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Heterotrophic Bacteria Producing Polyhydroxyalkanoates: A Biodegradable Polymer

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Abstract

Polyhydroxyalkanoates (PHAs), a class of naturally occurring, optically active, aliphatic biopolyesters are gaining tremendous attention as they are the potential substitute to the nonbiodegradable petrochemical-based synthetic plastics. Microorganisms synthesize PHA in the form of intracellular, spherical, nano-sized inclusions, the size and number of which vary depending upon the bacteria. They are potentially useful in industries, with the major application of PHAs being in the medical field, especially in tissue engineering. In recent years, Gram-positive organisms such as Bacillus and Streptomyces species have been recognized as potential strains for commercial scale PHA production as lipopolysaccharide is one of the major contaminants known to copurify when extracted from Gram-negative bacteria.

Isolation of bacteria from diverse coastal and marine arenas carried out to obtain PHA producers revealed the highest heterotrophic bacterial load in the mangrove area. However, the distribution of PHA producers was the highest in the nutrient-limited marine sediment samples. Based on the intensity as well as the duration of maximum PHA production on glucose, 11 Gram-positive, rod-shaped sporulating isolates were selected for further studies. These isolates belonged to the genus Bacillus, with the majority being Bacillus megaterium. Nile blue A staining of the isolates revealed the presence of intracellular PHA granules which exhibited bright orange-red fluorescence when viewed under fluorescent light. Further, characterization of the polymer extracted from these bacteria using Fourier transform infrared (FTIR) spectroscopy confirmed the aliphatic nature of the PHA polymer. Different strains of Bacillus species obtained in the present study can be exploited for the production of PHAs for biomedical applications.

Keywords

Mangroves Biodegradable polymer Polyhydroxyalkanoates Nile blue A Bacillus megaterium 

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Department of Biological SciencesBirla Institute of Technology and Science-PilaniZuarinagarIndia

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