Abstract
In Japanese apricot (Prunus mume), six MADS-box genes called DORMANCY-ASSOCIATED MADS-box (PmDAM1–6) are present in a tandem arrangement and are expressed in leaves and buds during the dormant season. Among them, PmDAM1 is up-regulated during the summer when shoot growth is terminated, and it is down-regulated long before dormancy release. To investigate the biological function of PmDAM1, we performed transgenic studies using hybrid poplar (Populus tremula × tremuloides; clone T89). The growth rate of poplar transformed with 35S:PmDAM1 was the same as that of wild-type (WT) poplars. However, 35S:PmDAM1 poplars formed terminal buds earlier than the WT, although terminal buds started to regrow in some 35S:PmDAM1 poplars soon after terminal bud formation. Following artificial cold exposures, the resumption of bud outgrowth in 35S:PmDAM1 poplars was relatively slow compared with the WT when the plants were grown in semi-field conditions that forced bud outgrowth. These results suggested that PmDAM1 caused growth inhibitory effects in transgenic poplar during the transition from growth to growth cessation and from dormancy release to bud outgrowth, when there were limited growth-promoting factors. Based on the data obtained from this poplar transformation study and the seasonal expression pattern of PmDAM1 in Japanese apricot, the involvement of PmDAM1 in the dormancy regulation of Japanese apricot is discussed.
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References
Arora R, Rowland LJ, Tanino K (2003) Induction and release of bud dormancy in woody perennials: a science comes of age. HortScience 38(5):911–921
Bielenberg DG, Li Z, Zhebentyayeva T, Fan S, Reighard GL, Scorza R, Abbott AG (2008) Sequencing and annotation of the evergrowing locus in peach [Prunus persica (L.) Batsch] reveals a cluster of six MADS-box transcription factors as candidate genes for regulation of terminal bud formation. Tree Genet Gen 4(3):495–507
Cooke JK, Eriksson ME, Junttila O (2012) The dynamic nature of bud dormancy in trees: environmental control and molecular mechanisms. Plant Cell Environ 35(10):1707–1728
Habu T, Yamane H, Igarashi K, Hamada K, Yano K, Tao R (2012) 454-pyrosequencing of the transcriptome in leaf and flower buds of Japanese apricot (Prunus mume Sieb. et Zucc.) at different dormant stages. J Jpn Soc Hortic Sci 81(3):239–250
Habu T, Yamane H, Sasaki R, Yano K, Fujii H, Shimizu T et al (2014) Custom microarray analysis for transcript profiling of dormant vegetative buds of Japanese apricot during prolonged chilling exposure. J Jpn Soc Hortic Sci 83(1):1–16
Jimenéz SG, Reighard L, Bielenberg DG (2010) Gene expression of DAM5 and DAM6 is suppressed by chilling temperatures and inversely correlated with bud break rate. Plant Mol Biol 73(1–2):157–167
Mazzitelli L, Hancock RD, Haupt S, Walker PG, Pont SDA, McNicol J et al (2007) Co-ordinated gene expression during phases of dormancy release in raspberry (Rubus idaeus L.) buds. J Exp Bot 58(5):1035–1045
Nakagawa T, Kurose T, Hino T, Tanaka K, Kawamukai M, Niwa Y et al (2007) Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. J Biosci Bioeng 104(1):34–41
Rinne PLH, Welling A, van der Schoot C (2010) Perennial life style of Populus: dormancy cycling and overwintering. In: Jansson S, Bhalerao R, Groover A (eds) Genetics and genomics of Populus. Springer, New York, pp 171–200
Rohde A, Bhalerao RP (2007) Plant dormancy in the perennial context. Trends Plant Sci 12(5):217–223
Rohde A, Storme V, Jorge V, Gaudet M, Vitacolonna N, Fabbrini F et al (2011) Bud set in poplar—genetic dissection of a complex trait in natural and hybrid populations. New Phytol 189(1):106–121
Saito T, Bai S, Ito A, Sakamoto D, Saito T, Ubi BE et al (2013) Expression and genomic structure of the dormancy-associated MADS box genes MADS13 in Japanese pears (Pyrus pyrifolia Nakai) that differ in their chilling requirement for endodormancy release. Tree Physiol 33(6):654–667
Sasaki R, Yamane H, Ooka T, Jotatsu H, Kitamura Y, Akagi T et al (2011) Functional and expressional analyses of PmDAM genes associated with endodormancy in Japanese apricot (Prunus mume). Plant Physiol 157(1):485–497
Tzonev R, Erez A (2003) Inheritance of chilling requirement for dormancy completion in apricot vegetative buds. Acta Hortic 622:429–436
Yamane H (2014) Regulation of bud dormancy and bud break in Japanese apricot (Prunus mume Siebold & Zucc.) and peach [Prunus persica (L.) Batsch]: a summary of recent studies. J Jpn Soc Hortic Sci 83:187–202
Yamane H, Kashiwa Y, Ooka T, Tao R, Yonemori K (2008) Suppression subtractive hybridization and differential screening reveals endodormancy-associated expression of an SVP/AGL24-type MADS-box gene in lateral vegetative buds of Japanese apricot. J Am Soc Hortic Sci 133(5):708–716
Yamane H, Ooka T, Jotatsu H, Hosaka Y, Sasaki R, Tao R (2011a) Expressional regulation of PpDAM5 and PpDAM6, peach (Prunus persica) dormancy-associated MADS-box genes, by low temperature and dormancy-breaking reagent treatment. J Exp Bot 62(10):3481–3488
Yamane H, Ooka T, Jotatsu H, Sasaki R, Tao R (2011b) Expression analysis of PpDAM5 and PpDAM6 during flower bud development in peach (Prunus persica). Sci Hortic 129(4):844–848
Yamane H, Tao R, Ooka T, Jotatsu H, Sasaki R, Yonemori K (2011c) Comparative analyses of dormancy-associated MADS-box genes, PpDAM5 and PpDAM6, in low- and high-chill peaches (Prunus persica L.). J Jpn Soc Hortic Sci 80(3):276–283
Zhang Q, Chen W, Sun L, Zhao F, Huang B, Yang W et al (2012) The genome of Prunus mume. Nat Commun 3:1318
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Yamane, H., Tao, R. (2015). Functional Characterization of Japanese Apricot (Prunus mume) DORMANCY-ASSOCIATED MADS-box1 (PmDAM1), a Paralog of PmDAM6, Using Populus Transformants. In: Anderson, J. (eds) Advances in Plant Dormancy. Springer, Cham. https://doi.org/10.1007/978-3-319-14451-1_8
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DOI: https://doi.org/10.1007/978-3-319-14451-1_8
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