Role of Src Family Kinases in Prolactin Signaling

  • Jorge Martín-PérezEmail author
  • José Manuel García-Martínez
  • María Pilar Sánchez-Bailón
  • Víctor Mayoral-Varo
  • Annarica Calcabrini
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 846)


Prolactin (PRL) is a polypeptide hormone/cytokine mainly synthesized by the lactotrophic cells of the adenohypophysis. In addition to the best-known role in mammary gland development and the functional differentiation of its epithelium, PRL is involved in regulation of multiple physiological processes in higher organisms contributing to their homeostasis. PRL has been also associated with pathology, including breast cancer. Therefore, it is relevant to determine the molecular mechanisms by which PRL controls cellular functions. Here, we analyze the role of Src family kinases (SFKs) in the intracellular signaling pathways controlled by PRL in several model systems. The data show that SFKs are essential components in transmitting signals upon PRL receptor stimulation, as they control activation of Jak2/Stat5 and other routes that regulate PRL cellular responses.


Conditional Expression Chick Embryo Fibroblast Ferm Domain Jak2 Activation PRLR Isoforms 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


BaF-3 cells

Mouse pro-B lymphocytes




Chick embryo fibroblasts


Carboxyl-terminus Src kinase


Epidermal growth factor


Epidermal growth factor receptor


Extracellular signal-regulated kinase 1/2


Focal adhesion kinase

FERM domain

F for 4.1 protein, E for erzin, R for radixin, and M for moesin


Fibroblast growth factor


Forkhead box O3 transcription factor


Growth hormone


Grb2-associated-binding protein 2


Growth factor receptor-bound protein 2


Glycogen synthase kinase 3beta


Immediate early genes


Interleukin 3


Insulin-like growth factor I receptor




Janus family kinases


Jak homology domain


Mitogen-activated protein kinase 1/2


Neuregulin differentiation factor


Platelet-derived growth factor




Prolactin receptor


Phosphoinositide 3-kinase


Replication-competent avian retroviral vectors derived from RSV


Rous Sarcoma Virus


Src family kinases


Src homology 3


Src homology 2


Tyrosine phosphatase containing two SH2 domains


Signal transducers and activators of transcription


Western blot

W53 cells

BaF-3 cells expressing the long form of the PRLR from rat ovary



We would like to thank former members of the group and collaborators for their experimental contributions, P.A. Kelly for his generous gift of PRLR antibody and PRLR cDNA, J.N. Ihle for the Jak2 cDNA, S. Roche and K. Ballmer for c-Src constructs, B.G. Neel and H. Gu for providing BaF-3-rt-Tet cells and Shp2 cDNA constructs, A.F. Parlow (NIDDK) for the supply of oPRL-20, and J Pérez for artwork. The authors acknowledge the support of the SAF2009-09254/SAF2012-38048 grant. We apologize to those colleagues whose work was not recognized.


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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Jorge Martín-Pérez
    • 1
    Email author
  • José Manuel García-Martínez
    • 1
    • 2
  • María Pilar Sánchez-Bailón
    • 1
  • Víctor Mayoral-Varo
    • 1
  • Annarica Calcabrini
    • 1
    • 3
  1. 1.Department of Cancer BiologyInstituto de Investigaciones Biomédicas A. Sols (CSIC/UAM)MadridSpain
  2. 2.Faculty of Health SciencesUniversidad Rey Juan Carlos IMadridSpain
  3. 3.Department of Technologies and HealthIstituto Superiore di SanitàRomeItaly

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