Abstract
A short summary of the main subjects described in this book is presented. The main differences between in vitro and in vivo conditions and their implications for the methodology adopted in both situations are discussed. The mitochondrial metabolic states described by Chance and Williams in 1955 are compared to in vivo conditions. In addition, the use of NADH fluorescence together with other physiological parameters in patients is discussed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Harrois A, Dupic L, Duranteau J (2011) Targeting the microcirculation in resuscitation of acutely unwell patients. Curr Opin Crit Care 17(3):303–307. doi:10.1097/MCC.0b013e3283466ba0
Chance B, Nioka S, Warren W, Yurtsever G (2005) Mitochondrial NADH as the bellwether of tissue O2 delivery. Adv Exp Med Biol 566:231–242
Singer M (2007) Mitochondrial function in sepsis: acute phase versus multiple organ failure. Crit Care Med 35(9 suppl):S441–S448. doi:10.1097/01.CCM.0000278049.48333.78
Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. In: Nord FF (ed) Advances in enzymology and related areas of molecular biology, XVIIth edn. Interscience, New York, pp 65–134
Chance B, Williams GR (1954) Steady-state of reduced pyridine nucleotides in phosphorylating rat liver mitochondria. Am Soc Biol Chem 13 (Abstract 633)
Connelly CM, Chance B (1954) Kinetics of reduced pyridine nucleotides in stimulated frog muscle and nerve. Am Physiol Soc 13(1):29
Chance B, Williams GR (1955) Respiratory enzymes in oxidative phosphorylation. III. The steady state. J Biol Chem 217:409–427
Chance B, Williams GR (1955) A method for the localization of sites for oxidative phosphorylation. Nature (Lond) 176(4475):250–254
Chance B, Jobsis F (1959) Changes in fluorescence in a frog sartorius muscle following a twitch. Nature (Lond) 184:195–196
Chance B, Legallias V, Schoener B (1962) Metabolically linked changes in fluorescence emission spectra of cortex of rat brain, kidney and adrenal gland. Nature (Lond) 195:1073–1075
Chance B, Schoener B (1962) Correlation of oxidation-reduction changes of intracellular reduced pyridine nucleotide and changes in electro-encephalogram of the rat in anoxia. Nature (Lond) 195:956–958
Chance B, Schoener B (1962) Control of oxidation-reduction state of NADH in the liver of anesthetized rats. Symp Regul Enzyme Act Synth Norm Neoplast Tissues Proc 1:169–185
Chance B, Schoener B, Fergusson JJ (1962) In vivo induced oxidation by adrenocorticotrophic hormone of reduced pyridine nucleotide in the adrenal cortex of hypophysectomized rats. Nature (Lond) 195:776–778
Chance B, Cohen P, Jobsis F, Schoener B (1962) Intracellular oxidation-reduction states in vivo. Science 137:499–508
Chance B, Cohen P, Jobsis F, Schoener B (1962) Localized fluorometry of oxidation-reduction states of intracellular pyridine nucleotide in brain and kidney cortex of the anesthetized rat. Science 136:325. doi:10.1126/science.136.3513.325
Vatov L, Kizner Z, Ruppin E, Meilin S, Manor T, Mayevsky A (2006) Modeling brain energy metabolism and function: a multiparametric monitoring approach. Bull Math Biol 68:275–291
Hanson CW 3rd, Marshall BE (2001) Artificial intelligence applications in the intensive care unit. Crit Care Med 29(2):427–435
Bates JH, Young MP (2003) Applying fuzzy logic to medical decision making in the intensive care unit. Am J Respir Crit Care Med 167(7):948–952. doi:10.1164/rccm.200207-777CP
Kutai-Asis H, Barbiro-Michaely E, Deutsch A, Mayevsky A (2006) Fiber optic based multiparametric spectroscopy in vivo: toward a new quantitative tissue vitality index. SPIE Proc 6083:10-10–10-11
Waltemath CL (1970) Oxygen, uptake, transport, and tissue utilization. Anesth Analg 49(1):184–203
Mayevsky A, Frank K, Muck M, Nioka S, Kessler M, Chance B (1992) Multiparametric evaluation of brain functions in the Mongolian gerbil in vivo. J Basic Clin Physiol Pharmacol 3:323–342
Mayevsky A, Lebourdais S, Chance B (1980) The interrelation between brain PO2 and NADH oxidation-reduction state in the gerbil. J Neurosci Res 5:173–182
Mayevsky A, Meilin S, Manor T, Ornstein E, Zarchin N, Sonn J (1998) Multiparametric monitoring of brain oxygen balance under experimental and clinical conditions. Neurol Res 20:S76–S80
Mandelbaum MM, Barbiro-Michaely E, Tolmasov M, Mayevsky A (2008) Effects of severe hemorrhage on in vivo brain and small intestine mitochondrial NADH and microcirculatory blood flow. J Innov Opt Health Sci 1:177–183. doi:10.1142/S1793545808000261
Mayevsky A, Walden R, Pewzner E, Deutsch A, Heldenberg E, Lavee J, Tager S, Kachel E, Raanani E, Preisman S, Glauber V, Segal E (2011) Mitochondrial function and tissue vitality: bench-to-bedside real-time optical monitoring system. J Biomed Optics 16(067004):067001–067021. doi:10.1117/1.3585674
Mayevsky A, Rogatsky G (2007) Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies. Am J Physiol Cell Physiol 292:C615–C640
Mayevsky A, Meilin S, Manor T, Zarchin N, Sonn J (1999) Optical monitoring of NADH redox state and blood flow as indicators of brain energy balance. Adv Exp Med Biol 471:133–140
Mayevsky A (1993) Biochemical and physiological activities of the brain as in vivo markers of brain pathology. In: Bernstein EF, Callow AD, Nicolaides AN, Shifrin EG (eds) Cerebral revascularization. Med-Orion, Springfield Lakes, pp 51–69
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Mayevsky, A. (2015). Discussion and Conclusions. In: Mitochondrial Function In Vivo Evaluated by NADH Fluorescence. Springer, Cham. https://doi.org/10.1007/978-3-319-16682-7_10
Download citation
DOI: https://doi.org/10.1007/978-3-319-16682-7_10
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-16681-0
Online ISBN: 978-3-319-16682-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)