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Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein

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Biotechnology of Extremophiles:

Part of the book series: Grand Challenges in Biology and Biotechnology ((GCBB,volume 1))

Abstract

We recently developed a novel expression system employing the halophilic Archaeon Halobacterium sp. NRC-1 for scaled-up production and nanoparticle-display of antigenic proteins. Here, we have targeted the major human parasite Plasmodium falciparum circumsporozoite protein (CSP), which is of interest for formulation of a protective malaria vaccine. A codon-optimized synthetic gene coding the full-length CSP was inserted downstream of the strong promoter for gvpA, the major gas vesicle nanoparticle protein gene, in the pDRK expression vector, and as a fusion to the gvpC protein in the pSD expression vector for display on the surface of gas vesicle nanoparticles. We found that the pDRK-CSP expression plasmid programmed high-level production of full-length CSP and the pSD-CSP expression plasmid programmed production of a GvpC-CSP fusion protein, for display on gas vesicle nanoparticles. The Halobacterium sp. expression system provides a novel approach and a potentially valuable technical advancement for the production of P. falciparum CSP for malaria vaccine development.

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Acknowledgments

This work was supported by Bill & Melinda Gates Foundation grant OPP1061509, and National Institutes of Health grant R03 AI107634 to SD and National Institutes of Health grant R01 AI056840 to PS. JK was supported by the PKNU Research Abroad Fund CD-2013-0914. We thank Susan Barnes and Folasade Ekulona for technical assistance, Stefanie Trop and Peter Dumoulin for valuable discussions, and Prof. F. Zavala for critical reading of the manuscript.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA

    Wolf T. Pecher, Jong-Myoung Kim, Priya DasSarma, Ram Karan & Shiladitya DasSarma

  2. Institute of Marine and Environmental Technology, University System of Maryland, Baltimore, MD, USA

    Wolf T. Pecher, Jong-Myoung Kim, Priya DasSarma, Ram Karan & Shiladitya DasSarma

  3. College of Arts and Sciences, University of Baltimore, Baltimore, MD, USA

    Wolf T. Pecher

  4. Department of Marine Bio-Materials and Aquaculture, PuKyong National University, Pusan, Republic of Korea

    Jong-Myoung Kim

  5. Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA

    Photini Sinnis

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  1. Wolf T. Pecher
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  2. Jong-Myoung Kim
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  3. Priya DasSarma
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  6. Shiladitya DasSarma
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Correspondence to Shiladitya DasSarma .

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Editors and Affiliations

  1. Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Saint Barthélemy

    Pabulo H Rampelotto

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Wolf T. Pecher, Jong-Myoung Kim, Priya DasSarma, Ram Karan, Photini Sinnis, and Shiladitya DasSarma declare that they have no conflict of interest.

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Pecher, W.T., Kim, JM., DasSarma, P., Karan, R., Sinnis, P., DasSarma, S. (2016). Halobacterium Expression System for Production of Full-Length Plasmodium falciparum Circumsporozoite Protein. In: Rampelotto, P. (eds) Biotechnology of Extremophiles:. Grand Challenges in Biology and Biotechnology, vol 1. Springer, Cham. https://doi.org/10.1007/978-3-319-13521-2_25

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