Skip to main content
SpringerLink
Log in

MicroRNAs in Obesity and Metabolism

  • Chapter
  • First Online:
Molecular Mechanisms Underpinning the Development of Obesity

  • 762 Accesses

Abstract

MicroRNAs (miRNAs; miRs) are small, endogenous, non-coding RNAs that play an important regulatory role in cell physiology because of their inhibitory effect in gene expression. In most cases, the 21-25 nucleotide miRNA binds to the 3’ untranslated region (3’ UTR) of target mRNA based on nucleotide complementary, and initiates the cleavage or translational repression of mRNA transcripts. MiRNAs are involved in a broad range of biological functions and are expressed in a tissue specific manner. A growing body of evidence shows that miRNAs are important regulators of several metabolic processes including adipocytes differentiation, insulin production and secretion, glucose metabolism and insulin resistance. More importantly, it has been reported that deregulation of the miRNAs pathway is associated to obesity and related conditions such as diabetes. Accordingly, global miRNAs profiling studies in rodents and humans show that obesity, high-fat diet and diabetes lead to alterations in the expression pattern of specific miRNAs in different metabolic tissues namely, adipose tissue, muscle, liver and pancreas. Moreover, changes in miRNAs and target mRNAs levels result in defective or distorted cellular mechanisms that may contribute to the pathogenesis of obesity and related complications. In this context, miRNA modulation emerges as a possible therapeutic option to reestablish the regulatory balance in biological pathways affect by obesity. Another interesting concept is the utilization of plasma miRNAs levels as biomarkers for obesity and diabetes. This chapter will focus on the role of miRNAs and their targets in the cellular mechanisms regulating metabolism and the involvement of miRNAs in the pathogenesis of obesity and related diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116(2):281–297

    Article  CAS  PubMed  Google Scholar 

  2. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T (2001) Identification of novel genes coding for small expressed RNAs. Science 294(5543):853–858

    Article  CAS  PubMed  Google Scholar 

  3. Guo H, Ingolia NT, Weissman JS, Bartel DP (2010) Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 466(7308):835–840

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75(5):843–854

    Article  CAS  PubMed  Google Scholar 

  5. Wightman B, Ha I, Ruvkun G (1993) Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell 75(5):855–862

    Article  CAS  PubMed  Google Scholar 

  6. Rottiers V, Naar AM (2012) MicroRNAs in metabolism and metabolic disorders. Nat Rev Mol Cell Biol 13(4):239–250

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136(2):215–233

    Google Scholar 

  8. Doench JG, Sharp PA (2004) Specificity of microRNA target selection in translational repression. Genes Dev 18(5):504–511

    Google Scholar 

  9. Baek D, Villén J, Shin C, Camargo FD, Gygi SP, Bartel DP (2008) The impact of microRNAs on protein output. Nature 455(7209):64–71

    Google Scholar 

  10. Cai X, Hagedorn CH, Cullen BR (2004) Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10(12):1957–1966

    Google Scholar 

  11. Lee Y, Kim M, Han J, Yeom KH, Lee S, Baek SH, Kim VN (2004) MicroRNA genes are transcribed by RNA polymerase II. EMBO J 23(20):4051–4060

    Google Scholar 

  12. Gregory RI, Yan KP, Amuthan G, Chendrimada T, Doratotaj B, Cooch N, Shiekhattar R (2004) The Microprocessor complex mediates the genesis of microRNAs. Nature 432(7014):235–240

    Google Scholar 

  13. Lee Y, Ahn C, Han J, Choi H, Kim J, Yim J, Lee J, Provost P, Rådmark O, Kim S, Kim VN (2003) The nuclear RNase III Drosha initiates microRNA processing. Nature 425(6956):415–419

    Google Scholar 

  14. Haase AD, Jaskiewicz L, Zhang H, Lainé S, Sack R, Gatignol A, Filipowicz W (2005) TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing. EMBO Rep 6(10):961–967

    Google Scholar 

  15. Hutvagner G, McLachlan J, Pasquinelli AE, Bálint E, Tuschl T, Zamore PD (2001) A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293(5531):834–838

    Google Scholar 

  16. Chendrimada TP, Gregory RI, Kumaraswamy E, Norman J, Cooch N, Nishikura K, Shiekhattar R (2005) TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. Nature 436(7051):740–744

