Skip to main content
SpringerLink
Log in

Homotypic Cell–Cell Interactions and Apicobasal Polarity in Epithelial Cells and Endothelial Cells

  • Chapter
  • First Online:
Cell Polarity 1

  • 1358 Accesses

Abstract

Epithelial and endothelial cells are embedded in sheets of cells and have a pronounced apicobasal polarity. The development of apicobasal polarity is regulated by a conserved set of cell polarity proteins which regulate different aspects of cellular polarization in various contexts. In epithelial and endothelial cells, apicobasal polarity is initiated when cells start to form cell–cell contacts and long before the cells have developed mature cell–cell junctions and have acquired a fully polarized phenotype. Cell–cell adhesion has turned out to be intimately involved in the regulation of polarization. In this chapter, we will highlight the molecular mechanisms through which cell–cell adhesion molecules act in concert with cell polarity proteins to regulate various aspects of cellular polarization in vertebrate epithelial and endothelial cells.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Abe K, Takeichi M (2008) EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt. Proc Natl Acad Sci U S A 105(1):13–19

    PubMed Central  CAS  PubMed  Google Scholar 

  • Adams CL, Nelson WJ, Smith SJ (1996) Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion. J Cell Biol 135(6 Pt 2):1899–1911

    CAS  PubMed  Google Scholar 

  • Aigner K, Dampier B, Descovich L, Mikula M, Sultan A, Schreiber M, Mikulits W, Brabletz T, Strand D, Obrist P, Sommergruber W, Schweifer N, Wernitznig A, Beug H, Foisner R, Eger A (2007) The transcription factor ZEB1 (deltaEF1) promotes tumour cell dedifferentiation by repressing master regulators of epithelial polarity. Oncogene 26(49):6979–6988. doi:10.1038/sj.onc.1210508

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ando-Akatsuka Y, Yonemura S, Itoh M, Furuse M, Tsukita S (1999) Differential behavior of E-cadherin and occludin in their colocalization with ZO-1 during the establishment of epithelial cell polarity. J Cell Physiol 179(2):115–125

    CAS  PubMed  Google Scholar 

  • Aricescu AR, Jones EY (2007) Immunoglobulin superfamily cell adhesion molecules: zippers and signals. Curr Opin Cell Biol 19(5):543–550. doi:10.1016/j.ceb.2007.09.010

    CAS  PubMed  Google Scholar 

  • Asakura T, Nakanishi H, Sakisaka T, Takahashi K, Mandai K, Nishimura M, Sasaki T, Takai Y (1999) Similar and differential behaviour between the nectin-afadin-ponsin and cadherin-catenin systems during the formation and disruption of the polarized junctional alignment in epithelial cells. Genes Cells 4(10):573–581

    CAS  PubMed  Google Scholar 

  • Baas AF, Kuipers J, van der Wel NN, Batlle E, Koerten HK, Peters PJ, Clevers HC (2004) Complete polarization of single intestinal epithelial cells upon activation of LKB1 by STRAD. Cell 116(3):457–466

    CAS  PubMed  Google Scholar 

  • Barrios-Rodiles M, Brown KR, Ozdamar B, Bose R, Liu Z, Donovan RS, Shinjo F, Liu Y, Dembowy J, Taylor IW, Luga V, Przulj N, Robinson M, Suzuki H, Hayashizaki Y, Jurisica I, Wrana JL (2005) High-throughput mapping of a dynamic signaling network in mammalian cells. Science 307(5715):1621–1625

    CAS  PubMed  Google Scholar 

  • Bazzoni G, Dejana E (2004) Endothelial cell-to-cell junctions: molecular organization and role in vascular homeostasis. Physiol Rev 84(3):869–901. doi:10.1152/physrev.00035.2003

    CAS  PubMed  Google Scholar 

  • Benton R, St Johnston D (2003) Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. Cell 115(6):691–704

    CAS  PubMed  Google Scholar 

  • Brasch J, Harrison OJ, Honig B, Shapiro L (2012) Thinking outside the cell: how cadherins drive adhesion. Trends Cell Biol 22(6):299–310. doi:10.1016/j.tcb.2012.03.004

    PubMed Central  CAS  PubMed  Google Scholar 

  • Cano A, Perez-Moreno MA, Rodrigo I, Locascio A, Blanco MJ, del Barrio MG, Portillo F, Nieto MA (2000) The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat Cell Biol 2(2):76–83

