Abstract
Optical recording with fast voltage sensitive dyes makes it possible, in suitable preparations, to simultaneously monitor the action potentials of large numbers of individual neurons. Here we describe methods for doing this, including considerations of different dyes and imaging systems, methods for correlating the optical signals with their source neurons, procedures for getting good signals, and the use of Independent Component Analysis for spike-sorting raw optical data into single neuron traces. These combined tools represent a powerful approach for large-scale recording of neural networks with high temporal and spatial resolution.
Keywords
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Acknowledgments
Supported by NIH NS060921, NSF 1257923, Dart Foundation and Grass Foundation Marine Biological Laboratory summer fellowships, the Fred B. Snite Foundation, Rosalind Franklin University of Medicine and Science (WF), NIH F31NS079036 (AB), MRC Senior non-Clinical Fellowship (MH) and the Howard Hughes Medical Institute (TS).
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Frost, W.N. et al. (2015). Monitoring Spiking Activity of Many Individual Neurons in Invertebrate Ganglia. In: Canepari, M., Zecevic, D., Bernus, O. (eds) Membrane Potential Imaging in the Nervous System and Heart. Advances in Experimental Medicine and Biology, vol 859. Springer, Cham. https://doi.org/10.1007/978-3-319-17641-3_5
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DOI: https://doi.org/10.1007/978-3-319-17641-3_5
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