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A Novel Cysteine Sulfinic Acid Decarboxylase Knock-Out Mouse: Immune Function

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Taurine 9

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 803))

Abstract

We have studied the anti-inflammatory effects of taurine using both cat and rodent models with or without taurine supplementation in the food or drinking water, respectively. Our previous data demonstrate that taurine deficient cats have altered immune functions. Both cats and rodents have limitations for understanding the immune function of taurine. We developed a novel CSAD KO mouse to investigate immune function. The CSAD KO mouse has low plasma taurine concentrations (86 %) and low taurine concentration in the liver (95 %) compared to WT. However, taurine concentrations in the G3 CSAD−/− splenocytes (56 %) were decreased to a lesser extent than was plasma and liver taurine concentration. There was no difference in spleen size between wild type (WT) and homozygote mice (G3 CSAD−/−). Lipopolysaccharide (LPS), a mitogen for B cells, and Concanavalin A (Con A), a mitogen for T cells, was used for lymphocyte proliferation in spleen cells measured using 3H-thymidine incorporation. LPS-induced cellular proliferation was significantly decreased in spleen cells from both G3 CSAD−/− (p < 0.01) females (33 %) and males (51 %). In contrast, Con A induced cellular proliferation was significantly decreased in only females from G3 CSAD−/− (p < 0.01) (65 %). In order to examine whether exogenous taurine may affect lymphocyte transformation, 10 or 50 mM taurine was added in vitro to LPS and Con A stimulated spleen cells from G3 CSAD−/−. Both 10 and 50 mM taurine in WT and CSAD−/− were without effect.

These data implicate that taurine may be actively transported into the spleen through taurine transporter because of higher taurine concentrations in the G3 CSAD−/− splenocytes (44 % of WT) compared to those in the G3 CSAD−/− liver (10 % of WT) and plasma (20 % of WT). CSAD KO may have immune defects in both B cells important for antibody production and T cells involved in acquired cellular immunity. These changes in immune function may be absence of CSAD gene but not due to lower taurine levels in CSAD KO.

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Abbreviations

CSAD:

Cysteine sulfinic acid decarboxylase

CSAD KO:

Cysteine sulfinic acid decarboxylase knockout mice

G1:

G2 and G3, Generation 1, 2 and 3

G1 HO:

HO (CSAD−/−) mice born from HT (CSAD+/−) parents

G2 or G3 HO:

Mice born from G1 HO or G2 HO parents

HO:

Homozygotic mice (CSAD−/−)

HT:

Heterozygotic mice (CSAD+/−)

Tau T:

Taurine transporter

WT:

Wild type (CSAD+/+)

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Acknowledgements

This work was supported by the Office for People with Developmental Disabilities, Albany, NY and Dong A Pharmaceutical Co., LTD, Seoul, Korea. We are thankful to Dr. William Levis for discussing the research and reviewing this manuscript.

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Authors and Affiliations

  1. School of Veterinary Medicine, Konkuk University, Seoul, Korea

    Seung Yong Park

  2. Department of Developmental Neurobiology, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY, 10314, USA

    Georgia Schuller-Levis & Eunkyue Park

Authors
  1. Seung Yong Park
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  2. Georgia Schuller-Levis
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  3. Eunkyue Park
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Correspondence to Eunkyue Park .

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Editors and Affiliations

  1. Chair of Immunology, Medical College Jagiellonian University, Krakow, Poland

    Janusz Marcinkiewicz

  2. Department of Pharmacology, College of Medicine University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

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Park, S.Y., Schuller-Levis, G., Park, E. (2015). A Novel Cysteine Sulfinic Acid Decarboxylase Knock-Out Mouse: Immune Function. In: Marcinkiewicz, J., Schaffer, S. (eds) Taurine 9. Advances in Experimental Medicine and Biology, vol 803. Springer, Cham. https://doi.org/10.1007/978-3-319-15126-7_8

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