    CAS  PubMed  Google Scholar 

  • Carmeliet P, Lampugnani MG, Moons L, Breviario F, Compernolle V, Bono F, Balconi G, Spagnuolo R, Oostuyse B, Dewerchin M, Zanetti A, Angellilo A, Mattot V, Nuyens D, Lutgens E, Clotman F, de Ruiter MC, Gittenberger-de Groot A, Poelmann R, Lupu F, Herbert JM, Collen D, Dejana E (1999) Targeted deficiency or cytosolic truncation of the VE-cadherin gene in mice impairs VEGF-mediated endothelial survival and angiogenesis. Cell 98(2):147–157

    CAS  PubMed  Google Scholar 

  • Comijn J, Berx G, Vermassen P, Verschueren K, van Grunsven L, Bruyneel E, Mareel M, Huylebroeck D, van Roy F (2001) The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol Cell 7(6):1267–1278

    CAS  PubMed  Google Scholar 

  • den Elzen N, Buttery CV, Maddugoda MP, Ren G, Yap AS (2009) Cadherin adhesion receptors orient the mitotic spindle during symmetric cell division in mammalian epithelia. Mol Biol Cell 20(16):3740–3750

    Google Scholar 

  • Du D, Pedersen E, Wang Z, Karlsson R, Chen Z, Wu X, Brakebusch C (2009) Cdc42 is crucial for the maturation of primordial cell junctions in keratinocytes independent of Rac1. Exp Cell Res 315(8):1480–1489

    CAS  PubMed  Google Scholar 

  • Du Q, Macara IG (2004) Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell 119(4):503–516

    CAS  PubMed  Google Scholar 

  • Du Q, Stukenberg PT, Macara IG (2001) A mammalian partner of inscuteable binds NuMA and regulates mitotic spindle organization. Nat Cell Biol 3(12):1069–1075. doi:10.1038/ncb1201-1069

    CAS  PubMed  Google Scholar 

  • Dujardin DL, Vallee RB (2002) Dynein at the cortex. Curr Opin Cell Biol 14(1):44–49

    CAS  PubMed  Google Scholar 

  • Durgan J, Kaji N, Jin D, Hall A (2011) Par6B and atypical PKC (aPKC) regulate mitotic spindle orientation during epithelial morphogenesis. J Biol Chem 286(14):12461–12471

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ebnet K, Aurrand-Lions M, Kuhn A, Kiefer F, Butz S, Zander K, Meyer Zu Brickwedde MK, Suzuki A, Imhof BA, Vestweber D (2003) The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity. J Cell Sci 116:3879–3891

    CAS  PubMed  Google Scholar 

  • Ebnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG, Vestweber D (2000) Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1. J Biol Chem 275(36):27979–27988

    CAS  PubMed  Google Scholar 

  • Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu Brickwedde MK, Ohno S, Vestweber D (2001) The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM). EMBO J 20(14):3738–3748

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ebnet K, Suzuki A, Ohno S, Vestweber D (2004) Junctional adhesion molecules (JAMs): more molecules with dual functions? J Cell Sci 117(1):19–29

    CAS  PubMed  Google Scholar 

  • Ehrlich JS, Hansen MD, Nelson WJ (2002) Spatio-temporal regulation of Rac1 localization and lamellipodia dynamics during epithelial cell-cell adhesion. Dev Cell 3(2):259–270

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ekblom P (1989) Developmentally regulated conversion of mesenchyme to epithelium. FASEB J 3(10):2141–2150

    CAS  PubMed  Google Scholar 

  • Elbediwy A, Zihni C, Terry SJ, Clark P, Matter K, Balda MS (2012) Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex. J Cell Biol 198(4):677–693

    PubMed Central  CAS  PubMed  Google Scholar 

  • Fleming ES, Zajac M, Moschenross DM, Montrose DC, Rosenberg DW, Cowan AE, Tirnauer JS (2007) Planar spindle orientation and asymmetric cytokinesis in the mouse small intestine. J Histochem Cytochem 55(11):1173–1180. doi:10.1369/jhc.7A7234.2007

    PubMed Central  CAS  PubMed  Google Scholar 

  • Fukuhara T, Shimizu K, Kawakatsu T, Fukuyama T, Minami Y, Honda T, Hoshino T, Yamada T, Ogita H, Okada M, Takai Y (2004) Activation of Cdc42 by trans interactions of the cell adhesion molecules nectins through c-Src and Cdc42-GEF FRG. J Cell Biol 166(3):393–405

    PubMed Central  CAS  PubMed  Google Scholar 

  • Fukuyama T, Ogita H, Kawakatsu T, Fukuhara T, Yamada T, Sato T, Shimizu K, Nakamura T, Matsuda M, Takai Y (2005) Involvement of the c-Src-Crk-C3G-Rap1 signaling in the nectin-induced activation of Cdc42 and formation of adherens junctions. J Biol Chem 280(1):815–825

