Abstract
In acute inflammation, neutrophils are activated in response to several agonists generating reactive oxygen species (ROS) by NADPH oxidase-dependent mechanisms. Thiotaurine, a thiosulfonate structurally related to hypotaurine and taurine, is a metabolic product of cystine and contains sulfane sulfur which can play several regulatory roles in biological systems. In the present study, the effects of thiotaurine on human leukocyte activation have been evaluated. Neutrophils were stimulated by phorbol 12-myristate 13-acetate (PMA) or chemotactic peptide (fMLP) and respiratory burst was assessed by luminol enhanced chemiluminescence. Superoxide generation was estimated by measuring cytochrome c reduction. Thiotaurine caused a decrease of the respiratory burst of PMA-stimulated neutrophils in a dose-dependent manner, while fMLP-stimulated cells were slightly affected by thiotaurine. Addition of 100 μM thiotaurine induced 64.4 % inhibition of ROS production by PMA-activated cells, as evaluated by luminol enhanced chemiluminescence. As thiotaurine can release sulfane sulfur producing hydrogen sulfide (H2S) and hypotaurine, the effects of these compounds on leukocyte functional responses were evaluated and compared with those exerted by thiotaurine, which had proved more effective in inhibiting respiratory burst than H2S, hypotaurine and taurine. However, fMLP-induced azurophilic degranulation as analyzed by elastase release was unaffected by thiotaurine up to 5 mM concentration. Compared with fMLP-activated neutrophils, PMA-activated neutrophils were more susceptible to thiotaurine inhibition, suggesting that thiotaurine may interfere with the PKC-dependent pathway of neutrophil activation.
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Abbreviations
- fMLP:
-
N-formyl-methionyl-leucyl-phenylalanine
- HTAU:
-
Hypotaurine
- PMA:
-
Phorbol 12-myristate 13-acetate
- PKC:
-
Protein kinase C
- ROS:
-
Reactive oxygen species
- TAU:
-
Taurine
- TTAU:
-
Thiotaurine
References
Acharya M, Lau-Cam CA (2013) Comparative evaluation of the effects of taurine and thiotaurine on alterations of the cellular redox status and activities of antioxidant and glutathione-related enzymes by acetaminophen in the rat. Adv Exp Med Biol 776:199–215
Aruoma OI, Halliwell B, Hoey BM, Butler J (1998) The antioxidant action of taurine, hypotaurine and their metabolic precursor. Biochem J 256:251–255
Beinert H (2000) A tribute to sulfur. Eur J Biochem 267:5657–5664
Budhram R, Pandya KG, Lau-Cam CA (2013) Protection by taurine and thiotaurine against biochemical and cellular alterations induced by diabetes in a rat model. Adv Exp Med Biol 775:321–343
Capuozzo E, Pecci L, Baseggio Conrado A, Fontana M (2013) Thiotaurine prevents apoptosis of human neutrophils: a putative role in inflammation. Adv Exp Med Biol 775:227–236
Cavallini D, De Marco C, Mondovì B (1959) Chromatographic evidence of the occurrence of thiotaurine in the urine of rats fed with cystine. J Biol Chem 234:854–857
Cavallini D, De Marco C, Mondovì B, Tentori L (1960) Radioautographic detection of metabolites of 35S-DL-cystine. J Chromatog 3:20–24
Chauncey TR, Westley J (1983) The catalytic mechanism of yeast thiosulfate reductase. J Biol Chem 258:15037–15045
Choi HS, Cha YN, Kim C (2006) Taurine chloramine inhibits PMA-stimulated superoxide production in human neutrophils perhaps by inhibiting phosphorylation and translocation of p47phox. Int Immunopharmacol 6:1431–1440
De Marco C, Tentori L (1961) Sulfur exchange between thiotaurine and hypotaurine. Experientia 17:345–346
De Marco C, Coletta M, Cavallini D (1961) Spontaneous transulfuration of sulfinates by organic persulfides. Arch Biochem Biophys 93:179–180
Dewas C, Fay M, Gougerot-Pocidalo MA, El-Benna J (2000) The mitogen-activated protein kinase extracellular signal-regulated kinase 1/2 pathway is involved in formyl-methionyl-leucyl-phenylalanine-induced p47phox phosphorylation in human neutrophils. J Immunol 165:5238–5244
