Response of Alkaliphilic Bacteria to Aromatic Amines



Aromatic amines are reported to be one of the most prominent environmental pollutants being released in the form of effluents or pesticides and are well known for their properties as potential mutagens as well as carcinogens. Aniline is an aromatic amine and is largely used as an intermediate in the synthesis of many synthetic organic compounds. Several azo dyes and nitroamine compounds on biotransformation produce aromatic amines like p-phenylenediamine, benzedene or aniline which can damage an ecosystem depending on the input quantities. Presence of such bioenvironmental amino aromatic contaminants is a serious threat and a danger to human health and other aquatic organisms as these combinations tend to be very resistant to degradation.

In the present study, attempts were made to investigate the response of alkaliphilic bacteria isolated from marine ecosystems of Goa to N, N-Diemthyl-1-naphthylamine (NND) and aniline used as model aromatic amines under alkaline conditions. Two potential halotolerant alkaliphiles, capable of tolerating high concentration of NND and aniline were isolated. Halomonas pacifica strain NK2 produced a biosurfactant in response to NND and Halomonas campanieinsis strain NRS-01 showed the production of a red-coloured steroid-like compound in the presence of high concentrations of aniline. Formation of the red compound was preceded with the aggregation of cells and production of a blue fluorescent protein, both observed only in the presence of cells, indicating the role of NRS-01 in the formation of these products. Such biotransformation products can be important bioindicators for the early detection of hydrocarbon pollution.


Extremophiles Alkaliphiles Halomonas N, N-Dimethyl-1-Naphthylamine Aniline Biosurfactant Red compound 


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© Springer International Publishing Switzerland 2015

Authors and Affiliations

  1. 1.Fomento ResourcesPanjimIndia

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