    CAS  PubMed  Google Scholar 

  • Giannotta M, Trani M, Dejana E (2013) VE-cadherin and endothelial adherens junctions: active guardians of vascular integrity. Dev Cell 26(5):441–454. doi:10.1016/j.devcel.2013.08.020

    CAS  PubMed  Google Scholar 

  • Goldstein B (1995) Cell contacts orient some cell division axes in the Caenorhabditis elegans embryo. J Cell Biol 129(4):1071–1080

    CAS  PubMed  Google Scholar 

  • Green KJ, Simpson CL (2007) Desmosomes: new perspectives on a classic. J Invest Dermatol 127(11):2499–2515. doi:10.1038/sj.jid.5701015

    CAS  PubMed  Google Scholar 

  • Green RA, Wollman R, Kaplan KB (2005) APC and EB1 function together in mitosis to regulate spindle dynamics and chromosome alignment. Mol Biol Cell 16(10):4609–4622. doi:10.1091/mbc.E05-03-0259

    PubMed Central  CAS  PubMed  Google Scholar 

  • Gumbiner BM (1996) Cell adhesion: the molecular basis of tissue architecture and morphogenesis. Cell 84(3):345–357

    CAS  PubMed  Google Scholar 

  • Gumbiner BM (2005) Regulation of cadherin-mediated adhesion in morphogenesis. Nat Rev Mol Cell Biol 6(8):622–634

    CAS  PubMed  Google Scholar 

  • Hao Y, Boyd L, Seydoux G (2006) Stabilization of cell polarity by the C. elegans RING protein PAR-2. Dev Cell 10(2):199–208

    PubMed Central  CAS  PubMed  Google Scholar 

  • Harder JL, Whiteman EL, Pieczynski JN, Liu CJ, Margolis B (2012) Snail destabilizes cell surface Crumbs3a. Traffic 13(8):1170–1185. doi:10.1111/j.1600-0854.2012.01376.x

    PubMed Central  CAS  PubMed  Google Scholar 

  • Harrison OJ, Jin X, Hong S, Bahna F, Ahlsen G, Brasch J, Wu Y, Vendome J, Felsovalyi K, Hampton CM, Troyanovsky RB, Ben-Shaul A, Frank J, Troyanovsky SM, Shapiro L, Honig B (2011) The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins. Structure 19(2):244–256. doi:10.1016/j.str.2010.11.016

    PubMed Central  CAS  PubMed  Google Scholar 

  • Hayashi M, Majumdar A, Li X, Adler J, Sun Z, Vertuani S, Hellberg C, Mellberg S, Koch S, Dimberg A, Koh GY, Dejana E, Belting HG, Affolter M, Thurston G, Holmgren L, Vestweber D, Claesson-Welsh L (2013) VE-PTP regulates VEGFR2 activity in stalk cells to establish endothelial cell polarity and lumen formation. Nat Commun 4:1672. doi:10.1038/ncomms2683

    PubMed Central  PubMed  Google Scholar 

  • Hong S, Troyanovsky RB, Troyanovsky SM (2010) Spontaneous assembly and active disassembly balance adherens junction homeostasis. Proc Natl Acad Sci U S A 107(8):3528–3533. doi:10.1073/pnas.0911027107

    PubMed Central  CAS  PubMed  Google Scholar 

  • Huber MA, Kraut N, Beug H (2005) Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol 17(5):548–558

    CAS  PubMed  Google Scholar 

  • Hurd TW, Fan S, Liu CJ, Kweon HK, Hakansson K, Margolis B (2003a) Phosphorylation-dependent binding of 14-3-3 to the polarity protein Par3 regulates cell polarity in mammalian epithelia. Curr Biol 13(23):2082–2090

    CAS  PubMed  Google Scholar 

  • Hurd TW, Gao L, Roh MH, Macara IG, Margolis B (2003b) Direct interaction of two polarity complexes implicated in epithelial tight junction assembly. Nat Cell Biol 5(2):137–142

    CAS  PubMed  Google Scholar 

  • Hurov JB, Watkins JL, Piwnica-Worms H (2004) Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity. Curr Biol 14(8):736–741

    CAS  PubMed  Google Scholar 

  • Iden S, Misselwitz S, Peddibhotla SS, Tuncay H, Rehder D, Gerke V, Robenek H, Suzuki A, Ebnet K (2012) aPKC phosphorylates JAM-A at Ser285 to promote cell contact maturation and tight junction formation. J Cell Biol 196(5):623–639. doi:10.1083/jcb.201104143