    Google Scholar 

  17. Huntzinger E, Izaurralde E (2011) Gene silencing by microRNAs: contributions of translational repression and mRNA decay. Nat Rev Genet 12(2):99–110

    Google Scholar 

  18. Ku G, McManus MT (2008) Behind the scenes of a small RNA gene-silencing pathway. Hum Gene Ther 19(1):17–26

    Google Scholar 

  19. Tang QQ, Lane MD (2012) Adipogenesis: from stem cell to adipocyte. Annu Rev Biochem 81:715–736

    Google Scholar 

  20. Oskowitz AZ, Lu J, Penfornis P, Ylostalo J, McBride J, Flemington EK, Prockop DJ, Pochampally R (2008) Human multipotent stromal cells from bone marrow and microRNA: regulation of differentiation and leukemia inhibitory factor expression. Proc Natl Acad Sci U S A 105(47):18372–18377

    Google Scholar 

  21. Esau C, Kang X, Peralta E, Hanson E, Marcusson EG, Ravichandran LV, Sun Y et al (2004) MicroRNA-143 regulates adipocyte differentiation. J Biol Chem 279(50):52361–52365

    Google Scholar 

  22. Kajimoto K, Naraba H, Iwai N (2006) MicroRNA and 3T3-L1 pre-adipocyte differentiation. RNA 12(9):1626–1632

    Google Scholar 

  23. Gerin I, Bommer GT, McCoin CS, Sousa KM, Krishnan V, MacDougald OA (2010) Roles for miRNA-378/378* in adipocyte gene expression and lipogenesis. Am J Physiol Endocrinol Metab 299(2):E198–206

    Google Scholar 

  24. Sun F, Wang J, Pan Q, Yu Y, Zhang Y, Wan Y, Wang J, Li X, Hong A (2009) Characterization of function and regulation of miR-24-1 and miR-31. Biochem Biophys Res Commun 380(3):660–665

    Google Scholar 

  25. Chen L, Cui J, Hou J, Long J, Li C, Liu L (2014) A novel negative regulator of adipogenesis: microRNA-363. Stem Cells 32(2):510–520

    Google Scholar 

  26. Lin Q, Gao Z, Alarcon RM, Ye J, Yun Z (2009) A role of miR-27 in the regulation of adipogenesis. FEBS J 276(8):2348–2358

    Google Scholar 

  27. Xie H, Lim B, Lodish HF (2009) MicroRNAs induced during adipogenesis that accelerate fat cell development are downregulated in obesity. Diabetes 58(5):1050–1057

    Google Scholar 

  28. Kim SY, Kim AY, Lee HW, Son YH, Lee GY, Lee JW, Lee YS, Kim JB (2010) miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARgamma expression. Biochem Biophys Res Commun 392(3):323–328

    Google Scholar 

  29. Karbiener M, Fischer C, Nowitsch S, Opriessnig P, Papak C, Ailhaud G, Dani C, Amri EZ, Scheideler M (2009) microRNA miR-27b impairs human adipocyte differentiation and targets PPARgamma. Biochem Biophys Res Commun 390(2):247–251

    Google Scholar 

  30. Wang Q, Li YC, Wang J, Kong J, Qi Y, Quigg RJ, Li X (2008) miR-17-92 cluster accelerates adipocyte differentiation by negatively regulating tumor-suppressor Rb2/p130. Proc Natl Acad Sci U S A 105(8):2889–2894

    Google Scholar 

  31. Ortega FJ, Moreno-Navarrete JM, Pardo G, Sabater M, Hummel M, Ferrer A, Rodriguez-Hermosa JI et al (2010) MiRNA expression profile of human subcutaneous adipose and during adipocyte differentiation. PLoS ONE 5(2):e9022

    Google Scholar 

  32. Qin L, Chen Y, Niu Y, Chen W, Wang Q, Xiao S, Li A et al (2010) A deep investigation into the adipogenesis mechanism: profile of microRNAs regulating adipogenesis by modulating the canonical Wnt/beta-catenin signaling pathway. BMC Genomics 11:e320

    Google Scholar 

  33. Zaragosi LE, Wdziekonski B, Brigand KL, Villageois P, Mari B, Waldmann R, Dani C, Barbry P (2011) Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis. Genome Biol 12(7):R64

    Google Scholar 

  34. Kim YJ, Hwang SH, Cho HH, Shin KK, Bae YC, Jung JS (2012) MicroRNA 21 regulates the proliferation of human adipose tissue-derived mesenchymal stem cells and high-fat diet-induced obesity alters microRNA 21 expression in white adipose tissues. J Cell Physiol 227(1):183–193