El Benna J, Han J, Park JW, Schimd E, Ulevitch RJ, Babior BM (1996) Activation of p38 in stimulated human neutrophils: phosphorylation of the oxidase component p47phox by p38 and ERK but not by JNK. Arch Biochem Biophys 334:395–400
Ferrante A, Thong YH (1980) Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leukocytes from human peripheral blood by the Hypaque-Ficoll method. J Immunol Methods 36:109–117
Fontana M, Pecci L, Duprè S, Cavallini D (2004) Antioxidant properties of sulfinates: protective effect of hypotaurine on peroxynitrite-dependent damage. Neurochem Res 29:111–116
Green TR, Fellman JH, Eichert AL, Pratt KL (1991) Antioxidant role and subcellular localisation of hypotaurine and taurine in human neutrophils. Biochim Biophys Acta 1073:91–97
Huxtable RJ (1992) Physiological actions of Taurine. Physiol Rev 72:101–163
Kim C, Cha YN (2014) Taurine chloramine produced from taurine under inflammation provides anti-inflammatory and cytoprotective effects. Amino Acids 46:89–100
Klink M, Tchorzewski H, Sulowska Z (2003) Oxidative and adhesive responses of human neutrophils to nitrovasodilators in vitro. The role of protein kinases. Mediators Inflamm 12:345–353
Learn DB, Fried VA, Thomas EL (1990) Taurine and hypotaurine content of human leukocytes. J Leukoc Biol 48:174–182
Lehmeyer JE, Snyderman R, Johnston RB (1979) Stimulation of neutrophil oxidative metabolism by chemotactic peptides: influence of calcium ion concentration and cytochalasin B and comparison with stimulation by phorbol myristate acetate. Blood 54:35–45
Marcinkiewicz J, Kontny E (2014) Taurine and inflammatory diseases. Amino Acids 46:7–20
Morel F, Doussiere J, Vignais PV (1991) The superoxide-generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects. Eur J Biochem 201:523–546
Mueller EG (2006) Trafficking in persulfides: delivering sulfur in biosynthetic pathways. Nat Chem Biol 2:185–194
Nick JA, Avdi NJ, Young SK, Knall C, Gerwins P, Johnson GL, Worthen GS (1997) Common and distinct intracellular signaling pathways in human neutrophils utilized by platelet activating factor and FMLP. J Clin Invest 99:975–986
Predmore BL, Lefer DJ, Gojon G (2012) Hydrogen sulfide in biochemistry and medicine. Antioxid Redox Signal 17:119–140
Schuller-Levis GB, Park E (2004) Taurine and its chloramines: modulators of immunity. Neurochem Res 29:117–126
Sklar LA, McNeil VM, Jesaitis AJ, Painter RG, Cochrane CG (1982) A continuous, spectroscopic analysis of the kinetics of elastase secretion by neutrophils. The dependence of secretion upon receptor occupancy. J Biol Chem 257:5471–5475
Sörbo B (1957) Enzymic transfer of sulfur from mercaptopyruvate to sulfite or sulfinates. Biochim Biophys Acta 24:324–329
Toohey JI (1989) Sulphane sulphur in biological systems: a possible regulatory role. Biochem J 264:625–632
Toohey JI (2011) Sulfur signalling: is the agent sulfide or sulfane? Anal Biochem 413:1–7
Westley J, Heyse D (1971) Mechanisms of sulfur transfer catalysis. Sulfhydryl-catalyzed transfer of thiosulfonate sulfur. J Biol Chem 246:1468–1474
Zanardo RCO, Brancaleone V, Distrutti E, Fiorucci S, Cirino G, Wallace JL (2006) Hydrogen sulfide is an endogenous modulator of leukocyte-mediated inflammation. FASEB J 20:2118–2120
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Capuozzo, E., Conrado, A.B., Fontana, M. (2015). Thiotaurine Modulates Human Neutrophil Activation. In: Marcinkiewicz, J., Schaffer, S. (eds) Taurine 9. Advances in Experimental Medicine and Biology, vol 803. Springer, Cham. https://doi.org/10.1007/978-3-319-15126-7_13
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DOI: https://doi.org/10.1007/978-3-319-15126-7_13
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