    PubMed Central  CAS  PubMed  Google Scholar 

  • Iden S, Rehder D, August B, Suzuki A, Wolburg-Buchholz K, Wolburg H, Ohno S, Behrens J, Vestweber D, Ebnet K (2006) A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells. EMBO Rep 7(12):1239–1246

    PubMed Central  CAS  PubMed  Google Scholar 

  • Itoh M, Furuse M, Morita K, Kubota K, Saitou M, Tsukita S (1999) Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J Cell Biol 147(6):1351–1363

    PubMed Central  CAS  PubMed  Google Scholar 

  • Jaffe AB, Kaji N, Durgan J, Hall A (2008) Cdc42 controls spindle orientation to position the apical surface during epithelial morphogenesis. J Cell Biol 183(4):625–633

    PubMed Central  CAS  PubMed  Google Scholar 

  • Joberty G, Petersen C, Gao L, Macara IG (2000) The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42. Nat Cell Biol 2(8):531–539

    CAS  PubMed  Google Scholar 

  • Kemphues KJ, Priess JR, Morton DG, Cheng NS (1988) Identification of genes required for cytoplasmic localization in early C. elegans embryos. Cell 52(3):311–320

    CAS  PubMed  Google Scholar 

  • Kitt KN, Nelson WJ (2011) Rapid suppression of activated Rac1 by cadherins and nectins during de novo cell-cell adhesion. PLoS One 6(3):e17841

    PubMed Central  CAS  PubMed  Google Scholar 

  • Kotak S, Busso C, Gonczy P (2012) Cortical dynein is critical for proper spindle positioning in human cells. J Cell Biol 199(1):97–110. doi:10.1083/jcb.201203166

    PubMed Central  CAS  PubMed  Google Scholar 

  • Kotak S, Gonczy P (2013) Mechanisms of spindle positioning: cortical force generators in the limelight. Curr Opin Cell Biol 25(6):741–748. doi:10.1016/j.ceb.2013.07.008

    CAS  PubMed  Google Scholar 

  • Kovacs EM, Ali RG, McCormack AJ, Yap AS (2002) E-cadherin homophilic ligation directly signals through Rac and phosphatidylinositol 3-kinase to regulate adhesive contacts. J Biol Chem 277(8):6708–6718

    CAS  PubMed  Google Scholar 

  • Laan L, Pavin N, Husson J, Romet-Lemonne G, van Duijn M, Lopez MP, Vale RD, Julicher F, Reck-Peterson SL, Dogterom M (2012) Cortical dynein controls microtubule dynamics to generate pulling forces that position microtubule asters. Cell 148(3):502–514

    PubMed Central  CAS  PubMed  Google Scholar 

  • Lemmers C, Michel D, Lane-Guermonprez L, Delgrossi MH, Medina E, Arsanto JP, Le Bivic A (2004) CRB3 binds directly to Par6 and regulates the morphogenesis of the tight junctions in mammalian epithelial cells. Mol Biol Cell 15(3):1324–1333

    PubMed Central  CAS  PubMed  Google Scholar 

  • Lin D, Edwards AS, Fawcett JP, Mbamalu G, Scott JD, Pawson T (2000) A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity. Nat Cell Biol 2(8):540–547

    CAS  PubMed  Google Scholar 

  • Lin X, Shang X, Manorek G, Howell SB (2013) Regulation of the epithelial-mesenchymal transition by claudin-3 and claudin-4. PLoS One 8(6):e67496. doi:10.1371/journal.pone.0067496

    PubMed Central  CAS  PubMed  Google Scholar 

  • Lohia M, Qin Y, Macara IG (2012) The Scribble polarity protein stabilizes E-cadherin/p120-catenin binding and blocks retrieval of E-cadherin to the Golgi. PLoS One 7(11):e51130. doi:10.1371/journal.pone.0051130

    PubMed Central  CAS  PubMed  Google Scholar 

  • Ma L, Young J, Prabhala H, Pan E, Mestdagh P, Muth D, Teruya-Feldstein J, Reinhardt F, Onder TT, Valastyan S, Westermann F, Speleman F, Vandesompele J, Weinberg RA (2010) miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis. Nat Cell Biol 12(3):247–256. doi:10.1038/ncb2024

