Abstract
In plant cell cultures aiming at the production of secondary metabolites of industrial interest, the culture medium composition is a decisive step for obtaining cell growth and high yields of the target compound(s). A rapid and reliable methodology for screening metabolic responses to medium composition is fundamental for the development of this biotechnological field. Following this approach, UV-Vis scanning spectrophotometry of callus extracts and their spectra pre-processing, univariate and multivariate analysis were tested in the present work. The results obtained successfully discriminated the culture media investigated and shed light on what metabolic pathways might be responsible for the differences among the callus cultures’ metabolic profiles.
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Acknowledgements:
To CAPES (Coordination for the Improvement of Higher Education Personnel) for financial support, Post-Graduation Program in Cell and Developmental Biology (Federal University of Santa Catarina) and BIOSYSTEMS research group (University of Minho).
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Pilatti, F.K., Costa, C., Rocha, M., Maraschin, M., Viana, A.M. (2015). UV-Visible Spectrophotometry-Based Metabolomic Analysis of Cedrela Fissilis Velozzo (Meliaceae) Calluses - A Screening Tool for Culture Medium Composition and Cell Metabolic Profiles. In: Overbeek, R., Rocha, M., Fdez-Riverola, F., De Paz, J. (eds) 9th International Conference on Practical Applications of Computational Biology and Bioinformatics. Advances in Intelligent Systems and Computing, vol 375. Springer, Cham. https://doi.org/10.1007/978-3-319-19776-0_4
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