Abstract
The carotid body is a multi-modal sensor and it has been debated if it senses low glucose. We have hypothesized that the carotid body is modified by some metabolic factors other than glucose and contributes to whole body glucose metabolism. This study examined the roles of insulin, leptin and transient receptor potential (TRP) channels on carotid sinus nerve (CSN) chemoreceptor discharge. In agreement with other studies, CSN activity was not modified by low glucose. Insulin did not affect the CSN hypoxic response. Leptin significantly augmented the CSN response to hypoxia and nonspecific Trp channel blockers (SKF96365, 2-APB) reversed the effect of leptin. Gene expression analysis showed high expression of Trpm3, 6, and 7 channels in the carotid body and petrosal ganglion. The results suggest that the adult mouse carotid body does not sense glucose levels directly. The carotid body may contribute to neural control of glucose metabolism via leptin receptor-mediated TRP channel activation.
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References
Alvarez-Buylla R, Alvarez-Buylla ER (1988) Carotid sinus receptors participate in glucose homeostasis. Respir Physiol 72:347–360
Balbir A, Lee H, Okumura M, Biswal S, Fitzgerald RS, Shirahata M (2007) A search for genes that may confer divergent morphology and function in the carotid body between two strains of mice. Am J Physiol Lung Cell Mol Physiol 292:L704–L715
Bin-Jaliah I, Maskell PD, Kumar P (2004) Indirect sensing of insulin-induced hypoglycaemia by the carotid body in the rat. J Physiol 556:255–266
Conde SV, Obeso A, Gonzalez C (2007) Low glucose effects on rat carotid body chemoreceptor cells’ secretory responses and action potential frequency in the carotid sinus nerve. J Physiol 585:721–730
Gallego-Martin T, Fernandez-Martinez S, Rigual R, Obeso A, Gonzalez C (2012) Effects of low glucose on carotid body chemoreceptor cell activity studied in cultures of intact organs and in dissociated cells. Am J Physiol Cell Physiol 302:C1128–C1140
Gonzalez C, Almaraz L, Obeso A, Rigual R (1994) Carotid body chemoreceptors: from natural stimuli to sensory discharges. Physiol Rev 74:829–898
Koyama Y, Coker RH, Stone EE, Lacy DB, Jabbour K, Williams PE, Wasserman DH (2000) Evidence that carotid bodies play an important role in glucoregulation in vivo. Diabetes 49:1434–1442
Pardal R, López-Barneo J (2002) Low glucose-sensing cells in the carotid body. Nat Neurosci 5:197–198
Porzionato A, Rucinski A, Macchi V, Stecco C, Castagliuoloc I, Malendowicz LK, De Caro R (2011) Expression of leptin and leptin receptor isoforms in the rat and human carotid body. Brain Res 1385:56–67
Qiu J, Fang Y, Rønnekleiv OK, Kelly MJ (2010) Leptin excites POMC neurons via activation of TRPC channels. J Neurosci 30:1560–1565
Shin MK, Yao Q, Jun JC, Bevans-Fonti S, Yoo DY, Han W, Mesarwi O, Richardson R, Fu YY, Pasricha PJ, Schwartz AR, Shirahata M, Polotsky VY (2014) Carotid body denervation prevents fasting hyperglycemia during chronic intermittent hypoxia. J Appl Physiol 117:765–776
Tankersley CG, Tang W-Y, Wang X, Pardo C, Bierman A, Shirahata M (2013) Effect of leptin on hypoxic ventilator regulation. ATS international conference A5763
Wehrwein EA, Basu R, Basu A, Curry TB, Rizza RA, Joyner MJ (2010) Hyperoxia blunts counterregulation during hypoglycaemia in humans: possible role for the carotid bodies? J Physiol 588:4593–4601
Zhang M, Buttigieg J, Nurse CA (2007) Neurotransmitter mechanisms mediating low-glucose signalling in cocultures and fresh tissue slices of rat carotid body. J Physiol 578:735–750
Acknowledgements
This work was funded, in part, by HL081345, ES016817, HL080105, and AHA 12POST118200001.
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Shirahata, M., Tang, WY., Shin, MK., Polotsky, V.Y. (2015). Is the Carotid Body a Metabolic Monitor?. In: Peers, C., Kumar, P., Wyatt, C., Gauda, E., Nurse, C., Prabhakar, N. (eds) Arterial Chemoreceptors in Physiology and Pathophysiology. Advances in Experimental Medicine and Biology, vol 860. Springer, Cham. https://doi.org/10.1007/978-3-319-18440-1_17
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DOI: https://doi.org/10.1007/978-3-319-18440-1_17
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