    PubMed Central  CAS  PubMed  Google Scholar 

  • Macara IG (2004) Parsing the polarity code. Nat Rev Mol Cell Biol 5(3):220–231

    CAS  PubMed  Google Scholar 

  • Makarova O, Roh MH, Liu CJ, Laurinec S, Margolis B (2003) Mammalian Crumbs3 is a small transmembrane protein linked to protein associated with Lin-7 (Pals1). Gene 302(1–2):21–29

    CAS  PubMed  Google Scholar 

  • Mandai K, Nakanishi H, Satoh A, Obaishi H, Wada M, Nishioka H, Itoh M, Mizoguchi A, Aoki T, Fujimoto T, Matsuda Y, Tsukita S, Takai Y (1997) Afadin: a novel actin filament-binding protein with one PDZ domain localized at cadherin-based cell-to-cell adherens junction. J Cell Biol 139(2):517–528, Published erratum appears in J Cell Biol 1997 Nov 17;139(4):1060

    PubMed Central  CAS  PubMed  Google Scholar 

  • McNeill H, Ryan TA, Smith SJ, Nelson WJ (1993) Spatial and temporal dissection of immediate and early events following cadherin-mediated epithelial cell adhesion. J Cell Biol 120(5):1217–1226

    CAS  PubMed  Google Scholar 

  • McSherry EA, McGee SF, Jirstrom K, Doyle EM, Brennan DJ, Landberg G, Dervan PA, Hopkins AM, Gallagher WM (2009) JAM-A expression positively correlates with poor prognosis in breast cancer patients. Int J Cancer 125(6):1343–1351. doi:10.1002/ijc.24498

    CAS  PubMed  Google Scholar 

  • Michel D, Arsanto JP, Massey-Harroche D, Beclin C, Wijnholds J, Le Bivic A (2005) PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells. J Cell Sci 118(Pt 17):4049–4057

    CAS  PubMed  Google Scholar 

  • Mitsushima M, Toyoshima F, Nishida E (2009) Dual role of Cdc42 in spindle orientation control of adherent cells. Mol Cell Biol 29(10):2816–2827

    PubMed Central  CAS  PubMed  Google Scholar 

  • Montero-Balaguer M, Swirsding K, Orsenigo F, Cotelli F, Mione M, Dejana E (2009) Stable vascular connections and remodeling require full expression of VE-cadherin in zebrafish embryos. PLoS One 4(6):e5772

    PubMed Central  PubMed  Google Scholar 

  • Morais-de-Sa E, Mirouse V, St Johnston D (2010) aPKC phosphorylation of Bazooka defines the apical/lateral border in Drosophila epithelial cells. Cell 141(3):509–523

    PubMed Central  CAS  PubMed  Google Scholar 

  • Nagai-Tamai Y, Mizuno K, Hirose T, Suzuki A, Ohno S (2002) Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells. Genes Cells 7(11):1161–1171

    CAS  PubMed  Google Scholar 

  • Nakada C, Ritchie K, Oba Y, Nakamura M, Hotta Y, Iino R, Kasai RS, Yamaguchi K, Fujiwara T, Kusumi A (2003) Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization. Nat Cell Biol 5(7):626–632

    CAS  PubMed  Google Scholar 

  • Nakagawa M, Fukata M, Yamaga M, Itoh N, Kaibuchi K (2001) Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites. J Cell Sci 114(Pt 10):1829–1838

    CAS  PubMed  Google Scholar 

  • Nejsum LN, Nelson WJ (2007) A molecular mechanism directly linking E-cadherin adhesion to initiation of epithelial cell surface polarity. J Cell Biol 178(2):323–335

    PubMed Central  CAS  PubMed  Google Scholar 

  • Nelson WJ (2003) Epithelial cell polarity from the outside looking in. News Physiol Sci 18:143–146

    PubMed Central  CAS  PubMed  Google Scholar 

  • Nielsen JS, McNagny KM (2008) Novel functions of the CD34 family. J Cell Sci 121(Pt 22):3683–3692. doi:10.1242/jcs.037507

    CAS  PubMed  Google Scholar 

  • O’Brien LE, Zegers MM, Mostov KE (2002) Opinion: building epithelial architecture: insights from three-dimensional culture models. Nat Rev Mol Cell Biol 3(7):531–537

    PubMed  Google Scholar 

  • Ozaki C, Obata S, Yamanaka H, Tominaga S, Suzuki ST (2010) The extracellular domains of E- and N-cadherin determine the scattered punctate localization in epithelial cells and the cytoplasmic domains modulate the localization. J Biochem 147(3):415–425. doi:10.1093/jb/mvp192

