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Whole-Mount Immunofluorescence Staining of Plant Cells and Tissues

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Plant Microtechniques and Protocols

Abstract

This chapter describes a method for preparing plant cells and tissues for whole-mount immunofluorescence microscopy. The protocol consists basically of four steps: fixation, partial enzymatic removal of cell walls, permeabilization of plasma membrane, and immunofluorescent staining. This protocol significantly shortens the time needed for specimen preparation as it does not require embedding and sectioning of cells and tissues. When stained samples are studied using a confocal laser scanning microscope (CLSM), three-dimensional distribution of the targeted organelle and antigen can be discerned. This technique augments the conventional immuno- and fluorescent staining procedures.

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Authors and Affiliations

  1. Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima, Japan

    Masaki Shimamura

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  1. Masaki Shimamura
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Correspondence to Masaki Shimamura .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary, CALGARY, Alberta, Canada

    Edward Chee Tak Yeung

  2. Department of Plant Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Claudio Stasolla

  3. Department of Biological Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Michael John Sumner

  4. Center for Basic Research in Digestive Disease, Mayo Clinic , Rochester, Minnesota, USA

    Bing Quan Huang

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Shimamura, M. (2015). Whole-Mount Immunofluorescence Staining of Plant Cells and Tissues. In: Yeung, E., Stasolla, C., Sumner, M., Huang, B. (eds) Plant Microtechniques and Protocols. Springer, Cham. https://doi.org/10.1007/978-3-319-19944-3_11

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