    Google Scholar 

  35. Karbiener M, Neuhold C, Opriessnig P, Prokesch A, Bogner-Strauss JG, Scheideler M (2011) MicroRNA-30c promotes human adipocyte differentiation and co-represses PAI-1 and ALK2. RNA Biol 8(5):850–860

    Google Scholar 

  36. Nakanishi N, Nakagawa Y, Tokushige N, Aoki N, Matsuzaka T, Ishii K, Yahagi N et al (2009) The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice. Biochem Biophys Res Commun 385(4):492–496

    Google Scholar 

  37. Chartoumpekis DV, Zaravinos A, Ziros PG, Iskrenova RP, Psyrogiannis AI, Kyriazopoulou VE, Habeos IG (2012) Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice. PLoS ONE 7(4):e34872

    Google Scholar 

  38. Martinelli R, Nardelli C, Pilone V, Buonomo T, Liguori R, Castanò I, Buono P et al (2010) miR-519d overexpression is associated with human obesity. Obesity (Silver Spring) 18(11):2170–2176

    Google Scholar 

  39. Kloting N, Berthold S, Kovacs P, Schön MR, Fasshauer M, Ruschke K, Stumvoll M, Blüher M (2009) MicroRNA expression in human omental and subcutaneous adipose tissue. PLoS ONE 4(3):e4699

    Google Scholar 

  40. Heneghan HM, Miller N, McAnena OJ, O'Brien T, Kerin MJ (2011) Differential miRNA expression in omental adipose tissue and in the circulation of obese patients identifies novel metabolic biomarkers. J Clin Endocrinol Metab 96(5):e846 − 50

    Google Scholar 

  41. He Z, Yu J, Zhou C, Ren G, Cong P, Mo D, Chen Y, Liu X (2013) MiR-143 is not essential for adipose development as revealed by in vivo antisense targeting. Biotechnol Lett 35(4):499–507

    Google Scholar 

  42. Takanabe R, Ono K, Abe Y, Takaya T, Horie T, Wada H, Kita T, Satoh N, Shimatsu A, Hasegawa K (2008) Up-regulated expression of microRNA-143 in association with obesity in adipose tissue of mice fed high-fat diet. Biochem Biophys Res Commun 376(4):728–732

    Google Scholar 

  43. Jordan SD, Krüger M, Willmes DM, Redemann N, Wunderlich FT, Brönneke HS, Merkwirth C et al (2011) Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 13(4):434–446

    Google Scholar 

  44. Kim YJ, Hwang SJ, Bae YC, Jung JS (2009) MiR-21 regulates adipogenic differentiation through the modulation of TGF-beta signaling in mesenchymal stem cells derived from human adipose tissue. Stem Cells 27(12):3093–3102

    Google Scholar 

  45. Ling HY, Wen GB, Feng SD, Tuo QH, Ou HS, Yao CH, Zhu BY et al (2011) MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling. Clin Exp Pharmacol Physiol 38(4):239–246

    Google Scholar 

  46. Kennell JA, Gerin I, MacDougald OA, Cadigan KM (2008) The microRNA miR-8 is a conserved negative regulator of Wnt signaling. Proc Natl Acad Sci U S A 105(40):15417–15422

    Google Scholar 

  47. Sun T, Fu M, Bookout AL, Kliewer SA, Mangelsdorf DJ (2009) MicroRNA let-7 regulates 3T3-L1 adipogenesis. Mol Endocrinol 23(6):925–931

    Google Scholar 

  48. Yang Z, Bian C, Zhou H, Huang S, Wang S, Liao L, Zhao RC (2011) MicroRNA hsa-miR-138 inhibits adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells through adenovirus EID-1. Stem Cells Dev 20(2):259–267

    Google Scholar 

  49. Bussing I, Slack FJ, Grosshans H (2008) let-7 microRNAs in development, stem cells and cancer. Trends Mol Med 14(9):400–409

    Google Scholar 

  50. Anand A, Chada K (2000) In vivo modulation of Hmgic reduces obesity. Nat Genet 24(4):377–380

    Google Scholar 

  51. Frost RJ, Olson EN (2011) Control of glucose homeostasis and insulin sensitivity by the Let-7 family of microRNAs. Proc Natl Acad Sci U S A 108(52):21075–21080