    CAS  PubMed  Google Scholar 

  • Ozdamar B, Bose R, Barrios-Rodiles M, Wang HR, Zhang Y, Wrana JL (2005) Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity. Science 307(5715):1603–1609

    CAS  PubMed  Google Scholar 

  • Peinado H, Olmeda D, Cano A (2007) Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat Rev Cancer 7(6):415–428

    CAS  PubMed  Google Scholar 

  • Pokutta S, Weis WI (2007) Structure and mechanism of cadherins and catenins in cell-cell contacts. Annu Rev Cell Dev Biol 23:237–261

    CAS  PubMed  Google Scholar 

  • Reinsch S, Karsenti E (1994) Orientation of spindle axis and distribution of plasma membrane proteins during cell division in polarized MDCKII cells. J Cell Biol 126(6):1509–1526

    CAS  PubMed  Google Scholar 

  • Rikitake Y, Mandai K, Takai Y (2012) The role of nectins in different types of cell-cell adhesion. J Cell Sci 125(Pt 16):3713–3722

    CAS  PubMed  Google Scholar 

  • Rodriguez-Boulan E, Paskiet KT, Sabatini DD (1983) Assembly of enveloped viruses in Madin-Darby canine kidney cells: polarized budding from single attached cells and from clusters of cells in suspension. J Cell Biol 96(3):866–874

    CAS  PubMed  Google Scholar 

  • Roh MH, Makarova O, Liu CJ, Shin K, Lee S, Laurinec S, Goyal M, Wiggins R, Margolis B (2002) The Maguk protein, Pals1, functions as an adapter, linking mammalian homologues of Crumbs and Discs Lost. J Cell Biol 157(1):161–172

    PubMed Central  CAS  PubMed  Google Scholar 

  • Sacharidou A, Koh W, Stratman AN, Mayo AM, Fisher KE, Davis GE (2010) Endothelial lumen signaling complexes control 3D matrix-specific tubulogenesis through interdependent Cdc42- and MT1-MMP-mediated events. Blood 115(25):5259–5269

    PubMed Central  CAS  PubMed  Google Scholar 

  • Schneeberger EE, Lynch RD (2004) The tight junction: a multifunctional complex. Am J Physiol Cell Physiol 286(6):C1213–C1228. doi:10.1152/ajpcell.00558.2003

    CAS  PubMed  Google Scholar 

  • Simons K, Fuller SD (1985) Cell surface polarity in epithelia. Annu Rev Cell Biol 1:243–288. doi:10.1146/annurev.cb.01.110185.001331

    CAS  PubMed  Google Scholar 

  • Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, Berry DM, Bonneil E, Thibault P, Schweisguth F, Le Borgne R, McGlade CJ (2007) aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. EMBO J 26(2):468–480

    PubMed Central  CAS  PubMed  Google Scholar 

  • Strilic B, Eglinger J, Krieg M, Zeeb M, Axnick J, Babal P, Muller DJ, Lammert E (2010) Electrostatic cell-surface repulsion initiates lumen formation in developing blood vessels. Curr Biol 20(22):2003–2009. doi:10.1016/j.cub.2010.09.061

    CAS  PubMed  Google Scholar 

  • Strilic B, Kucera T, Eglinger J, Hughes MR, McNagny KM, Tsukita S, Dejana E, Ferrara N, Lammert E (2009) The molecular basis of vascular lumen formation in the developing mouse aorta. Dev Cell 17(4):505–515. doi:10.1016/j.devcel.2009.08.011

    CAS  PubMed  Google Scholar 

  • Suzuki A, Hirata M, Kamimura K, Maniwa R, Yamanaka T, Mizuno K, Kishikawa M, Hirose H, Amano Y, Izumi N, Miwa Y, Ohno S (2004) aPKC acts upstream of PAR-1b in both the establishment and maintenance of mammalian epithelial polarity. Curr Biol 14(16):1425–1435

    CAS  PubMed  Google Scholar 

  • Suzuki A, Ishiyama C, Hashiba K, Shimizu M, Ebnet K, Ohno S (2002) aPKC kinase activity is required for the asymmetric differentiation of the premature junctional complex during epithelial cell polarization. J Cell Sci 115(Pt 18):3565–3573

    CAS  PubMed  Google Scholar 

  • Suzuki A, Ohno S (2006) The PAR-aPKC system: lessons in polarity. J Cell Sci 119(Pt 6):979–987

