Abstract
Brain tissue required for staging procedures (Braak et al. 2006a). The tissue on one side of a cut can be used for conventional paraffin embedding (70–100 μm sections; see Feldengut et al. 2013), and that on the other side for polyethylene glycol (PEG) embedding (100–400 μm sections). A section thickness of 70–100 μm meets the demands of low power light (stereo)microscopy and greatly facilitates recognition of the lesional distribution pattern (Fig. 11.1).
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References
Braak H, Alafuzoff I, Arzberger T et al (2006a) Staging of Alzheimer’s disease-associated neurofibrillary pathology using paraffin sections and immunohistochemistry. Acta Neuropathol 112:389–404
Feldengut S, Del Tredici K, Braak H (2013) Paraffin sections of 70-100 μm: a novel technique and its benefits for studying the nervous system. J Neurosci Method 215:241–244
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Braak, H., Del Tredici, K. (2015). Technical Addendum. In: Neuroanatomy and Pathology of Sporadic Alzheimer's Disease. Advances in Anatomy, Embryology and Cell Biology, vol 215. Springer, Cham. https://doi.org/10.1007/978-3-319-12679-1_11
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DOI: https://doi.org/10.1007/978-3-319-12679-1_11
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