    Google Scholar 

  52. Kinoshita M, Ono K, Horie T, Nagao K, Nishi H, Kuwabara Y, Takanabe-Mori R, Hasegawa K, Kita T, Kimura T (2010) Regulation of adipocyte differentiation by activation of serotonin (5-HT) receptors 5-HT2AR and 5-HT2CR and involvement of microRNA-448-mediated repression of KLF5. Mol Endocrinol 24(10):1978–1987

    Google Scholar 

  53. Andersen DC, Jensen CH, Schneider M, Nossent AY, Eskildsen T, Hansen JL, Teisner B, Sheikh SP (2010) MicroRNA-15a fine-tunes the level of Delta-like 1 homolog (DLK1) in proliferating 3T3-L1 preadipocytes. Exp Cell Res 316(10):1681–1691

    Google Scholar 

  54. Wellen KE, Hotamisligil GS (2003) Obesity-induced inflammatory changes in adipose tissue. J Clin Invest 112(12):1785–1788

    Google Scholar 

  55. Cawthorn WP, Sethi JK (2008) TNF-alpha and adipocyte biology. FEBS Lett 582(1):117–131

    Google Scholar 

  56. Wang YC, Li Y, Wang XY, Zhang D, Zhang H, Wu Q, He YQ et al (2013) Circulating miR-130b mediates metabolic crosstalk between fat and muscle in overweight/obesity. Diabetologia 56(10):2275–2285

    Google Scholar 

  57. Parra P, Serra F, Palou A (2010) Expression of adipose microRNAs is sensitive to dietary conjugated linoleic acid treatment in mice. PLoS ONE 5(9):e13005

    Google Scholar 

  58. Karolina DS, Armugam A, Tavintharan S, Wong MT, Lim SC, Sum CF, Jeyaseelan K (2011) MicroRNA 144 impaired insulin signaling by inhibiting the expression of insulin receptor substrate 1 in type 2 diabetes mellitus. PLoS ONE 6(8):e22839

    Google Scholar 

  59. Plaisance V, Abderrahmani A, Perret-Menoud V, Jacquemin P, Lemaigre F, Regazzi R (2006) MicroRNA-9 controls the expression of Granuphilin/Slp4 and the secretory response of insulin-producing cells. J Biol Chem 281(37):26932–26942

    Google Scholar 

  60. Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S et al (2004) A pancreatic islet-specific microRNA regulates insulin secretion. Nature 432(7014):226–230

    Google Scholar 

  61. Tang X, Muniappan L, Tang G, Ozcan S (2009) Identification of glucose-regulated miRNAs from pancreatic {beta} cells reveals a role for miR-30d in insulin transcription. RNA 15(2):287–293

    Google Scholar 

  62. Zhu H, Shyh-Chang N, Segrè AV, Shinoda G, Shah SP, Einhorn WS, Takeuchi A et al (2011) The Lin28/let-7 axis regulates glucose metabolism. Cell 147(1):81–94

    Google Scholar 

  63. Pandey AK, Verma G, Vig S, Srivastava S, Srivastava AK, Datta M (2011) miR-29a levels are elevated in the db/db mice liver and its overexpression leads to attenuation of insulin action on PEPCK gene expression in HepG2 cells. Mol Cell Endocrinol 332(1-2):125–133

    Google Scholar 

  64. Trajkovski M, Hausser J, Soutschek J, Bhat B, Akin A, Zavolan M, Heim MH, Stoffel M (2011) MicroRNAs 103 and 107 regulate insulin sensitivity. Nature 474(7353):649–653

    Google Scholar 

  65. Esguerra JL, Bolmeson C, Cilio CM, Eliasson L (2011) Differential glucose-regulation of microRNAs in pancreatic islets of non-obese type 2 diabetes model Goto-Kakizaki rat. PLoS ONE 6(4):e18613

    Google Scholar 

  66. Lovis P, Roggli E, Laybutt DR, Gattesco S, Yang JY, Widmann C, Abderrahmani A, Regazzi R (2008) Alterations in microRNA expression contribute to fatty acid-induced pancreatic beta-cell dysfunction. Diabetes 57(10):2728–2736

    Google Scholar 

  67. Zhu Y, You W, Wang H, Li Y, Qiao N, Shi Y, Zhang C, Bleich D, Han X (2013) MicroRNA-24/MODY gene regulatory pathway mediates pancreatic beta-cell dysfunction. Diabetes 62(9):3194–3206