    CAS  PubMed  Google Scholar 

  • Suzuki A, Yamanaka T, Hirose T, Manabe N, Mizuno K, Shimizu M, Akimoto K, Izumi Y, Ohnishi T, Ohno S (2001) Atypical protein kinase C is involved in the evolutionary conserved PAR protein complex and plays a critical role in establishing epithelia-specific junctional structures. J Cell Biol 152(6):1183–1196

    PubMed Central  CAS  PubMed  Google Scholar 

  • Takahashi K, Nakanishi H, Miyahara M, Mandai K, Satoh K, Satoh A, Nishioka H, Aoki J, Nomoto A, Mizoguchi A, Takai Y (1999) Nectin/PRR: an immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein. J Cell Biol 145(3):539–549

    PubMed Central  CAS  PubMed  Google Scholar 

  • Takai Y, Miyoshi J, Ikeda W, Ogita H (2008) Nectins and nectin-like molecules: roles in contact inhibition of cell movement and proliferation. Nat Rev Mol Cell Biol 9(8):603–615

    CAS  PubMed  Google Scholar 

  • Tepass U, Theres C, Knust E (1990) Crumbs encodes an EGF-like protein expressed on apical membranes of Drosophila epithelial cells and required for organization of epithelia. Cell 61(5):787–799

    CAS  PubMed  Google Scholar 

  • Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K (2011) Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis. Nat Cell Biol 13(2):159–166

    PubMed Central  CAS  PubMed  Google Scholar 

  • Thiery JP, Sleeman JP (2006) Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol 7(2):131–142

    CAS  PubMed  Google Scholar 

  • Thompson BJ, Pichaud F, Roper K (2013) Sticking together the Crumbs – an unexpected function for an old friend. Nat Rev Mol Cell Biol 14(5):307–314. doi:10.1038/nrm3568

    CAS  PubMed  Google Scholar 

  • Toyoshima F, Matsumura S, Morimoto H, Mitsushima M, Nishida E (2007) PtdIns(3,4,5)P3 regulates spindle orientation in adherent cells. Dev Cell 13(6):796–811

    CAS  PubMed  Google Scholar 

  • Toyoshima F, Nishida E (2007) Integrin-mediated adhesion orients the spindle parallel to the substratum in an EB1- and myosin X-dependent manner. EMBO J 26(6):1487–1498

    PubMed Central  CAS  PubMed  Google Scholar 

  • Tsukita S, Furuse M, Itoh M (2001) Multifunctional strands in tight junctions. Nat Rev Mol Cell Biol 2(4):285–293

    CAS  PubMed  Google Scholar 

  • Tsukita S, Yamazaki Y, Katsuno T, Tamura A, Tsukita S (2008) Tight junction-based epithelial microenvironment and cell proliferation. Oncogene 27(55):6930–6938

    CAS  PubMed  Google Scholar 

  • Umeda K, Ikenouchi J, Katahira-Tayama S, Furuse K, Sasaki H, Nakayama M, Matsui T, Tsukita S, Furuse M (2006) ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell 126(4):741–754

    CAS  PubMed  Google Scholar 

  • Van Itallie CM, Anderson JM (2006) Claudins and epithelial paracellular transport. Annu Rev Physiol 68:403–429

    PubMed  Google Scholar 

  • van Meer G, Simons K (1986) The function of tight junctions in maintaining differences in lipid composition between the apical and the basolateral cell surface domains of MDCK cells. EMBO J 5(7):1455–1464

    PubMed Central  PubMed  Google Scholar 

  • Vetter G, Saumet A, Moes M, Vallar L, Le Bechec A, Laurini C, Sabbah M, Arar K, Theillet C, Lecellier CH, Friederich E (2010) miR-661 expression in SNAI1-induced epithelial to mesenchymal transition contributes to breast cancer cell invasion by targeting Nectin-1 and StarD10 messengers. Oncogene 29(31):4436–4448. doi:10.1038/onc.2010.181

    PubMed Central  CAS  PubMed  Google Scholar 

  • Wang AZ, Ojakian GK, Nelson WJ (1990) Steps in the morphogenesis of a polarized epithelium. I. Uncoupling the roles of cell-cell and cell-substratum contact in establishing plasma membrane polarity in multicellular epithelial (MDCK) cysts. J Cell Sci 95(Pt 1):137–151

    PubMed  Google Scholar 

  • Wang Q, Chen XW, Margolis B (2007) PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Mol Biol Cell 18(3):874–885

    PubMed Central  PubMed  Google Scholar 

  • Wang Y, Kaiser MS, Larson JD, Nasevicius A, Clark KJ, Wadman SA, Roberg-Perez SE, Ekker SC, Hackett PB, McGrail M, Essner JJ (2010) Moesin1 and Ve-cadherin are required in endothelial cells during in vivo tubulogenesis. Development 137(18):3119–3128. doi:10.1242/dev.048785

