Abstract
The structure and the interaction of proteins are two important information that, in addition to their expression, characterize proteins. The accuracy and the resolution of current mass spectrometers allow the mass measurement of large molecules with high precision. Nevertheless, mass values are not sufficient to provide structure information. In the case of protein interaction, it could provide data on the stoichiometry of the interaction, but no structural details. Several methods have been developed to use mass data and mass spectrometry to decipher the three-dimensional structure of proteins and complexes. However, it is important to note that mass spectrometry completes existing techniques such as crystallography or NMR, allowing the analysis of different proteins or complexes. This chapter presents approaches used in the analysis of proteins structures and complexes involving proteins.
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Pottiez, G. (2015). Can Mass Spectrometry Help Determine Proteins Structure and Interactions?. In: Mass Spectrometry: Developmental Approaches to Answer Biological Questions. SpringerBriefs in Bioengineering. Springer, Cham. https://doi.org/10.1007/978-3-319-13087-3_4
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DOI: https://doi.org/10.1007/978-3-319-13087-3_4
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