    Google Scholar 

  68. Poy MN, Hausser J, Trajkovski M, Braun M, Collins S, Rorsman P, Zavolan M, Stoffel M (2009) miR-375 maintains normal pancreatic alpha- and beta-cell mass. Proc Natl Acad Sci USA 106(14):5813–5818

    Google Scholar 

  69. Sun LL, Jiang BG, Li WT, Zou JJ, Shi YQ, Liu ZM (2011) MicroRNA-15a positively regulates insulin synthesis by inhibiting uncoupling protein-2 expression. Diabetes Res Clin Pract 91(1):94–100

    Google Scholar 

  70. Fred RG, Bang-Berthelsen CH, Mandrup-Poulsen T, Grunnet LG, Welsh N (2010) High glucose suppresses human islet insulin biosynthesis by inducing miR-133a leading to decreased polypyrimidine tract binding protein-expression. PLoS ONE 5(5):e10843

    Google Scholar 

  71. Lovis P, Gattesco S, Regazzi R (2008) Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by microRNAs. Biol Chem 389(3):305–312

    Google Scholar 

  72. Ramachandran D, Roy U, Garg S, Ghosh S, Pathak S, Kolthur-Seetharam U (2011) Sirt1 and mir-9 expression is regulated during glucose-stimulated insulin secretion in pancreatic beta-islets. FEBS J 278(7):1167–1174

    Google Scholar 

  73. Baroukh N, Ravier MA, Loder MK, Hill EV, Bounacer A, Scharfmann R, Rutter GA, Van Obberghen E (2007) MicroRNA-124a regulates Foxa2 expression and intracellular signaling in pancreatic beta-cell lines. J Biol Chem 282(27):19575–19588

    Google Scholar 

  74. Aspinwall CA, Qian WJ, Roper MG, Kulkarni RN, Kahn CR, Kennedy RT (2000) Roles of insulin receptor substrate-1, phosphatidylinositol 3-kinase, and release of intracellular Ca2+ stores in insulin-stimulated insulin secretion in beta -cells. J Biol Chem 275(29):22331–22338

    Google Scholar 

  75. El Ouaamari A, Baroukh N, Martens GA, Lebrun P, Pipeleers D, van Obberghen E (2008) miR-375 targets 3’-phosphoinositide-dependent protein kinase-1 and regulates glucose-induced biological responses in pancreatic beta-cells. Diabetes 57(10):2708–2717

    Google Scholar 

  76. Michael MD, Kulkarni RN, Postic C, Previs SF, Shulman GI, Magnuson MA, Kahn CR (2000) Loss of insulin signaling in hepatocytes leads to severe insulin resistance and progressive hepatic dysfunction. Mol Cell 6(1):87–97

    Google Scholar 

  77. Burks DJ MF (2001) White, IRS proteins and beta-cell function. Diabetes 50(Suppl 1):S140–S145

    Google Scholar 

  78. Shepherd PR, Withers DJ, Siddle K (1998) Phosphoinositide 3-kinase: the key switch mechanism in insulin signalling. Biochem J 333(Pt 3):471–490

    Google Scholar 

  79. Herrera BM, Lockstone HE, Taylor JM, Ria M, Barrett A, Collins S, Kaisaki P et al (2010) Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes. Diabetologia 53(6):1099–1109

    Google Scholar 

  80. Herrera BM, Lockstone HE, Taylor JM, Wills QF, Kaisaki PJ, Barrett A, Camps C et al (2009) MicroRNA-125a is over-expressed in insulin target tissues in a spontaneous rat model of type 2 Diabetes. BMC Med Genomics 2:54

    Google Scholar 

  81. He A, Zhu L, Gupta N, Chang Y, Fang F (2007) Overexpression of micro ribonucleic acid 29, highly up-regulated in diabetic rats, leads to insulin resistance in 3T3-L1 adipocytes. Mol Endocrinol 21(11):2785–2794

    Google Scholar 

  82. Kornfeld JW, Baitzel C, Könner AC, Nicholls HT, Vogt MC, Herrmanns K, Scheja L et al (2013) Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b. Nature 494(7435):111–115

    Google Scholar 

  83. Ryu HS, Park SY, Ma D, Zhang J, Lee W (2011) The induction of microRNA targeting IRS-1 is involved in the development of insulin resistance under conditions of mitochondrial dysfunction in hepatocytes. PLoS ONE 6(3):e17343