    PubMed Central  CAS  PubMed  Google Scholar 

  • Wells CD, Fawcett JP, Traweger A, Yamanaka Y, Goudreault M, Elder K, Kulkarni S, Gish G, Virag C, Lim C, Colwill K, Starostine A, Metalnikov P, Pawson T (2006) A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells. Cell 125(3):535–548

    CAS  PubMed  Google Scholar 

  • Whiteman EL, Fan S, Harder JL, Walton KD, Liu CJ, Soofi A, Fogg VC, Hershenson MB, Dressler GR, Deutsch GH, Gumucio DL, Margolis B (2013) Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol. doi:10.1128/MCB. 00999-13

    PubMed  Google Scholar 

  • Yamada S, Nelson WJ (2007) Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell-cell adhesion. J Cell Biol 178(3):517–527

    PubMed Central  CAS  PubMed  Google Scholar 

  • Yamanaka T, Horikoshi Y, Izumi N, Suzuki A, Mizuno K, Ohno S (2006) Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity. J Cell Sci 119(Pt 10):2107–2118

    CAS  PubMed  Google Scholar 

  • Yamanaka T, Horikoshi Y, Suzuki A, Sugiyama Y, Kitamura K, Maniwa R, Nagai Y, Yamashita A, Hirose T, Ishikawa H, Ohno S (2001) Par-6 regulates aPKC activity in a novel way and mediates cell-cell contact-induced formation of epithelial junctional complex. Genes Cells 6:721–731

    CAS  PubMed  Google Scholar 

  • Yang J, Weinberg RA (2008) Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev Cell 14(6):818–829. doi:10.1016/j.devcel.2008.05.009

    CAS  PubMed  Google Scholar 

  • Yonemura S, Itoh M, Nagafuchi A, Tsukita S (1995) Cell-to-cell adherens junction formation and actin filament organization: similarities and differences between non-polarized fibroblasts and polarized epithelial cells. J Cell Sci 108(Pt 1):127–142

    CAS  PubMed  Google Scholar 

  • Yu AS, McCarthy KM, Francis SA, McCormack JM, Lai J, Rogers RA, Lynch RD, Schneeberger EE (2005) Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells. Am J Physiol Cell Physiol 288(6):C1231–C1241

    CAS  PubMed  Google Scholar 

  • Zeeb M, Strilic B, Lammert E (2010) Resolving cell-cell junctions: lumen formation in blood vessels. Curr Opin Cell Biol 22(5):626–632. doi:10.1016/j.ceb.2010.07.003

    CAS  PubMed  Google Scholar 

  • Zhang M, Luo W, Huang B, Liu Z, Sun L, Zhang Q, Qiu X, Xu K, Wang E (2013) Overexpression of JAM-A in non-small cell lung cancer correlates with tumor progression. PLoS One 8(11):e79173. doi:10.1371/journal.pone.0079173

    PubMed Central  CAS  PubMed  Google Scholar 

  • Zheng Z, Zhu H, Wan Q, Liu J, Xiao Z, Siderovski DP, Du Q (2010) LGN regulates mitotic spindle orientation during epithelial morphogenesis. J Cell Biol 189(2):275–288

    PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the Deutsche Forschungsgemeinschaft (EB 160/4-1 and 160/4-2) and from the Medical Faculty of the University of Münster (IZKF Eb2/028/09 and Eb2/020/14).

Author information

Authors and Affiliations

  1. Institute-associated Research Group “Cell adhesion and cell polarity,” Institute of Medical Biochemistry, ZMBE, University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany

    Benjamin Franz Brinkmann, Hüseyin Tuncay & Klaus Ebnet

Authors
  1. Benjamin Franz Brinkmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hüseyin Tuncay
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Klaus Ebnet
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Klaus Ebnet .

Editor information

Editors and Affiliations

  1. Institute-associated Research Group “Cell Adhesion and Cell Polarity”, Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation (ZMBE), University Münster, Münster, Nordrhein-Westfalen, Germany

    Klaus Ebnet

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Brinkmann, B.F., Tuncay, H., Ebnet, K. (2015). Homotypic Cell–Cell Interactions and Apicobasal Polarity in Epithelial Cells and Endothelial Cells. In: Ebnet, K. (eds) Cell Polarity 1. Springer, Cham. https://doi.org/10.1007/978-3-319-14463-4_12

Download citation

Publish with us

Policies and ethics

Search

Navigation