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Recombinant Enzymes - From Basic Science to Commercialization pp 143–157Cite as

Case Study: Recombinant Bromelain Selection

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Abstract

This chapter presents an investigation that we performed prior to the decision to proceed cloning and producing recombinant bromelain. The criteria that we examined were the following: (1) easy access to a DNA source; (2) broad application; (3) an enzyme size amenable to the cloning strategy; (4) available data in free online databases; (5) broad industrial application.

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Authors and Affiliations

  1. Biomolecular and Bioprocess Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Azura Amid

  2. University Technology Mara, Campus Puncak Alam, Shah Alam, Selangor, Malaysia

    Nurul Azira Ismail

  3. Faculty of Chemical and Natural Resources Engineering, University Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia

    Zatul Iffah Mohd Arshad

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  1. Azura Amid
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  2. Nurul Azira Ismail
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  3. Zatul Iffah Mohd Arshad
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Correspondence to Azura Amid .

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Editors and Affiliations

  1. Department of Biotechnology Engineering Kulliyyah of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Azura Amid

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Amid, A., Ismail, N., Arshad, Z. (2015). Case Study: Recombinant Bromelain Selection. In: Amid, A. (eds) Recombinant Enzymes - From Basic Science to Commercialization. Springer, Cham. https://doi.org/10.1007/978-3-319-12397-4_10

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