    Google Scholar 

  84. Davalos A, Goedeke L, Smibert P, Ramírez CM, Warrier NP, Andreo U, Cirera-Salinas D et al (2011) miR-33a/b contribute to the regulation of fatty acid metabolism and insulin signaling. Proc Natl Acad Sci U S A 108(22):9232–9237

    Google Scholar 

  85. Roush S, Slack FJ (2008) The let-7 family of microRNAs. Trends Cell Biol 18(10):505–516

    Google Scholar 

  86. Xu J, Wong C (2008) A computational screen for mouse signaling pathways targeted by microRNA clusters. RNA 14(7):1276–1283

    Google Scholar 

  87. Hunter MP, Ismail N, Zhang X, Aguda BD, Lee EJ, Yu L, Xiao T et al (2008) Detection of microRNA expression in human peripheral blood microvesicles. PLoS ONE 3(11):e3694

    Google Scholar 

  88. Turchinovich A, Weiz L, Langheinz A, Burwinkel B (2011) Characterization of extracellular circulating microRNA. Nucleic Acids Res 39(16):7223–7233

    Google Scholar 

  89. Kosaka N, Iguchi H, Yoshioka Y, Takeshita F, Matsuki Y, Ochiya T (2010) Secretory mechanisms and intercellular transfer of microRNAs in living cells. J Biol Chem 285(23):17442–17452

    Google Scholar 

  90. Vickers KC, Palmisano BT, Shoucri BM, Shamburek RD, Remaley AT (2011) MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol 13(4):423–433

    Google Scholar 

  91. Laterza OF, Lim L, Garrett-Engele PW, Vlasakova K, Muniappa N, Tanaka WK, Johnson JM et al (2009) Plasma MicroRNAs as sensitive and specific biomarkers of tissue injury. Clin Chem 55(11):1977–1983

    Google Scholar 

  92. Fichtlscherer S, De Rosa S, Fox H, Schwietz T, Fischer A, Liebetrau C, Weber M et al (2010) Circulating microRNAs in patients with coronary artery disease. Circ Res 107(5):677–684

    Google Scholar 

  93. Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, Guo J et al (2008) Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res 18(10):997–1006

    Google Scholar 

  94. Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A et al (2008) Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A 105(30):10513–10518

    Google Scholar 

  95. Cermelli S, Ruggieri A, Marrero JA, Ioannou GN, Beretta L (2011) Circulating microRNAs in patients with chronic hepatitis C and non-alcoholic fatty liver disease. PLoS ONE 6(8):e23937

    Google Scholar 

  96. Weiland M, Gao XH, Zhou L, Mi QS (2012) Small RNAs have a large impact: circulating microRNAs as biomarkers for human diseases. RNA Biol 9(6):850–859

    Google Scholar 

  97. Zampetaki A, Kiechl S, Drozdov I, Willeit P, Mayr U, Prokopi M, Mayr A et al (2010) Plasma microRNA profiling reveals loss of endothelial miR-126 and other microRNAs in type 2 diabetes. Circ Res 107(6):810–817

    Google Scholar 

  98. Krutzfeldt J, Rajewsky N, Braich R, Rajeev KG, Tuschl T, Manoharan M, Stoffel M (2005) Silencing of microRNAs in vivo with ‘antagomirs’. Nature 438(7068):685–689 (ADDIN EN.REFLIST)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center For Neuroscience and Cell Biology & Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal

    Lígia Sousa-Ferreira

  2. CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal

    Luís Pereira de Almeida & Cláudia Cavadas

Authors
  1. Lígia Sousa-Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luís Pereira de Almeida
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cláudia Cavadas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cláudia Cavadas .

Editor information

Editors and Affiliations

  1. Cntr for Neurosciences and Cell Biology University of Coimbra, Coimbra, Portugal

    Clévio Nóbrega

  2. Nutrition and Gastroenterology Department, Armand-Trousseau Hospital, Valencia, Spain

    Raquel Rodriguez-López

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Sousa-Ferreira, L., Pereira de Almeida, L., Cavadas, C. (2014). MicroRNAs in Obesity and Metabolism. In: Nóbrega, C., Rodriguez-López, R. (eds) Molecular Mechanisms Underpinning the Development of Obesity. Springer, Cham. https://doi.org/10.1007/978-3-319-12766-8_9

Download citation

Publish with us

Policies and ethics

Search

Navigation