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Nanoparticles' Promises and Risks pp 303–333Cite as

Multi-dimensional Electrophoresis: The March in Pharma Applications

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Abstract

Electrophoresis is widely used in pharmaceutical field for multiple applications. It is mainly employed for protein separation and identification. The information obtained on protein content and composition from multiple gel electrophoresis provides basic knowledge required for drug design, biomarker, vaccine, and antibiotic research. Furthermore, combination with advance analytical techniques like MALDI-TOF, mass spectroscopy provides added advantages in analysing protien samples. Electrophoresis by single and multiple dimensional methods is becoming very popular in nanoparticle research to identify protein corona around particle which can predict organ targeting when used in vivo. This technique can also be used to detect toxicity potential of nanoparticles and generated oxidative stress. Multi-dimensional electrophoresis has already been used in conventional pharmaceutical application and now is marching to nanoparticle and/drug delivery research area.

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Abbreviations

1-DE:

1-Dimensional electrophoresis

2-DE:

2-Dimensional electrophoresis

2D-DIGE:

2-Dimensional differential in-gel electrophoresis

CE:

Capillary electrophoresis

CNTs:

Carbon Nano Tubes

CB:

Carbon black

CSF:

Cerebrospinal Fluid

IEF:

Immunofixation

IEF:

Isoelectric focussing

NEPHGE:

Nonequilibrium pH gel electrophoresis

PAGE:

Polyacrylamide gel electrophoresis

pI:

Isoelectric point

BAM:

N-tert-butylacrylamide

PIC:

Protease inhibitor cocktail

PMSF:

Phenyl methyl sulfonyl fluoride

PTMs:

Posttranslational modifications

SPE:

Serum protein electrophoresis

SDS:

Sodium dodecyl sulphate

TCA:

Trichloroacetic acid

KOH:

Potassium hydroxide

MPS:

Mononuclear phagocytic system

MS:

Mass spectroscopy

MWCNTs:

Multi-walled carbon nanotubes

NIPAM:

N-isopropylacrylamide

SILAC:

Stable Isotope labelling by amino acids in cell culture

ALP:

Alkaline phosphatase

LDH:

Lactate dehydrogenase

CK:

Creatine kinase

B. garinii :

Borrelia garinii

C. trachomatis :

Chlamydia trachomatis

E. coli :

Escherichia coli

H. pylori :

Helicobacter pylori

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Authors and Affiliations

  1. Department of Pharmaceutics, Biopharmaceutics and NutriCosmetics, Free University of Berlin, Kelchstr. 31, 12169, Berlin, Germany

    Ranjita Shegokar

  2. C.U. Shah College of Pharmacy, SNDT University, SantaCruz (W), 400049, Mumbai, India

    Sampada Sawant

Authors
  1. Ranjita Shegokar
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  2. Sampada Sawant
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Correspondence to Ranjita Shegokar .

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Editors and Affiliations

  1. Faculty of Physics, West University of Timisoara, Timisoara, Romania

    Mihai Lungu

  2. Faculty of Physics, West University of Timisoara, Timisoara, Romania

    Adrian Neculae

  3. Faculty of Physics, West University of Timisoara, Timisoara, Romania

    Madalin Bunoiu

  4. High Performance Computing Centre, West University of Timisoara, Timisoara, Romania

    Claudiu Biris

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Shegokar, R., Sawant, S. (2015). Multi-dimensional Electrophoresis: The March in Pharma Applications. In: Lungu, M., Neculae, A., Bunoiu, M., Biris, C. (eds) Nanoparticles' Promises and Risks. Springer, Cham. https://doi.org/10.1007/978-3-319-11728-7_15

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