Abstract
In plants, as in humans, splicing regulation controls plant growth and development. Plant CK2 is involved in multiple signaling pathways. The maize CK2β1 regulatory subunit is preferentially accumulated in nuclear speckles, and little is known about the role of CK2 in these nuclear structures that contain splicing regulators among other proteins. Here we perform a yeast two-hybrid screening using CK2β1 as a bait, and between other putative partners, we found an RNA-binding protein that presents high homology with Arabidopsis TGH, an evolutionary conserved protein required for proper plant development. In addition, transient co-transformation of both proteins in maize embryo cells confirms that maize CK2β1 regulatory subunit and ZmTGH colocalize in nuclear speckles. The interaction and colocalization of both proteins suggest a possible role of CK2 modulating proteins involved in splicing processes in plants.
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Vélez-Bermúdez, I.C., Riera, M. (2015). Maize RNA-Binding Protein ZmTGH: A New Partner for CK2β1 Regulatory Subunit. In: Ahmed, K., Issinger, OG., Szyszka, R. (eds) Protein Kinase CK2 Cellular Function in Normal and Disease States. Advances in Biochemistry in Health and Disease, vol 12. Springer, Cham. https://doi.org/10.1007/978-3-319-14544-0_4
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DOI: https://doi.org/10.1007/978-3-319-14544-0_4
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