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Development of DNA Biosensors for Single Nucleotide Polymorphisms

  • Conference paper
VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014

Part of the book series: IFMBE Proceedings ((IFMBE,volume 49))

  • 185 Accesses

Abstract

The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.

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Authors and Affiliations

  1. Instituto Politécnico Nacional, Escuela Superior de Medicina, Laboratorio de Biología Molecular, México, México

    N. M. Pérez-Vielma, G. D. Ibarra-García, M. Gómez-López, M. A. Martínez Godínez & A. Miliar-García

  2. Instituto Politécnico Nacional, Centro de Nanociencias y Micro y Nanotecnologías, México, México

    H. González Martínez

  3. Instituto Politécnico Nacional, Escuela Superior de Medicina, Sección de Investigación y Posgrado, México, México

    C. A. González Díaz & A. V. Vargas Varval

  4. Departamento de Alergia e Inmulogía, Clínica del Hospital Infantil de México Federico Gómez, México, México

    B. E. Del-Río-Navarro

  5. Instituto Politécnico Nacional, Centro de Investigación en Computación, MICROSE, México, México

    V. F. Martínez-Silva & A. Martínez-Rivas

Authors
  1. N. M. Pérez-Vielma
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  2. G. D. Ibarra-García
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  3. M. Gómez-López
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  4. M. A. Martínez Godínez
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  5. H. González Martínez
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  6. C. A. González Díaz
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  7. B. E. Del-Río-Navarro
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  8. V. F. Martínez-Silva
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  9. A. Martínez-Rivas
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  10. A. V. Vargas Varval
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  11. A. Miliar-García
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Corresponding author

Correspondence to N. M. Pérez-Vielma .

Editor information

Editors and Affiliations

  1. Universidad Nacional de Entre Ríos, Facultad de Ingeniería - UNER, Entre Ríos, Argentina

    Ariel Braidot

  2. Universidad Nacional de Entre Ríos, Entre Ríos, Argentina

    Alejandro Hadad

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© 2015 Springer International Publishing Switzerland

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Pérez-Vielma, N.M. et al. (2015). Development of DNA Biosensors for Single Nucleotide Polymorphisms. In: Braidot, A., Hadad, A. (eds) VI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014. IFMBE Proceedings, vol 49. Springer, Cham. https://doi.org/10.1007/978-3-319-13117-7_15

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  • DOI: https://doi.org/10.1007/978-3-319-13117-7_15

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-13116-0

  • Online ISBN: 978-3-319-13117-7

  • eBook Packages: EngineeringEngineering (R0)

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