Abstract
This chapter presents the recombinant stem bromelain cloning procedure followed by its characterization and finally upstream processing. Most of the procedures had patents filed in Malaysia, Europe and the United State of America with application numbers PI 20095434, 10015711.4, and 12968766, respectively. Portions of the experimental procedures were also presented in our publication (Amid et al., Process Biochem 46:2232–2239, 2011). The entire cloning process, characterization and the upstream processing of the recombinant bromelain production are briefly explained.
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References
Amid A, Ismail NA, Yusof F, Salleh HM (2011) Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus. Process Biochem 46:2232–2239
Nicholl DST (2009) An introduction to genetic engineering, 3rd edn. Cambridge University Press, Cambridge
Koller CA, Kohli V (1993) Purification of genomic DNA using heparin to remove nuclear proteins. Nucleic Acids Res 21:2952
Vanhanen M, Tuomi T, Hokkanen H, Tupasela O, Tuomainen A, Holmberg PC et al (1996) Enzyme exposure and enzyme sensitisation in the baking industry. Occup Environ Med 53:670–676.
Liu ZB, Weis R, Glieder A (2004) Enzymes from higher eukaryotes for industrial biocatalysis. Food Technol Biotech 42:237–249
Pandey A, Benjamin S, Soccol CR, Nigam P, Krieger N, Soccol VT (1999) The realm of microbial lipases in biotechnology. Biotechnol Appl Bioc 29:119–131
Pillai S, Oresajo C, Hayward J (2005) Ultraviolet radiation and skin aging: roles of reactive oxygen species, inflammation and protease activation, and strategies for prevention of inflammation-induced matrix degradation—a review. Int J Cosmet Sci 27:17–34
Voegeli R, Rawlings AV, Doppler S, Heiland J, Schreier T (2007) Profiling of serine protease activities in human stratum corneum and detection of a stratum corneum tryptase-like enzyme. Int J Cosmet Sci 29:191–200
Heinrikson RL, Kezdy FJ (1976) Acidic cysteine protease inhibitors from pineapple stem. Methods Enzymol 45:740–751
Rawlings ND, Barrett AJ (1994) Families of cysteine peptidases. Methods Enzymol 244:461–486
Walsh G, Headon DR (1994) Protein biotechnology. Wiley, New York
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Amid, A., Ismail, N., Jamaluddin, M. (2015). Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes. In: Amid, A. (eds) Recombinant Enzymes - From Basic Science to Commercialization. Springer, Cham. https://doi.org/10.1007/978-3-319-12397-4_11
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DOI: https://doi.org/10.1007/978-3-319-12397-4_11
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