenue West, N2L 3G1, Waterloo, Ontario, Canada

    Sarah Schoor, Shiu-Cheung Lung, Dustin Sigurdson & Simon D. X. Chuong

  2. School of Biological Sciences, University of Hong Kong, Hong Kong SAR, China

    Shiu-Cheung Lung

Authors
  1. Sarah Schoor
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shiu-Cheung Lung
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dustin Sigurdson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Simon D. X. Chuong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Simon D. X. Chuong .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary, CALGARY, Alberta, Canada

    Edward Chee Tak Yeung

  2. Department of Plant Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Claudio Stasolla

  3. Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Michael John Sumner

  4. Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, Minnesota, USA

    Bing Quan Huang

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Schoor, S., Lung, SC., Sigurdson, D., Chuong, S. (2015). Fluorescent Staining of Living Plant Cells. In: Yeung, E., Stasolla, C., Sumner, M., Huang, B. (eds) Plant Microtechniques and Protocols. Springer, Cham. https://doi.org/10.1007/978-3-319-19944-3_9

Download citation

Publish with us

Policies and ethics

Search

Navigation